Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NYS59_RS24395 Genome accession   NZ_CP103789
Coordinates   5235601..5236065 (-) Length   154 a.a.
NCBI ID   WP_058156624.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2718     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5230601..5241065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYS59_RS24375 nadC 5233102..5233950 (+) 849 WP_023435051.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NYS59_RS24385 - 5234131..5235060 (-) 930 WP_240462250.1 O-antigen ligase family protein -
  NYS59_RS24390 - 5235106..5235513 (-) 408 WP_316927926.1 hypothetical protein -
  NYS59_RS24395 pilA/pilAI 5235601..5236065 (-) 465 WP_058156624.1 pilin Machinery gene
  NYS59_RS24400 pilB 5236296..5237996 (+) 1701 WP_033956582.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NYS59_RS24405 pilC 5238000..5239217 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  NYS59_RS24410 pilD 5239218..5240090 (+) 873 WP_128652216.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  NYS59_RS24415 coaE 5240087..5240698 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  NYS59_RS24420 yacG 5240695..5240895 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16079.36 Da        Isoelectric Point: 8.4551

>NTDB_id=625455 NYS59_RS24395 WP_058156624.1 5235601..5236065(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2718]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGSGKDQIKIDKADTATPEISGTLGGSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=625455 NYS59_RS24395 WP_058156624.1 5235601..5236065(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2718]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTAGCGGTAAGGATCAGATCAAAATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCGGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.161

100

0.455
  pilA Acinetobacter baumannii strain A118

43.709

98.052

0.429
  pilA Pseudomonas aeruginosa PAK

41.772

100

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.411

98.052

0.377
  pilA Vibrio cholerae strain A1552

38.411

98.052

0.377
  pilA Vibrio cholerae C6706

38.411

98.052

0.377
  pilA2 Legionella pneumophila strain ERS1305867

38.514

96.104

0.37
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364