Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KFS85_RS05065 Genome accession   NZ_CP086332
Coordinates   1195212..1196471 (-) Length   419 a.a.
NCBI ID   WP_237653168.1    Uniprot ID   -
Organism   Xanthomonas graminis pv. phlei strain LMG 843     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1190212..1201471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFS85_RS05040 (KFS85_05050) - 1191288..1191482 (+) 195 WP_237653163.1 hypothetical protein -
  KFS85_RS05045 (KFS85_05055) - 1191581..1192258 (+) 678 WP_003466405.1 response regulator transcription factor -
  KFS85_RS05050 (KFS85_05060) - 1192251..1193585 (+) 1335 WP_053838010.1 sensor histidine kinase -
  KFS85_RS05055 (KFS85_05065) coaE 1193708..1194328 (-) 621 WP_237653164.1 dephospho-CoA kinase -
  KFS85_RS05060 (KFS85_05070) - 1194342..1195205 (-) 864 WP_237653166.1 prepilin peptidase -
  KFS85_RS05065 (KFS85_05075) pilC 1195212..1196471 (-) 1260 WP_237653168.1 type II secretion system F family protein Machinery gene
  KFS85_RS05070 (KFS85_05080) - 1196860..1197297 (+) 438 WP_237653169.1 pilin -
  KFS85_RS05075 (KFS85_05085) - 1197443..1198138 (-) 696 WP_237653171.1 transposase -
  KFS85_RS05080 (KFS85_05090) - 1198274..1198645 (-) 372 WP_053838005.1 DUF86 domain-containing protein -
  KFS85_RS05085 (KFS85_05095) - 1198642..1198932 (-) 291 WP_009580418.1 nucleotidyltransferase family protein -
  KFS85_RS05090 (KFS85_05100) pilB 1199139..1200875 (+) 1737 WP_237653172.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45562.75 Da        Isoelectric Point: 10.1825

>NTDB_id=624337 KFS85_RS05065 WP_237653168.1 1195212..1196471(-) (pilC) [Xanthomonas graminis pv. phlei strain LMG 843]
MSTTRSAVSKEPVARNISQQVPFIWEGTDKRGIKMKGEQTAKNANLLRAELRRQGITPSVVKPKPKPLFGAAGSKISAKD
IAFFSRQMATMMKSGVPIVGSLEIIGSGHKNPRMKKMVGQVRTDIEGGSSLYEAISKHPVQFDELYRNLVKAGEGAGVLE
TVLETVATYKENTEALKGKIKKALFYPAAVMAVALLVSSILLVWVVPQFEDVFKGFGAELPAFTQMIVAASRFMVAYWWL
LLIVLIGSIVGFVFAYKRSPSMQHGMDRLILKVPIIGQIMHNSSVARFSRTLGVTFRAGVPLVEALDIVAGATGNSVYEK
AVLRMRDDVSVGYPVNVAMKQANLFPHMVIQMTAIGEEAGALDAMLFKVAEYFEQEVNNAVDALSSLLEPLIMVFIGTIV
GGMVVGMYLPIFKLASVVG

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=624337 KFS85_RS05065 WP_237653168.1 1195212..1196471(-) (pilC) [Xanthomonas graminis pv. phlei strain LMG 843]
ATGTCCACAACTCGTAGCGCAGTATCCAAAGAACCCGTCGCACGCAATATCAGCCAGCAGGTGCCGTTCATCTGGGAAGG
GACGGACAAGCGCGGCATCAAGATGAAGGGCGAGCAGACGGCCAAGAACGCCAACCTGCTGCGTGCGGAATTGCGCCGCC
AGGGCATCACGCCGTCCGTGGTCAAGCCGAAACCCAAACCCCTGTTCGGCGCCGCCGGCAGCAAGATCAGTGCGAAGGAC
ATTGCCTTCTTCAGCCGCCAGATGGCGACCATGATGAAGTCGGGTGTCCCCATCGTGGGATCGCTGGAGATCATCGGCAG
CGGGCACAAGAACCCGCGCATGAAGAAGATGGTGGGACAGGTCCGCACGGACATCGAAGGCGGCTCGTCGCTCTATGAAG
CGATCAGCAAGCATCCGGTCCAGTTCGATGAGCTCTACCGCAACCTGGTCAAGGCCGGCGAAGGCGCCGGCGTACTCGAA
ACCGTGCTGGAGACGGTAGCCACCTACAAGGAAAACACCGAGGCGCTGAAGGGCAAGATCAAGAAAGCCTTGTTCTATCC
GGCCGCGGTCATGGCCGTGGCGCTCCTGGTCAGCTCGATCTTGCTGGTGTGGGTGGTCCCGCAATTCGAGGATGTCTTCA
AAGGGTTTGGCGCGGAGTTACCCGCATTCACTCAGATGATCGTCGCCGCTTCACGCTTTATGGTGGCTTATTGGTGGCTC
CTGCTGATCGTGCTGATTGGCAGCATCGTCGGATTCGTCTTCGCCTACAAGCGCTCGCCGTCGATGCAGCACGGCATGGA
CCGGCTGATCCTGAAGGTGCCGATCATCGGCCAGATCATGCACAACAGCTCGGTGGCACGCTTCTCGCGGACACTGGGCG
TCACCTTCCGCGCCGGCGTCCCGTTGGTGGAAGCGCTGGACATCGTCGCCGGCGCCACCGGCAACAGCGTCTACGAGAAG
GCCGTACTGCGCATGCGTGACGATGTCTCGGTGGGCTACCCGGTCAACGTGGCGATGAAACAGGCCAACCTGTTTCCGCA
CATGGTCATCCAGATGACCGCGATCGGCGAAGAAGCCGGTGCGCTGGATGCGATGCTGTTCAAGGTGGCCGAGTACTTCG
AGCAGGAGGTGAACAATGCGGTCGATGCGTTGAGTAGCCTGCTGGAACCGCTGATCATGGTGTTCATCGGCACCATCGTC
GGCGGCATGGTCGTCGGCATGTACCTGCCCATCTTCAAGCTGGCCTCCGTCGTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.283

94.511

0.504
  pilC Legionella pneumophila strain ERS1305867

51.733

96.42

0.499
  pilC Acinetobacter baylyi ADP1

51.117

96.181

0.492
  pilC Acinetobacter baumannii D1279779

50.62

96.181

0.487
  pilG Neisseria gonorrhoeae MS11

43.216

94.988

0.411
  pilC Vibrio cholerae strain A1552

42.222

96.659

0.408
  pilG Neisseria meningitidis 44/76-A

42.714

94.988

0.406
  pilC Vibrio campbellii strain DS40M4

39.798

94.749

0.377
  pilC Thermus thermophilus HB27

38.5

95.465

0.368