Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KFS85_RS05090 Genome accession   NZ_CP086332
Coordinates   1199139..1200875 (+) Length   578 a.a.
NCBI ID   WP_237653172.1    Uniprot ID   -
Organism   Xanthomonas graminis pv. phlei strain LMG 843     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1194139..1205875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFS85_RS05060 (KFS85_05070) - 1194342..1195205 (-) 864 WP_237653166.1 prepilin peptidase -
  KFS85_RS05065 (KFS85_05075) pilC 1195212..1196471 (-) 1260 WP_237653168.1 type II secretion system F family protein Machinery gene
  KFS85_RS05070 (KFS85_05080) - 1196860..1197297 (+) 438 WP_237653169.1 pilin -
  KFS85_RS05075 (KFS85_05085) - 1197443..1198138 (-) 696 WP_237653171.1 transposase -
  KFS85_RS05080 (KFS85_05090) - 1198274..1198645 (-) 372 WP_053838005.1 DUF86 domain-containing protein -
  KFS85_RS05085 (KFS85_05095) - 1198642..1198932 (-) 291 WP_009580418.1 nucleotidyltransferase family protein -
  KFS85_RS05090 (KFS85_05100) pilB 1199139..1200875 (+) 1737 WP_237653172.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFS85_RS05095 (KFS85_05105) - 1200885..1201502 (-) 618 WP_237653174.1 Fic/DOC family protein -
  KFS85_RS05100 (KFS85_05110) - 1201499..1201681 (-) 183 WP_003477093.1 YhfG family protein -
  KFS85_RS05105 (KFS85_05115) pilR 1201869..1203257 (-) 1389 WP_237653175.1 sigma-54-dependent transcriptional regulator Regulator
  KFS85_RS05110 (KFS85_05120) - 1203332..1204945 (-) 1614 WP_003477089.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62855.12 Da        Isoelectric Point: 6.3627

>NTDB_id=624338 KFS85_RS05090 WP_237653172.1 1199139..1200875(+) (pilB) [Xanthomonas graminis pv. phlei strain LMG 843]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELVHKHHVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGKLEVGAGDEDMGSGGDTGVDAKGDDTPVVKFVNKILVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=624338 KFS85_RS05090 WP_237653172.1 1199139..1200875(+) (pilB) [Xanthomonas graminis pv. phlei strain LMG 843]
ATGAACGCTGTGACATCCGCCAATCTCGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAATGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGGTGCACAAGCACCATGTGCTGCCGCTGTTCAAGCGCGGCAACCGTCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCTATGGGCAAGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATACTGGTCGATGCGATCCGCCGCGGCGCCTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGGCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATCGCGGCGCGGTTGAAGGTAATGTCGCAGTTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACGCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACGGCCGAAGACCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACAGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAATTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.106

98.27

0.542
  pilB Acinetobacter baumannii D1279779

54.276

99.135

0.538
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilB Vibrio cholerae strain A1552

48.873

99.827

0.488
  pilF Neisseria gonorrhoeae MS11

48.944

98.27

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

47.173

97.924

0.462
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.421

90.311

0.365
  pilF Thermus thermophilus HB27

40.192

89.965

0.362