Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LA387_RS07360 Genome accession   NZ_CP084377
Coordinates   1435618..1436562 (-) Length   314 a.a.
NCBI ID   WP_003133715.1    Uniprot ID   -
Organism   Lactococcus garvieae strain Lg-Granada     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1430618..1441562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA387_RS07325 - 1431226..1432095 (+) 870 WP_003133725.1 RluA family pseudouridine synthase -
  LA387_RS07330 - 1432181..1433026 (-) 846 WP_003133722.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LA387_RS07335 comGF 1433259..1433699 (-) 441 WP_003133720.1 competence type IV pilus minor pilin ComGF -
  LA387_RS07340 comGE 1433680..1433913 (-) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  LA387_RS07345 comGD 1433945..1434361 (-) 417 WP_003133718.1 competence type IV pilus minor pilin ComGD Machinery gene
  LA387_RS07350 comYC 1434336..1434641 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  LA387_RS07355 comGB 1434660..1435679 (-) 1020 WP_263867708.1 competence type IV pilus assembly protein ComGB Machinery gene
  LA387_RS07360 comGA 1435618..1436562 (-) 945 WP_003133715.1 competence type IV pilus ATPase ComGA Machinery gene
  LA387_RS07365 rpsU 1436750..1436926 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  LA387_RS07370 guaB 1437049..1438530 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  LA387_RS07375 - 1438699..1440300 (+) 1602 WP_225666504.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35836.07 Da        Isoelectric Point: 6.6641

>NTDB_id=610865 LA387_RS07360 WP_003133715.1 1435618..1436562(-) (comGA) [Lactococcus garvieae strain Lg-Granada]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=610865 LA387_RS07360 WP_003133715.1 1435618..1436562(-) (comGA) [Lactococcus garvieae strain Lg-Granada]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGGGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTCATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACAGTATTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTGCACGG
CTAAAGGAACTTGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGATTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.554

100

0.596
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

51.603

99.363

0.513
  comYA Streptococcus mutans UA140

51.603

99.363

0.513