Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEO78_RS17840 Genome accession   NZ_CP084353
Coordinates   3949278..3950387 (+) Length   369 a.a.
NCBI ID   WP_016351972.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 71317     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3944278..3955387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO78_RS17805 (LEO78_17805) - 3944894..3945205 (+) 312 WP_226032369.1 hypothetical protein -
  LEO78_RS17810 (LEO78_17810) - 3945262..3945681 (-) 420 WP_202004565.1 DUF4426 domain-containing protein -
  LEO78_RS17815 (LEO78_17815) yggU 3945700..3945999 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  LEO78_RS17820 (LEO78_17820) - 3945999..3946550 (-) 552 WP_016351969.1 YggT family protein -
  LEO78_RS17825 (LEO78_17825) proC 3946591..3947415 (-) 825 WP_226032370.1 pyrroline-5-carboxylate reductase -
  LEO78_RS17830 (LEO78_17830) - 3947474..3948175 (-) 702 WP_226032371.1 YggS family pyridoxal phosphate-dependent enzyme -
  LEO78_RS17835 (LEO78_17835) pilT 3948215..3949249 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  LEO78_RS17840 (LEO78_17840) pilU 3949278..3950387 (+) 1110 WP_016351972.1 type IVa pilus ATPase TapU Machinery gene
  LEO78_RS17845 (LEO78_17845) yaaA 3950419..3951192 (+) 774 WP_101615569.1 peroxide stress protein YaaA -
  LEO78_RS17850 (LEO78_17850) srmB 3951369..3952592 (-) 1224 WP_226032372.1 ATP-dependent RNA helicase SrmB -
  LEO78_RS17855 (LEO78_17855) - 3952721..3953431 (+) 711 WP_226032373.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  LEO78_RS17860 (LEO78_17860) brnQ 3953623..3954930 (+) 1308 WP_043146375.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41054.21 Da        Isoelectric Point: 6.5657

>NTDB_id=610813 LEO78_RS17840 WP_016351972.1 3949278..3950387(+) (pilU) [Aeromonas hydrophila strain 71317]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=610813 LEO78_RS17840 WP_016351972.1 3949278..3950387(+) (pilU) [Aeromonas hydrophila strain 71317]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGACCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTACAGGAGATTGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTAGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCGTTGCGCCAGGCCCCTGACGTGATACTGATCGGCGAAATCCGCAGCCAGGAAACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAATCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGCCGCTGTGCAGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCTGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36