Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   LEO81_RS02330 Genome accession   NZ_CP084351
Coordinates   503499..503912 (-) Length   137 a.a.
NCBI ID   WP_226014238.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain 71431     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498499..508912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO81_RS02310 (LEO81_02310) coaE 498897..499511 (-) 615 WP_017766418.1 dephospho-CoA kinase -
  LEO81_RS02315 (LEO81_02315) pilD 499531..500403 (-) 873 WP_226014235.1 A24 family peptidase Machinery gene
  LEO81_RS02320 (LEO81_02320) pilC 500426..501667 (-) 1242 WP_226014236.1 type II secretion system F family protein Machinery gene
  LEO81_RS02325 (LEO81_02325) pilB 501783..503489 (-) 1707 WP_226014237.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LEO81_RS02330 (LEO81_02330) pilA2 503499..503912 (-) 414 WP_226014238.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LEO81_RS02335 (LEO81_02335) nadC 504233..505090 (-) 858 WP_226014239.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LEO81_RS02340 (LEO81_02340) - 505095..505565 (-) 471 WP_010635963.1 TIGR02281 family clan AA aspartic protease -
  LEO81_RS02345 (LEO81_02345) ampD 505689..506261 (+) 573 WP_226014240.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LEO81_RS02350 (LEO81_02350) pdhR 506537..507301 (+) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 13604.44 Da        Isoelectric Point: 7.5902

>NTDB_id=610747 LEO81_RS02330 WP_226014238.1 503499..503912(-) (pilA2) [Aeromonas dhakensis strain 71431]
MKKQSGFTLIELMIVVAIVAILAAIALPAYQDYTRRARATEVVVATGGYKTGVEVCAQSTQTLTGCDAGTNGIPAAISGA
SGTSKVATMSVTNGVITGTGTADTGTGGTGCSISLTPTIDNGRVLWGPTSGATNNCS

Nucleotide


Download         Length: 414 bp        

>NTDB_id=610747 LEO81_RS02330 WP_226014238.1 503499..503912(-) (pilA2) [Aeromonas dhakensis strain 71431]
ATGAAGAAACAATCAGGCTTTACCCTTATCGAATTGATGATCGTGGTCGCGATCGTCGCCATCCTGGCCGCCATTGCGCT
GCCGGCGTATCAGGACTACACACGCAGAGCTCGGGCAACCGAAGTTGTCGTTGCTACTGGGGGATATAAAACTGGTGTAG
AAGTTTGCGCACAATCCACTCAAACCCTCACAGGATGCGATGCAGGTACAAACGGAATACCGGCAGCAATCTCAGGAGCG
AGTGGAACCAGTAAAGTAGCAACTATGAGCGTCACAAACGGTGTTATTACTGGCACAGGAACTGCGGATACAGGAACAGG
TGGAACAGGTTGCTCTATTAGCTTAACACCAACTATTGATAATGGCCGTGTATTATGGGGACCCACCTCTGGGGCCACAA
ATAACTGCTCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-130)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

50

96.35

0.482
  pilA2 Legionella pneumophila strain ERS1305867

49.242

96.35

0.474
  comP Acinetobacter baylyi ADP1

41.176

99.27

0.409
  pilA Pseudomonas aeruginosa PAK

38.462

100

0.401
  pilE Neisseria gonorrhoeae MS11

41.732

92.701

0.387
  pilA Haemophilus influenzae 86-028NP

52.577

70.803

0.372