Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LEO81_RS02325 Genome accession   NZ_CP084351
Coordinates   501783..503489 (-) Length   568 a.a.
NCBI ID   WP_226014237.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain 71431     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496783..508489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO81_RS02295 (LEO81_02295) mutT 497458..497871 (+) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -
  LEO81_RS02300 (LEO81_02300) yacG 497933..498127 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  LEO81_RS02305 (LEO81_02305) zapD 498137..498859 (-) 723 WP_025202564.1 cell division protein ZapD -
  LEO81_RS02310 (LEO81_02310) coaE 498897..499511 (-) 615 WP_017766418.1 dephospho-CoA kinase -
  LEO81_RS02315 (LEO81_02315) pilD 499531..500403 (-) 873 WP_226014235.1 A24 family peptidase Machinery gene
  LEO81_RS02320 (LEO81_02320) pilC 500426..501667 (-) 1242 WP_226014236.1 type II secretion system F family protein Machinery gene
  LEO81_RS02325 (LEO81_02325) pilB 501783..503489 (-) 1707 WP_226014237.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LEO81_RS02330 (LEO81_02330) pilA2 503499..503912 (-) 414 WP_226014238.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LEO81_RS02335 (LEO81_02335) nadC 504233..505090 (-) 858 WP_226014239.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LEO81_RS02340 (LEO81_02340) - 505095..505565 (-) 471 WP_010635963.1 TIGR02281 family clan AA aspartic protease -
  LEO81_RS02345 (LEO81_02345) ampD 505689..506261 (+) 573 WP_226014240.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LEO81_RS02350 (LEO81_02350) pdhR 506537..507301 (+) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62540.90 Da        Isoelectric Point: 5.4321

>NTDB_id=610746 LEO81_RS02325 WP_226014237.1 501783..503489(-) (pilB) [Aeromonas dhakensis strain 71431]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLTEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=610746 LEO81_RS02325 WP_226014237.1 501783..503489(-) (pilB) [Aeromonas dhakensis strain 71431]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAAAACGACATCCTCGACAGCAAGGCGCTGG
CCGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGACCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGACACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAAGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAACGATCAGGACGCCCTCGGCATGGAGGACATCGACGAGTCCGAAATT
TCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTTGAGCCCTATGAGACCAAGTACC
GCATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAGCTGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGCCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGGCAGAAAGCGCAATATCTGCGCGCGCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCAACCCCAAGG
CCGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTAGTGATGGTCGGTGAAATACGGGAT
CTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAACTCGGCGGC
CGAGACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCATGGCTCAGC
GACTGGCACGCAAGCTGTGCGACCACTGCAAGGCCCCCGAAGTGGTGCCGGAAGCTGAACTGCTGGAGTTGGGGTTTACC
CAACAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAGCCGGTTGGTTGCAAGGAGTGTTCGGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAGATCCTGTTGATGTCCGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGCAGATTGCGG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.009

100

0.551
  pilB Acinetobacter baylyi ADP1

54.912

100

0.551
  pilB Legionella pneumophila strain ERS1305867

54.417

99.648

0.542
  pilB Vibrio cholerae strain A1552

53.805

99.472

0.535
  pilB Vibrio campbellii strain DS40M4

51.59

99.648

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.944

0.504
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilF Thermus thermophilus HB27

40.53

92.958

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

38.517

97.359

0.375