Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LDZ27_RS02450 Genome accession   NZ_CP084265
Coordinates   516654..517862 (-) Length   402 a.a.
NCBI ID   WP_244815162.1    Uniprot ID   -
Organism   Caballeronia sp. Lep1P3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511654..522862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDZ27_RS02420 mutT 512233..512673 (+) 441 WP_244815157.1 8-oxo-dGTP diphosphatase MutT -
  LDZ27_RS02425 yacG 512687..512878 (-) 192 WP_244815158.1 DNA gyrase inhibitor YacG -
  LDZ27_RS02430 zapD 512959..513714 (-) 756 WP_244816014.1 cell division protein ZapD -
  LDZ27_RS02435 coaE 513885..514490 (-) 606 WP_244815159.1 dephospho-CoA kinase -
  LDZ27_RS02440 - 514504..515415 (-) 912 WP_244815160.1 A24 family peptidase -
  LDZ27_RS02445 - 515446..516654 (-) 1209 WP_244815161.1 type II secretion system F family protein -
  LDZ27_RS02450 pilB 516654..517862 (-) 1209 WP_244815162.1 GspE/PulE family protein Machinery gene
  LDZ27_RS02455 - 517937..519223 (-) 1287 WP_244815163.1 HlyC/CorC family transporter -
  LDZ27_RS02465 - 519475..520467 (-) 993 WP_244815164.1 polyprenyl synthetase family protein -
  LDZ27_RS02470 rplU 520714..521025 (+) 312 WP_244815165.1 50S ribosomal protein L21 -
  LDZ27_RS02475 rpmA 521069..521329 (+) 261 WP_244815166.1 50S ribosomal protein L27 -
  LDZ27_RS02480 cgtA 521519..522622 (+) 1104 WP_244815167.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43103.41 Da        Isoelectric Point: 7.4671

>NTDB_id=610370 LDZ27_RS02450 WP_244815162.1 516654..517862(-) (pilB) [Caballeronia sp. Lep1P3]
MTSASRDAFRPPARAPEHAFDAASEQDAPAVSLLADTLREAAERGASDIHVEPSEHEWRIRLRVDGALHVLRRPPPHLRD
AFITRIKVLARMDIAERRVPQDGRLRLALPRGETGDYRVNSLPTLFGEKLVLRRLDSLPPDLSLGALGLDAKQSATVDSA
IRAPHGLILVTGPTGSGKTLSLYCFLQMLNAEARNVCSVEDPVEIQLAGINQVSVREKAGLTFAVALRAFMRQDPDVIMV
GEIRDAETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVAPYNLASALRLVTAQRLVRKLCEACRAPSALALADGF
CPFEPRGCPACHGIGYRGRVGVHQLMPVSDSLRELIVSRSATHAIARAAQSEGMPALRDAASARVREGVTSVAEAAEATE
VA

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=610370 LDZ27_RS02450 WP_244815162.1 516654..517862(-) (pilB) [Caballeronia sp. Lep1P3]
ATGACTTCCGCCTCCCGCGACGCTTTTCGCCCGCCCGCTCGCGCTCCCGAGCATGCTTTCGATGCCGCGTCCGAACAGGA
CGCGCCCGCCGTGTCGCTCCTCGCCGATACGTTGCGCGAAGCCGCCGAGCGCGGCGCATCCGATATCCACGTCGAGCCTT
CGGAGCACGAATGGCGCATCCGGCTGCGCGTCGATGGCGCGCTGCACGTCTTGCGCCGTCCGCCGCCGCATTTGCGCGAC
GCGTTCATCACGCGCATCAAGGTGCTGGCGCGCATGGACATCGCCGAGCGGCGCGTGCCGCAGGACGGTCGTTTGCGGCT
CGCTTTGCCGCGCGGCGAGACCGGCGACTATCGCGTGAATTCGCTGCCGACGCTCTTCGGCGAGAAGCTCGTCCTGCGAC
GGCTGGATTCGTTGCCGCCCGACTTGTCGCTCGGCGCGCTCGGCCTCGACGCCAAGCAAAGCGCCACCGTCGATTCGGCG
ATTCGCGCGCCGCACGGCCTCATTCTGGTGACGGGACCGACGGGCAGCGGCAAGACCTTGTCGCTCTACTGCTTTCTTCA
GATGCTCAACGCGGAGGCGCGCAACGTCTGTTCGGTCGAAGATCCGGTCGAAATTCAACTGGCCGGCATCAATCAGGTGA
GCGTGCGCGAGAAAGCCGGCCTGACTTTCGCGGTGGCGCTGCGCGCGTTCATGCGACAGGACCCGGACGTCATCATGGTC
GGGGAAATTCGCGACGCCGAAACCGCCGATGTCGCCGTGAAAGCCGCGCAAACCGGCCATCTCGTGCTCTCGACGCTGCA
CACGAACGACGCGCCCGCCGCCATCGCGCGACTCATCGATATCGGCGTCGCGCCGTACAACCTCGCGTCCGCGCTGCGGC
TCGTGACCGCGCAGCGCCTGGTGCGCAAGCTGTGCGAGGCGTGCCGCGCGCCGTCCGCGCTCGCGCTCGCGGACGGCTTT
TGTCCGTTCGAGCCGCGCGGGTGTCCGGCGTGCCACGGCATCGGGTATCGCGGGCGTGTCGGCGTGCATCAGCTGATGCC
GGTCTCCGACAGCCTGCGCGAGCTAATCGTCTCGCGCTCGGCGACGCACGCCATCGCGCGCGCCGCGCAATCCGAAGGCA
TGCCCGCCTTGCGCGATGCCGCGTCCGCGCGCGTGCGCGAAGGCGTGACGAGCGTGGCCGAAGCCGCTGAAGCAACGGAA
GTCGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

50.386

96.766

0.488
  pilF Neisseria gonorrhoeae MS11

48.232

98.507

0.475
  pilB Acinetobacter baumannii D1279779

47.619

99.254

0.473
  pilB Acinetobacter baylyi ADP1

48.446

96.02

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

47.632

94.527

0.45
  pilB Vibrio campbellii strain DS40M4

47.632

94.527

0.45
  pilB Legionella pneumophila strain ERS1305867

46.891

96.02

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

43.796

100

0.448
  pilB Glaesserella parasuis strain SC1401

41.795

97.015

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.967

97.761

0.4
  pilF Thermus thermophilus HB27

41.579

94.527

0.393
  pilB Haemophilus influenzae Rd KW20

42.133

93.284

0.393
  pilB Haemophilus influenzae 86-028NP

41.6

93.284

0.388
  ctsE Campylobacter jejuni subsp. jejuni 81-176

38.978

92.537

0.361