Detailed information
Bioinformatically predicted
Overview
| Name | pilA/pilAI | Type | Machinery gene |
| Locus tag | NLS69_RS24520 | Genome accession | NZ_CP100653 |
| Coordinates | 5235879..5236346 (-) | Length | 155 a.a. |
| NCBI ID | WP_003161762.1 | Uniprot ID | - |
| Organism | Pseudomonas aeruginosa strain F13 | ||
| Function | assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Genomic Context
Location: 5230879..5241346
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NLS69_RS24505 (NLS69_24505) | nadC | 5233379..5234227 (+) | 849 | WP_017148538.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
| NLS69_RS24515 (NLS69_24515) | - | 5234408..5235721 (-) | 1314 | WP_227352480.1 | O-antigen ligase | - |
| NLS69_RS24520 (NLS69_24520) | pilA/pilAI | 5235879..5236346 (-) | 468 | WP_003161762.1 | pilin | Machinery gene |
| NLS69_RS24525 (NLS69_24525) | pilB | 5236577..5238277 (+) | 1701 | WP_003107297.1 | type IV-A pilus assembly ATPase PilB | Machinery gene |
| NLS69_RS24530 (NLS69_24530) | pilC | 5238281..5239498 (+) | 1218 | WP_003107299.1 | type II secretion system F family protein | Machinery gene |
| NLS69_RS24535 (NLS69_24535) | pilD | 5239499..5240371 (+) | 873 | WP_003107301.1 | type IV prepilin peptidase/methyltransferase PilD | Machinery gene |
| NLS69_RS24540 (NLS69_24540) | coaE | 5240368..5240979 (+) | 612 | WP_003112838.1 | dephospho-CoA kinase | - |
| NLS69_RS24545 (NLS69_24545) | yacG | 5240976..5241176 (+) | 201 | WP_003094656.1 | DNA gyrase inhibitor YacG | - |
Sequence
Protein
Download Length: 155 a.a. Molecular weight: 15959.30 Da Isoelectric Point: 9.0145
>NTDB_id=605972 NLS69_RS24520 WP_003161762.1 5235879..5236346(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F13]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS
Nucleotide
Download Length: 468 bp
>NTDB_id=605972 NLS69_RS24520 WP_003161762.1 5235879..5236346(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F13]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA/pilAI | Pseudomonas stutzeri DSM 10701 |
45.679 |
100 |
0.477 |
| pilA | Pseudomonas aeruginosa PAK |
40.964 |
100 |
0.439 |
| pilA | Acinetobacter baumannii strain A118 |
43.312 |
100 |
0.439 |
| pilA | Ralstonia pseudosolanacearum GMI1000 |
37.427 |
100 |
0.413 |
| pilA | Vibrio cholerae O1 biovar El Tor strain E7946 |
43.262 |
90.968 |
0.394 |
| pilA | Vibrio cholerae strain A1552 |
43.262 |
90.968 |
0.394 |
| pilA | Vibrio cholerae C6706 |
43.262 |
90.968 |
0.394 |
| pilA/pilAII | Pseudomonas stutzeri DSM 10701 |
39.333 |
96.774 |
0.381 |
| pilA/pilA1 | Eikenella corrodens VA1 |
38.095 |
94.839 |
0.361 |