Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   K8O84_RS05670 Genome accession   NZ_CP082862
Coordinates   1177315..1178580 (+) Length   421 a.a.
NCBI ID   WP_061955312.1    Uniprot ID   -
Organism   Cupriavidus pauculus strain FDAARGOS_1472     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1172315..1183580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O84_RS05655 (K8O84_05625) ispB 1174084..1175013 (+) 930 WP_061955316.1 octaprenyl diphosphate synthase -
  K8O84_RS05665 (K8O84_05635) pilB 1175563..1177287 (+) 1725 WP_061955314.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K8O84_RS05670 (K8O84_05640) pilG 1177315..1178580 (+) 1266 WP_061955312.1 type II secretion system F family protein Machinery gene
  K8O84_RS05675 (K8O84_05645) - 1178599..1179492 (+) 894 WP_150979518.1 A24 family peptidase -
  K8O84_RS05680 (K8O84_05650) coaE 1179544..1180167 (+) 624 WP_150979517.1 dephospho-CoA kinase -
  K8O84_RS05685 (K8O84_05655) zapD 1180419..1181177 (+) 759 WP_061955303.1 cell division protein ZapD -
  K8O84_RS05690 (K8O84_05660) - 1181183..1181374 (+) 192 WP_061955301.1 DNA gyrase inhibitor YacG -
  K8O84_RS05695 (K8O84_05665) - 1181392..1183422 (-) 2031 WP_150979516.1 OPT family oligopeptide transporter -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46555.65 Da        Isoelectric Point: 10.2115

>NTDB_id=603223 K8O84_RS05670 WP_061955312.1 1177315..1178580(+) (pilG) [Cupriavidus pauculus strain FDAARGOS_1472]
MATRAPAASARTAAPSRAKSGQKAPTQYIFEWEAKDRKGKSFKGEQRAESQAEVTATLRKQGLTVVKMKKRKAARGKKIT
EKDIAYFTRQLSTMLKAGIPLLQSIDIIARGHANPNFTQLLNIIRFDIEAGSSMATAFRRHPRYFDTLYCNLIDAGEQGG
ILDALLERLSLYMEKSIALKSQIKSAMIYPIAVLTVAFAVTVVLMIFVIPAFKGVFSSFGAALPAPTQMVIGMSDFFVDH
WYLIIGVPIFSIIGYIHARRKSEKVQRWHDKALLRMPIFGSLFRKAVIARWTRTLATMFAAGTPLVESMESVAGAAGNWL
YYDATREIEQSVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVAAISSLIEPLIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=603223 K8O84_RS05670 WP_061955312.1 1177315..1178580(+) (pilG) [Cupriavidus pauculus strain FDAARGOS_1472]
ATGGCGACGCGCGCACCAGCGGCGAGCGCCCGGACGGCAGCGCCGTCGCGGGCCAAATCGGGGCAGAAGGCGCCCACGCA
ATACATCTTTGAATGGGAAGCCAAGGACCGCAAAGGCAAGTCCTTCAAGGGCGAGCAGCGGGCCGAGAGCCAGGCCGAAG
TCACGGCCACCCTGCGCAAGCAGGGGCTGACCGTCGTCAAGATGAAAAAGCGCAAGGCGGCGCGCGGCAAGAAGATCACC
GAAAAGGACATCGCGTACTTCACGCGCCAGCTGTCGACAATGCTCAAGGCCGGCATTCCGCTGCTGCAGTCCATCGACAT
CATCGCGCGCGGCCACGCCAACCCGAATTTCACGCAGCTGCTCAACATCATCCGCTTCGACATCGAAGCCGGTAGCAGCA
TGGCCACGGCGTTCCGCCGCCATCCGCGCTACTTCGACACGCTCTATTGCAATCTCATCGACGCCGGCGAACAGGGCGGT
ATTCTCGACGCCCTGCTGGAACGCCTTTCGCTCTACATGGAAAAGAGCATCGCGCTGAAGAGCCAGATCAAGTCCGCGAT
GATCTATCCGATCGCGGTGCTGACCGTGGCCTTTGCCGTGACCGTGGTGTTGATGATCTTCGTGATCCCGGCCTTCAAGG
GGGTGTTCTCAAGCTTCGGCGCCGCGCTGCCCGCGCCCACCCAGATGGTCATCGGCATGTCCGACTTCTTTGTCGACCAC
TGGTATCTGATCATTGGCGTGCCGATCTTCTCCATCATCGGCTACATCCACGCGCGCAGAAAATCGGAAAAGGTGCAGCG
CTGGCATGACAAGGCGCTGCTCAGGATGCCGATCTTTGGCAGCCTGTTCCGCAAGGCCGTGATTGCCCGCTGGACGCGCA
CGCTGGCCACGATGTTCGCCGCCGGCACGCCGCTGGTGGAATCGATGGAGTCCGTGGCGGGCGCCGCGGGCAACTGGCTG
TACTACGACGCCACGCGCGAGATCGAACAGTCGGTGCGCATCGGCACCAGCCTGACCAACGCGATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACACAAATTGGTGAGGAATCTGGCGCGCTCGACAACATGCTGCTCAAGGTGGCCG
AGTTTTACGAGCGCGAAGTCGACGACGCCGTGGCCGCCATTTCCAGCCTGATCGAGCCGCTGATCATCGTCATTCTTGGC
GTGCTGATCGGCGGCATGGTGGTGGCCATGTATCTGCCGATCTTCAAGCTGGGCCAGGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.122

95.249

0.487
  pilG Neisseria meningitidis 44/76-A

50.623

95.249

0.482
  pilC Pseudomonas stutzeri DSM 10701

49.38

95.724

0.473
  pilC Acinetobacter baumannii D1279779

48.039

96.912

0.466
  pilC Acinetobacter baylyi ADP1

47.677

97.15

0.463
  pilC Legionella pneumophila strain ERS1305867

47.525

95.962

0.456
  pilC Vibrio campbellii strain DS40M4

39.851

95.962

0.382
  pilC Vibrio cholerae strain A1552

38.329

96.675

0.371
  pilC Thermus thermophilus HB27

36.779

98.812

0.363