Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K8O84_RS05665 Genome accession   NZ_CP082862
Coordinates   1175563..1177287 (+) Length   574 a.a.
NCBI ID   WP_061955314.1    Uniprot ID   -
Organism   Cupriavidus pauculus strain FDAARGOS_1472     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1170563..1182287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O84_RS05650 (K8O84_05620) ltrA 1172220..1173920 (+) 1701 WP_150979519.1 group II intron reverse transcriptase/maturase -
  K8O84_RS05655 (K8O84_05625) ispB 1174084..1175013 (+) 930 WP_061955316.1 octaprenyl diphosphate synthase -
  K8O84_RS05665 (K8O84_05635) pilB 1175563..1177287 (+) 1725 WP_061955314.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K8O84_RS05670 (K8O84_05640) pilG 1177315..1178580 (+) 1266 WP_061955312.1 type II secretion system F family protein Machinery gene
  K8O84_RS05675 (K8O84_05645) - 1178599..1179492 (+) 894 WP_150979518.1 A24 family peptidase -
  K8O84_RS05680 (K8O84_05650) coaE 1179544..1180167 (+) 624 WP_150979517.1 dephospho-CoA kinase -
  K8O84_RS05685 (K8O84_05655) zapD 1180419..1181177 (+) 759 WP_061955303.1 cell division protein ZapD -
  K8O84_RS05690 (K8O84_05660) - 1181183..1181374 (+) 192 WP_061955301.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63216.90 Da        Isoelectric Point: 7.7874

>NTDB_id=603222 K8O84_RS05665 WP_061955314.1 1175563..1177287(+) (pilB) [Cupriavidus pauculus strain FDAARGOS_1472]
MTLGLALAQSRRIAPALLAQLEQSAREKKTQLIDEIIGTGTMSAHDVAIFAADKYQLPLLDLSQYNLNRVPPALAGNREF
HAHRLLPLGRRENRLIVAMSDPANQTGIDAIRQKFNLPVETVVVEHDKLMKHVRAAGESVGTLKNISPVTPTVQKMIEYD
PTAAAASARKGPSNDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFEFFYRVRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLISVPKEKDSKETVDKAIDFRVSTLPTLFGEKIVMRILDSSSDKLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNMLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCTCKRPGELAPQVLI
DAGFSEADIDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPISEPMQQIILSHGTALQIAEQARREGVLSLREAGLKKVKQ
GVTSLEEVLATTNT

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=603222 K8O84_RS05665 WP_061955314.1 1175563..1177287(+) (pilB) [Cupriavidus pauculus strain FDAARGOS_1472]
ATGACACTAGGCCTTGCGCTTGCCCAGAGCCGGCGGATCGCGCCAGCGCTGCTAGCTCAGCTGGAGCAGTCGGCACGGGA
AAAGAAAACGCAGTTGATCGATGAAATCATCGGCACCGGCACGATGTCGGCTCATGACGTCGCGATCTTTGCCGCGGACA
AGTATCAGTTGCCGCTGCTGGACCTGTCGCAATACAACCTTAATCGCGTGCCGCCGGCGCTGGCCGGTAATCGGGAGTTC
CACGCCCATCGCCTGCTGCCGCTGGGCCGGCGCGAAAACCGGCTGATCGTAGCGATGTCTGACCCGGCGAATCAAACCGG
CATCGACGCGATCCGCCAGAAGTTCAACCTGCCGGTGGAAACCGTCGTCGTCGAGCACGACAAGCTGATGAAGCACGTGC
GCGCGGCTGGCGAATCCGTCGGCACGCTAAAGAACATCTCGCCCGTTACGCCCACGGTGCAGAAGATGATCGAGTACGAC
CCGACAGCGGCCGCGGCAAGCGCGCGCAAGGGCCCGTCCAACGACATCGACGATGCGCCCGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAAGCCTTCCATCGCGGCGCATCGGACCTGCACTTCGAACCATTCGAATTCTTCTACCGCGTGCGTTTCC
GTGTCGACGGCGTGCTGCAGGAGGTTGCGCGGCCGCCGCTCGATATCCGCGACAAGATCGCCACGCGCATCAAGGTACTG
TCGCGGCTCGATATTTCCGAAAAGCGCGTGCCGCAAGACGGCCGGATGAAGCTGCTGATCTCGGTGCCGAAGGAAAAGGA
CAGCAAGGAAACGGTCGACAAGGCGATCGACTTCCGCGTGTCGACACTGCCTACGCTGTTCGGCGAAAAAATCGTGATGC
GGATTCTCGATTCGTCATCCGACAAGCTCGACATCGACCAGCTCGGCTACGAGCCCGAACAGAAGCAGTTGCTGCTCGAC
GTGATCAAGCGGCCGTACGGGATGGTGCTGGTCACCGGCCCGACCGGCAGCGGCAAGACCGTATCGCTCTATACGTTCCT
GAACATGCTGAATCAGGGCGACATCAATATTTCCACGGCCGAGGATCCGGCGGAAATCCAGTTGCCGGGCATCAATCAGG
TCAACGTCAACGACAAGGCCGGCCTGACCTTTGCGGCAGCGCTGAAGTCTTTCCTGCGCCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGCGATCTGGAAACGGCCGACATCTCGATCAAGGCGGCGCAGACGGGCCACCTCGTGCTGTCCACACT
GCACACCAACGATGCGCCGACCACGCTGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAATATCGCCTCGTCGGTGC
TGATGATCACCGCACAGCGGCTGGCGCGCAGGCTGTGCACGTGCAAGCGGCCCGGCGAGCTGGCGCCGCAGGTGCTGATC
GACGCCGGCTTCTCGGAGGCCGACATCGACGGCAGCTGGCAGCCTTACCATCCGGTCGGCTGCGAGCGCTGCAACGGCAG
CGGATACAAGGGACGTTGCGGCATTTATCAGGTGATGCCGATTTCGGAGCCGATGCAGCAGATCATTCTGTCGCACGGCA
CGGCGCTCCAGATAGCCGAACAAGCCCGTCGGGAGGGCGTGCTATCGTTGCGGGAAGCTGGACTCAAGAAGGTCAAGCAG
GGCGTCACGTCACTCGAAGAAGTGCTGGCGACCACAAACACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.241

98.258

0.503
  pilB Acinetobacter baylyi ADP1

51.613

97.213

0.502
  pilF Neisseria gonorrhoeae MS11

53.148

94.077

0.5
  pilB Legionella pneumophila strain ERS1305867

48.571

97.561

0.474
  pilB Vibrio cholerae strain A1552

45.061

100

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

46.31

94.425

0.437
  pilB Vibrio campbellii strain DS40M4

46.125

94.425

0.436
  pilF Thermus thermophilus HB27

37.979

100

0.38