Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   K8B76_RS09430 Genome accession   NZ_CP082820
Coordinates   1875691..1876707 (-) Length   338 a.a.
NCBI ID   WP_078102766.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain SCAID PHRX1-2021     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1870691..1881707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B76_RS10890 - 1870769..1870900 (-) 132 WP_000768904.1 hypothetical protein -
  K8B76_RS09390 (K8B76_09390) - 1870901..1872091 (-) 1191 WP_000167769.1 acetate kinase -
  K8B76_RS09395 (K8B76_09395) comYH 1872142..1873095 (-) 954 WP_000345080.1 class I SAM-dependent methyltransferase Machinery gene
  K8B76_RS09400 (K8B76_09400) - 1873156..1873750 (-) 595 Protein_1851 class I SAM-dependent methyltransferase -
  K8B76_RS09405 (K8B76_09405) comGG/cglG 1873853..1874299 (-) 447 WP_001842224.1 competence type IV pilus minor pilin ComGG Machinery gene
  K8B76_RS09410 (K8B76_09410) comGF/cglF 1874277..1874738 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  K8B76_RS09415 (K8B76_09415) comGE/cglE 1874701..1875003 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  K8B76_RS09420 (K8B76_09420) comGD/cglD 1874966..1875370 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  K8B76_RS09425 (K8B76_09425) comGC/cglC 1875363..1875689 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  K8B76_RS09430 (K8B76_09430) comGB/cglB 1875691..1876707 (-) 1017 WP_078102766.1 competence type IV pilus assembly protein ComGB Machinery gene
  K8B76_RS09435 (K8B76_09435) comGA/cglA/cilD 1876655..1877596 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  K8B76_RS09440 (K8B76_09440) - 1877672..1878037 (-) 366 WP_000286415.1 DUF1033 family protein -
  K8B76_RS09445 (K8B76_09445) - 1878188..1879246 (-) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -
  K8B76_RS09450 (K8B76_09450) nagA 1879409..1880560 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38455.45 Da        Isoelectric Point: 9.6162

>NTDB_id=602574 K8B76_RS09430 WP_078102766.1 1875691..1876707(-) (comGB/cglB) [Streptococcus pneumoniae strain SCAID PHRX1-2021]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVSRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=602574 K8B76_RS09430 WP_078102766.1 1875691..1876707(-) (comGB/cglB) [Streptococcus pneumoniae strain SCAID PHRX1-2021]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAGCCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

70.238

99.408

0.698
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503