Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NH569_RS02215 Genome accession   NZ_CP099721
Coordinates   455070..456194 (+) Length   374 a.a.
NCBI ID   WP_000228508.1    Uniprot ID   A0A0H2VB93
Organism   Escherichia coli strain EC21GDE043     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 450070..461194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH569_RS02190 (NH569_02190) mscL 450187..450600 (-) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -
  NH569_RS02195 (NH569_02195) trkA 450730..452106 (-) 1377 WP_167817978.1 Trk system potassium transporter TrkA -
  NH569_RS02200 (NH569_02200) rsmB 452128..453417 (-) 1290 WP_000744766.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  NH569_RS02205 (NH569_02205) fmt 453469..454416 (-) 948 WP_000004421.1 methionyl-tRNA formyltransferase -
  NH569_RS02210 (NH569_02210) def 454431..454940 (-) 510 WP_000114984.1 peptide deformylase -
  NH569_RS02215 (NH569_02215) dprA 455070..456194 (+) 1125 WP_000228508.1 DNA-protecting protein DprA Machinery gene
  NH569_RS02220 (NH569_02220) smg 456166..456639 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  NH569_RS02225 (NH569_02225) yrdD 456668..457210 (+) 543 WP_001312137.1 type I DNA topoisomerase -
  NH569_RS02230 (NH569_02230) tsaC 457215..457787 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  NH569_RS02235 (NH569_02235) aroE 457792..458610 (+) 819 WP_000451230.1 shikimate dehydrogenase -
  NH569_RS02240 (NH569_02240) yrdB 458607..458864 (+) 258 WP_001070567.1 DUF1488 domain-containing protein -
  NH569_RS02245 (NH569_02245) yrdA 458840..459394 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40892.82 Da        Isoelectric Point: 6.0395

>NTDB_id=602569 NH569_RS02215 WP_000228508.1 455070..456194(+) (dprA) [Escherichia coli strain EC21GDE043]
MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=602569 NH569_RS02215 WP_000228508.1 455070..456194(+) (dprA) [Escherichia coli strain EC21GDE043]
ATGGTTGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VB93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39