Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   K8B76_RS09435 Genome accession   NZ_CP082820
Coordinates   1876655..1877596 (-) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain SCAID PHRX1-2021     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1871655..1882596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B76_RS09395 (K8B76_09395) comYH 1872142..1873095 (-) 954 WP_000345080.1 class I SAM-dependent methyltransferase Machinery gene
  K8B76_RS09400 (K8B76_09400) - 1873156..1873750 (-) 595 Protein_1851 class I SAM-dependent methyltransferase -
  K8B76_RS09405 (K8B76_09405) comGG/cglG 1873853..1874299 (-) 447 WP_001842224.1 competence type IV pilus minor pilin ComGG Machinery gene
  K8B76_RS09410 (K8B76_09410) comGF/cglF 1874277..1874738 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  K8B76_RS09415 (K8B76_09415) comGE/cglE 1874701..1875003 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  K8B76_RS09420 (K8B76_09420) comGD/cglD 1874966..1875370 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  K8B76_RS09425 (K8B76_09425) comGC/cglC 1875363..1875689 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  K8B76_RS09430 (K8B76_09430) comGB/cglB 1875691..1876707 (-) 1017 WP_078102766.1 competence type IV pilus assembly protein ComGB Machinery gene
  K8B76_RS09435 (K8B76_09435) comGA/cglA/cilD 1876655..1877596 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  K8B76_RS09440 (K8B76_09440) - 1877672..1878037 (-) 366 WP_000286415.1 DUF1033 family protein -
  K8B76_RS09445 (K8B76_09445) - 1878188..1879246 (-) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -
  K8B76_RS09450 (K8B76_09450) nagA 1879409..1880560 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  K8B76_RS09455 (K8B76_09455) - 1880713..1882530 (-) 1818 WP_001220850.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=602575 K8B76_RS09435 WP_000249571.1 1876655..1877596(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain SCAID PHRX1-2021]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=602575 K8B76_RS09435 WP_000249571.1 1876655..1877596(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain SCAID PHRX1-2021]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361