Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K6745_RS12530 Genome accession   NZ_CP082303
Coordinates   2720747..2721970 (+) Length   407 a.a.
NCBI ID   WP_025768183.1    Uniprot ID   A0AA36UQ13
Organism   Vibrio alginolyticus strain FJV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2715747..2726970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6745_RS12510 (K6745_12425) ampD 2716756..2717307 (-) 552 WP_025768186.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6745_RS12515 (K6745_12430) nadC 2717400..2718287 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6745_RS12520 (K6745_12435) - 2718550..2719035 (+) 486 WP_025768185.1 pilin -
  K6745_RS12525 (K6745_12440) pilB 2719038..2720723 (+) 1686 WP_025768184.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6745_RS12530 (K6745_12445) pilC 2720747..2721970 (+) 1224 WP_025768183.1 type II secretion system F family protein Machinery gene
  K6745_RS12535 (K6745_12450) pilD 2722035..2722904 (+) 870 WP_025768182.1 prepilin peptidase Machinery gene
  K6745_RS12540 (K6745_12455) coaE 2722905..2723519 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  K6745_RS12545 (K6745_12460) zapD 2723547..2724287 (+) 741 WP_005373086.1 cell division protein ZapD -
  K6745_RS12550 (K6745_12465) yacG 2724446..2724640 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  K6745_RS12555 (K6745_12470) rplS 2724953..2725306 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  K6745_RS12560 (K6745_12475) trmD 2725348..2726109 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  K6745_RS12565 (K6745_12480) rimM 2726137..2726685 (-) 549 WP_005379964.1 ribosome maturation factor RimM -
  K6745_RS12570 (K6745_12485) rpsP 2726715..2726963 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45270.47 Da        Isoelectric Point: 10.3446

>NTDB_id=601143 K6745_RS12530 WP_025768183.1 2720747..2721970(+) (pilC) [Vibrio alginolyticus strain FJV2]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGIDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=601143 K6745_RS12530 WP_025768183.1 2720747..2721970(+) (pilC) [Vibrio alginolyticus strain FJV2]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCTGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCATAGACATCACGGTGTTTACCCGTCAGATTTCGACGATGTTG
ATAACCGGTGTACCCTTAGTTCAGGCTTTAAAATTGGTCTCAGAAAATCATAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCGATGGTAGTGCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACAAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCGTGGTTTACCAGGC
AAGTATTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATTTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGATCTGTGCTTAAATTTCCTGTCCTGGGCCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGCATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACATCAGGAAACTTGCACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTAGCCATGCGCAACTGTAATGTGTTCCCTGAATTGGTGTTACAAATGGTTATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACAATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTTTTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

87.224

100

0.872
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilC Legionella pneumophila strain ERS1305867

40.295

100

0.403
  pilG Neisseria gonorrhoeae MS11

40.299

98.771

0.398
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilG Neisseria meningitidis 44/76-A

40.05

98.771

0.396