Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6745_RS12525 Genome accession   NZ_CP082303
Coordinates   2719038..2720723 (+) Length   561 a.a.
NCBI ID   WP_025768184.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FJV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2714038..2725723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6745_RS12505 (K6745_12420) pdhR 2715583..2716350 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  K6745_RS12510 (K6745_12425) ampD 2716756..2717307 (-) 552 WP_025768186.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6745_RS12515 (K6745_12430) nadC 2717400..2718287 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6745_RS12520 (K6745_12435) - 2718550..2719035 (+) 486 WP_025768185.1 pilin -
  K6745_RS12525 (K6745_12440) pilB 2719038..2720723 (+) 1686 WP_025768184.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6745_RS12530 (K6745_12445) pilC 2720747..2721970 (+) 1224 WP_025768183.1 type II secretion system F family protein Machinery gene
  K6745_RS12535 (K6745_12450) pilD 2722035..2722904 (+) 870 WP_025768182.1 prepilin peptidase Machinery gene
  K6745_RS12540 (K6745_12455) coaE 2722905..2723519 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  K6745_RS12545 (K6745_12460) zapD 2723547..2724287 (+) 741 WP_005373086.1 cell division protein ZapD -
  K6745_RS12550 (K6745_12465) yacG 2724446..2724640 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  K6745_RS12555 (K6745_12470) rplS 2724953..2725306 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62089.92 Da        Isoelectric Point: 5.5335

>NTDB_id=601142 K6745_RS12525 WP_025768184.1 2719038..2720723(+) (pilB) [Vibrio alginolyticus strain FJV2]
MLTNLSTVLRQAGILSFSQEESLLEQAKASGISMPEALLSCGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLTLAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLNLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=601142 K6745_RS12525 WP_025768184.1 2719038..2720723(+) (pilB) [Vibrio alginolyticus strain FJV2]
ATGCTAACCAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGTCTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
TAAAGCTTCTGGCATTTCAATGCCGGAGGCTTTGCTCAGTTGTGGCTTTTTCCAATCACATGAGCTGACTGAGCACCTTA
GCTCTATCTTTGGACTTTCCTGCACATCACTCAGTCAGTATGAGTACTCGTCGCTGTGCCAAAAGCTTGGCTTGCGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCCACATTAACACTCGCGGTTGCAGACCCAACTAACTT
ACAAGCGGAAGATGACTTTCGTTTTGCGACAGGGTTGCAAGTAGAGCTTGTTCTCGCTGATTTTCGCGAGCTGACCGCTG
CGATTCGGCGCTTATATGGCCGAGCACTTGGGCAAGAAAAGTCTGGATTAAAAGAGATCAACCAAGAAGAATTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGACGAATCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGTGTGCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAACTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGGCTGAGTATCCTCAATAAGCCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCGGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAATTTGATTATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGAATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCAGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAGATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

92.692

100

0.927
  pilB Vibrio parahaemolyticus RIMD 2210633

92.692

100

0.927
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Acinetobacter baumannii D1279779

52.115

92.692

0.483
  pilB Acinetobacter baylyi ADP1

47.849

99.465

0.476
  pilB Legionella pneumophila strain ERS1305867

48.803

96.791

0.472
  pilF Neisseria gonorrhoeae MS11

43.794

100

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

36.634

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.494

99.643

0.364
  pilF Thermus thermophilus HB27

36.299

100

0.364