Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K5K89_RS04965 Genome accession   NZ_CP081944
Coordinates   1138716..1139924 (-) Length   402 a.a.
NCBI ID   WP_222540967.1    Uniprot ID   -
Organism   Pseudomonas sp. DR208     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1133716..1144924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5K89_RS04925 (K5K89_04925) - 1133748..1135046 (-) 1299 WP_071491077.1 NAD(P)/FAD-dependent oxidoreductase -
  K5K89_RS04930 (K5K89_04930) - 1135107..1135280 (-) 174 WP_046034083.1 DUF3094 family protein -
  K5K89_RS04935 (K5K89_04935) - 1135337..1135966 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  K5K89_RS04940 (K5K89_04940) - 1136068..1136757 (+) 690 WP_222540964.1 energy-coupling factor ABC transporter permease -
  K5K89_RS04945 (K5K89_04945) - 1136824..1137039 (+) 216 WP_003171680.1 hypothetical protein -
  K5K89_RS04950 (K5K89_04950) yacG 1137036..1137236 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  K5K89_RS04955 (K5K89_04955) coaE 1137233..1137856 (-) 624 WP_222540965.1 dephospho-CoA kinase -
  K5K89_RS04960 (K5K89_04960) pilD 1137853..1138719 (-) 867 WP_222540966.1 prepilin peptidase Machinery gene
  K5K89_RS04965 (K5K89_04965) pilC 1138716..1139924 (-) 1209 WP_222540967.1 type II secretion system F family protein Machinery gene
  K5K89_RS04970 (K5K89_04970) - 1140118..1140540 (+) 423 WP_092240655.1 pilin -
  K5K89_RS04975 (K5K89_04975) - 1140576..1141976 (+) 1401 WP_222540968.1 O-antigen ligase family protein -
  K5K89_RS04985 (K5K89_04985) - 1142242..1142547 (+) 306 WP_033903347.1 DUF6388 family protein -
  K5K89_RS04990 (K5K89_04990) nadC 1142595..1143443 (-) 849 WP_071491085.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43162.45 Da        Isoelectric Point: 9.6882

>NTDB_id=598608 K5K89_RS04965 WP_222540967.1 1138716..1139924(-) (pilC) [Pseudomonas sp. DR208]
MDNASTIYAWEGINRKGRRVSGHSTGHNPALVKAQLRRQGISPGLVRKHSPVLAGLKPSIKSADITQLTRQLATLLKAGI
PLLQALDIISEGFDNATLRELLQGLQQSIAAGTSLADALRKHPRCFDELYCNLVAAGEQAGALETLLERVAIHREKSEQL
KARIKKAMTYPITVLIVASLVTGVLLVHVVPQFQSLFAGVDGKLPGFTLQVIALSEFMQRAWWGVALGLLAAGFGLRHAY
HALPAFQLWLDAGLLKVPVAGKLLRKSAVARYARTLSTTFAAGVPLVQALDSVAGAAGSEPFRQAIEHMRHDVSTGMQLN
QSMAVSGLFPGMAIQMTAIGEESGTLDRMLEKVANQYEADVDTLVDHLTSLMEPLIMVILGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=598608 K5K89_RS04965 WP_222540967.1 1138716..1139924(-) (pilC) [Pseudomonas sp. DR208]
ATGGACAACGCTTCAACGATCTACGCTTGGGAAGGCATCAACCGCAAAGGGCGCAGGGTGTCCGGGCACAGCACCGGGCA
CAACCCTGCCCTGGTCAAGGCTCAGCTGCGCCGACAAGGCATCAGCCCAGGGCTTGTACGCAAGCATTCTCCAGTTCTAG
CGGGCCTCAAGCCATCTATCAAATCAGCGGATATCACCCAGCTCACGCGCCAACTGGCAACCTTGCTGAAGGCCGGGATT
CCTCTGCTGCAAGCCCTCGACATCATCAGCGAAGGCTTTGACAACGCTACGCTGCGCGAGCTGCTACAGGGGTTGCAACA
GTCAATCGCCGCTGGCACCAGCCTTGCCGACGCGTTGCGCAAGCATCCGCGCTGTTTCGATGAGCTGTATTGCAACCTGG
TGGCGGCCGGCGAACAGGCCGGTGCCCTTGAAACCTTGCTGGAGCGCGTAGCGATTCATAGGGAAAAGAGCGAGCAGTTA
AAGGCCAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGATAGTCGCCAGCCTGGTCACCGGCGTGCTGCTGGT
CCACGTAGTGCCGCAGTTCCAAAGCCTGTTCGCCGGAGTCGACGGCAAACTGCCCGGCTTCACCTTGCAGGTGATCGCTT
TGTCCGAGTTCATGCAGCGAGCCTGGTGGGGCGTGGCACTGGGCCTGCTTGCCGCGGGTTTTGGGTTGCGCCACGCCTAC
CATGCCTTACCGGCTTTCCAGCTGTGGCTGGATGCCGGTTTGTTGAAAGTGCCCGTGGCAGGCAAACTGCTGAGAAAATC
GGCCGTGGCCCGCTACGCCCGCACGCTGTCCACTACCTTCGCCGCCGGTGTTCCGTTGGTGCAGGCCCTGGACTCGGTGG
CCGGGGCCGCCGGTTCTGAGCCGTTCAGACAGGCAATCGAGCATATGCGACACGACGTATCCACTGGCATGCAACTGAAT
CAATCCATGGCCGTCAGCGGCCTGTTTCCCGGCATGGCCATCCAGATGACGGCCATCGGCGAAGAGTCGGGCACGCTTGA
CCGCATGCTGGAGAAGGTCGCCAACCAGTACGAGGCCGATGTGGACACCCTGGTCGATCACCTCACTAGCCTGATGGAGC
CTCTGATCATGGTGATACTGGGGGGCATCGTCGGCGCGCTGGTGATTGCCATGTACCTGCCGGTATTCCAACTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.69

98.756

0.58
  pilC Acinetobacter baylyi ADP1

51.515

98.507

0.507
  pilC Acinetobacter baumannii D1279779

51.139

98.259

0.502
  pilC Legionella pneumophila strain ERS1305867

48.608

98.259

0.478
  pilC Vibrio cholerae strain A1552

41.895

99.751

0.418
  pilG Neisseria gonorrhoeae MS11

41.299

95.771

0.396
  pilC Vibrio campbellii strain DS40M4

39.599

99.254

0.393
  pilG Neisseria meningitidis 44/76-A

40.779

95.771

0.391
  pilC Thermus thermophilus HB27

36.591

99.254

0.363