Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K5F93_RS04730 Genome accession   NZ_CP081490
Coordinates   997479..998696 (-) Length   405 a.a.
NCBI ID   WP_221810777.1    Uniprot ID   -
Organism   Pseudomonas protegens strain PS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 992479..1003696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5F93_RS04690 (K5F93_04690) - 993196..993369 (-) 174 WP_221810773.1 DUF3094 family protein -
  K5F93_RS04695 (K5F93_04695) - 993558..994187 (-) 630 WP_102882240.1 DUF1780 domain-containing protein -
  K5F93_RS04700 (K5F93_04700) - 994332..994799 (+) 468 WP_102882239.1 FAD/FMN-containing dehydrogenase -
  K5F93_RS04705 (K5F93_04705) - 994796..995485 (+) 690 WP_102882238.1 energy-coupling factor ABC transporter permease -
  K5F93_RS04710 (K5F93_04710) - 995549..995764 (+) 216 WP_102865473.1 hypothetical protein -
  K5F93_RS04715 (K5F93_04715) yacG 995784..995987 (-) 204 WP_221810774.1 DNA gyrase inhibitor YacG -
  K5F93_RS04720 (K5F93_04720) coaE 995984..996607 (-) 624 WP_221810775.1 dephospho-CoA kinase -
  K5F93_RS04725 (K5F93_04725) pilD 996604..997476 (-) 873 WP_221810776.1 prepilin peptidase Machinery gene
  K5F93_RS04730 (K5F93_04730) pilC 997479..998696 (-) 1218 WP_221810777.1 type II secretion system F family protein Machinery gene
  K5F93_RS04735 (K5F93_04735) - 998922..999338 (+) 417 WP_221810778.1 pilin -
  K5F93_RS04740 (K5F93_04740) - 999577..999933 (+) 357 WP_221810779.1 BON domain-containing protein -
  K5F93_RS04745 (K5F93_04745) - 1000120..1003038 (-) 2919 WP_221810780.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44285.02 Da        Isoelectric Point: 10.7002

>NTDB_id=596956 K5F93_RS04730 WP_221810777.1 997479..998696(-) (pilC) [Pseudomonas protegens strain PS1]
MAVKAPVASVYLWQGTDGQGHTLAGELSAANPALIRAQLRRQGITPTRVRRKPRAWLRSGQRIKAREIALFTRQLATLMQ
AGVPLLQAFDIIAESFVQPLMRQLLASLKRDIEAGSSLATALRRHPRHFDELYCNLVEAGEQSGALDTLLERLATYREKS
ENLKARVKKAMVYPVAVIGVAVLVSSILLIKVVPQFQSLFAGFNAQLPLLTQWVIALSHVIQNHGGWLLSAGLLMGVGIR
QAYRRSERLRDRVDQGLLRLPLAGPLLYKAAVARYARTLATTFAAGVPLVQALDSVAGASGNKVFRQAVERIRQQVASGM
QLNFAMRASGVFPTMAIQMTAIGEESGMLDHLLEKVAAHYEVEVDHLVDSLTSLMEPLIMVILGSIVGALVFAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=596956 K5F93_RS04730 WP_221810777.1 997479..998696(-) (pilC) [Pseudomonas protegens strain PS1]
ATGGCGGTAAAAGCACCTGTGGCCAGCGTCTATCTCTGGCAAGGCACCGACGGCCAGGGCCACACGTTGGCCGGCGAGCT
GAGCGCTGCAAACCCGGCGCTGATCCGGGCTCAGCTGCGCAGGCAAGGCATCACTCCAACCCGGGTACGCCGCAAGCCCA
GGGCCTGGCTCCGATCGGGCCAACGAATCAAGGCACGGGAGATCGCCCTGTTCACCCGGCAACTGGCAACCCTGATGCAG
GCCGGCGTGCCACTGCTGCAAGCCTTCGACATCATTGCCGAGAGCTTCGTCCAGCCGTTGATGCGCCAACTGCTGGCCAG
CCTCAAACGCGACATCGAGGCTGGCTCCAGCCTGGCCACGGCGCTGCGCCGACATCCGCGCCACTTTGACGAGCTGTACT
GCAACCTGGTGGAGGCCGGTGAACAGTCCGGAGCCCTGGATACCCTGCTGGAACGGCTCGCCACCTATAGGGAAAAAAGC
GAGAACCTCAAGGCCCGGGTAAAAAAGGCCATGGTCTATCCCGTGGCGGTGATCGGCGTGGCCGTCCTGGTCAGCAGCAT
CCTGCTGATCAAGGTGGTTCCCCAATTCCAGAGCCTGTTCGCCGGGTTCAACGCCCAATTGCCGCTACTGACCCAATGGG
TCATCGCCCTTTCCCATGTCATCCAGAACCACGGCGGATGGTTGCTCAGCGCCGGCCTGCTGATGGGGGTAGGGATCAGG
CAGGCTTATCGGCGCTCGGAGCGCCTGCGGGACCGTGTGGATCAGGGACTGCTACGCCTGCCCTTGGCCGGCCCGCTGCT
GTACAAGGCCGCAGTGGCCCGCTACGCCCGTACGCTGGCCACCACCTTCGCGGCTGGCGTGCCTCTGGTCCAGGCCCTGG
ATTCAGTGGCAGGAGCCAGCGGCAACAAGGTCTTCAGGCAAGCGGTGGAACGCATCCGCCAACAGGTGGCCAGTGGCATG
CAACTGAACTTTGCCATGCGCGCCAGCGGCGTCTTCCCTACTATGGCGATCCAGATGACCGCCATCGGTGAAGAATCCGG
AATGCTGGATCATCTGCTGGAAAAAGTCGCAGCGCATTATGAGGTCGAGGTGGACCACCTGGTCGACAGCCTGACCAGCC
TCATGGAGCCGTTGATCATGGTCATCCTGGGCAGTATCGTCGGCGCCCTGGTGTTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.272

100

0.583
  pilC Acinetobacter baylyi ADP1

54.034

100

0.546
  pilC Acinetobacter baumannii D1279779

52.696

100

0.531
  pilC Legionella pneumophila strain ERS1305867

48.411

100

0.489
  pilC Vibrio campbellii strain DS40M4

42.04

99.259

0.417
  pilC Vibrio cholerae strain A1552

42.857

96.79

0.415
  pilG Neisseria meningitidis 44/76-A

42.105

98.519

0.415
  pilG Neisseria gonorrhoeae MS11

42.317

98.025

0.415
  pilC Thermus thermophilus HB27

38.5

98.765

0.38