Detailed information NTG0596955

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: K5F93_RS04725
• Genome accession: NZ_CP081490
• Coordinates: 996604..997476 (-)
• Length: 290 a.a.
• NCBI ID: WP_221810776.1
• Uniprot ID: -
• Organism: Pseudomonas protegens strain PS1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 991604..1002476

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K5F93_RS04685 (K5F93_04685)	-	991837..993135 (-)	1299	WP_221810772.1	NAD(P)/FAD-dependent oxidoreductase	-
K5F93_RS04690 (K5F93_04690)	-	993196..993369 (-)	174	WP_221810773.1	DUF3094 family protein	-
K5F93_RS04695 (K5F93_04695)	-	993558..994187 (-)	630	WP_102882240.1	DUF1780 domain-containing protein	-
K5F93_RS04700 (K5F93_04700)	-	994332..994799 (+)	468	WP_102882239.1	FAD/FMN-containing dehydrogenase	-
K5F93_RS04705 (K5F93_04705)	-	994796..995485 (+)	690	WP_102882238.1	energy-coupling factor ABC transporter permease	-
K5F93_RS04710 (K5F93_04710)	-	995549..995764 (+)	216	WP_102865473.1	hypothetical protein	-
K5F93_RS04715 (K5F93_04715)	yacG	995784..995987 (-)	204	WP_221810774.1	DNA gyrase inhibitor YacG	-
K5F93_RS04720 (K5F93_04720)	coaE	995984..996607 (-)	624	WP_221810775.1	dephospho-CoA kinase	-
K5F93_RS04725 (K5F93_04725)	pilD	996604..997476 (-)	873	WP_221810776.1	prepilin peptidase	Machinery gene
K5F93_RS04730 (K5F93_04730)	pilC	997479..998696 (-)	1218	WP_221810777.1	type II secretion system F family protein	Machinery gene
K5F93_RS04735 (K5F93_04735)	-	998922..999338 (+)	417	WP_221810778.1	pilin	-
K5F93_RS04740 (K5F93_04740)	-	999577..999933 (+)	357	WP_221810779.1	BON domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32109.34 Da
• Isoelectric Point: 7.0714

>NTDB_id=596955 K5F93_RS04725 WP_221810776.1 996604..997476(-) (pilD) [Pseudomonas protegens strain PS1]
MSLTEVMTLYPLAFVTAALVLGLLVGSFLNVLVWRLPKMLERDWRAQAHEVLGLPPEPVAAPYNLLLPHSQCPHCAQPIR
PWENIPLLSYLWLRGRCAHCRTAISPRYPLTELACGLISAYVAWHFGFGWPAGLLLVLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANSFEVLVSLEDALWGAVLGYLCLWSVFWLFKLLTGKDGMGHGDFKLLAMLGAWGGWQILPLTLLLSSLVG
AILGTVMLRLNRAKTSTPIPFGPFLAIAGWIALLWGGQMTDFYLQFVGFQ

Nucleotide

• Length: 873 bp

>NTDB_id=596955 K5F93_RS04725 WP_221810776.1 996604..997476(-) (pilD) [Pseudomonas protegens strain PS1]
ATGTCCTTGACTGAAGTCATGACCCTTTACCCCTTGGCCTTCGTGACTGCGGCCCTGGTACTGGGGCTGCTCGTAGGCAG
CTTTCTGAACGTGCTGGTGTGGCGCCTGCCGAAAATGCTTGAACGCGATTGGCGGGCTCAAGCCCACGAAGTGCTGGGAC
TGCCGCCGGAGCCTGTGGCGGCGCCCTACAACCTGCTGCTGCCCCACTCCCAGTGCCCCCATTGCGCACAACCGATCCGC
CCCTGGGAAAATATTCCCCTGCTCAGCTATCTGTGGCTGCGGGGCCGTTGCGCCCACTGCAGGACCGCCATCAGCCCGCG
CTACCCACTAACCGAGCTGGCTTGCGGGCTGATCTCGGCGTACGTCGCCTGGCACTTCGGTTTCGGCTGGCCCGCGGGCC
TGCTGCTGGTCCTCAGTTGGGGGCTACTGGCCATGAGCCTGATCGATGCCGATCACCAACTGCTCCCAGACGTGCTGGTG
TTGCCACTGCTGTGGCTGGGACTGATTGCCAACAGCTTCGAGGTACTGGTGAGCCTGGAGGATGCCCTGTGGGGCGCGGT
ACTCGGCTATCTGTGCCTGTGGTCTGTGTTCTGGCTGTTCAAATTGCTGACCGGCAAGGACGGCATGGGCCACGGCGACT
TCAAGCTGCTAGCAATGCTTGGCGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCTTGCTCCTGTCCTCTCTGGTGGGA
GCGATTCTCGGCACTGTCATGCTGCGCCTGAACCGGGCCAAGACCTCGACGCCCATCCCCTTCGGCCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATGACCGACTTCTATTTGCAGTTTGTCGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.386	98.276	0.534
pilD	Vibrio campbellii strain DS40M4	50	97.241	0.486
pilD	Acinetobacter baumannii D1279779	49.635	94.483	0.469
pilD	Acinetobacter nosocomialis M2	49.635	94.483	0.469
pilD	Neisseria gonorrhoeae MS11	48.889	93.103	0.455