Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   K5K97_RS09340 Genome accession   NZ_CP081470
Coordinates   1811582..1812049 (+) Length   155 a.a.
NCBI ID   WP_227288560.1    Uniprot ID   -
Organism   Vagococcus fluvialis strain 25B2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1800949..1840609 1811582..1812049 within 0


Gene organization within MGE regions


Location: 1800949..1840609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5K97_RS09245 (K5K97_09240) - 1800949..1802070 (-) 1122 WP_227288542.1 tyrosine-type recombinase/integrase -
  K5K97_RS09250 (K5K97_09245) - 1802170..1802598 (-) 429 WP_227288543.1 hypothetical protein -
  K5K97_RS09255 (K5K97_09250) - 1802688..1803317 (-) 630 WP_227288544.1 LexA family transcriptional regulator -
  K5K97_RS09260 (K5K97_09255) - 1803519..1803755 (+) 237 WP_227288545.1 DUF739 family protein -
  K5K97_RS09265 (K5K97_09260) - 1803767..1804663 (+) 897 WP_227288546.1 DUF3102 domain-containing protein -
  K5K97_RS09270 (K5K97_09265) - 1804656..1805123 (+) 468 WP_227288547.1 ORF6C domain-containing protein -
  K5K97_RS09275 (K5K97_09270) - 1805136..1805453 (+) 318 WP_207058582.1 DUF771 domain-containing protein -
  K5K97_RS09280 (K5K97_09275) - 1805608..1805766 (+) 159 WP_227288548.1 hypothetical protein -
  K5K97_RS09285 (K5K97_09280) - 1805763..1806434 (+) 672 WP_227288549.1 N-6 DNA methylase -
  K5K97_RS09290 (K5K97_09285) - 1806435..1807376 (+) 942 WP_227288550.1 site-specific integrase -
  K5K97_RS09295 (K5K97_09290) - 1807387..1807623 (+) 237 WP_227288551.1 hypothetical protein -
  K5K97_RS09300 (K5K97_09295) - 1807625..1808206 (+) 582 WP_227288552.1 HNH endonuclease signature motif containing protein -
  K5K97_RS09305 (K5K97_09300) - 1808209..1808580 (+) 372 WP_227288553.1 hypothetical protein -
  K5K97_RS09310 (K5K97_09305) - 1808594..1808950 (+) 357 WP_227288554.1 YopX family protein -
  K5K97_RS09315 (K5K97_09310) - 1809029..1809334 (+) 306 WP_227288555.1 hypothetical protein -
  K5K97_RS09320 (K5K97_09315) - 1809351..1809896 (+) 546 WP_227288556.1 host-nuclease inhibitor Gam family protein -
  K5K97_RS09325 (K5K97_09320) - 1809906..1810544 (+) 639 WP_227288557.1 ERF family protein -
  K5K97_RS09330 (K5K97_09325) - 1810549..1811250 (+) 702 WP_227288558.1 putative HNHc nuclease -
  K5K97_RS09335 (K5K97_09330) - 1811247..1811582 (+) 336 WP_227288559.1 hypothetical protein -
  K5K97_RS09340 (K5K97_09335) ssb 1811582..1812049 (+) 468 WP_227288560.1 single-stranded DNA-binding protein Machinery gene
  K5K97_RS09345 (K5K97_09340) - 1812064..1812849 (+) 786 WP_227288561.1 phage replisome organizer N-terminal domain-containing protein -
  K5K97_RS09350 (K5K97_09345) - 1812849..1813766 (+) 918 WP_227288562.1 DnaA ATPase domain-containing protein -
  K5K97_RS09355 (K5K97_09350) - 1813766..1813948 (+) 183 WP_227288563.1 hypothetical protein -
  K5K97_RS09360 (K5K97_09355) - 1813926..1814267 (+) 342 WP_227288564.1 hypothetical protein -
  K5K97_RS09365 (K5K97_09360) - 1814270..1814638 (+) 369 WP_227289695.1 DUF1064 domain-containing protein -
  K5K97_RS09370 (K5K97_09365) - 1814650..1815459 (+) 810 WP_227288565.1 site-specific DNA-methyltransferase -
  K5K97_RS09375 (K5K97_09370) - 1815472..1815633 (+) 162 WP_227288566.1 hypothetical protein -
  K5K97_RS09380 (K5K97_09375) - 1815646..1816329 (+) 684 WP_227288567.1 helix-turn-helix transcriptional regulator -
  K5K97_RS09385 (K5K97_09380) - 1816467..1816622 (+) 156 WP_227288568.1 hypothetical protein -
  K5K97_RS09390 (K5K97_09385) - 1816755..1816991 (+) 237 WP_227288569.1 hypothetical protein -
  K5K97_RS09395 (K5K97_09390) - 1816995..1817408 (+) 414 WP_227288570.1 integrase -
  K5K97_RS09400 (K5K97_09395) - 1817709..1818047 (+) 339 WP_227288571.1 hypothetical protein -
  K5K97_RS09405 (K5K97_09400) - 1818363..1818590 (+) 228 WP_227288572.1 hypothetical protein -
  K5K97_RS09410 (K5K97_09405) - 1818712..1819008 (+) 297 WP_227289697.1 HNH endonuclease -
  K5K97_RS09415 (K5K97_09410) - 1819103..1819465 (+) 363 WP_227288573.1 hypothetical protein -
  K5K97_RS09420 (K5K97_09415) - 1819458..1821152 (+) 1695 WP_227288574.1 terminase large subunit -
  K5K97_RS09425 (K5K97_09420) - 1821298..1822341 (+) 1044 WP_227289699.1 phage portal protein -
  K5K97_RS09430 (K5K97_09425) - 1822322..1822993 (+) 672 WP_227288575.1 head maturation protease, ClpP-related -
  K5K97_RS09435 (K5K97_09430) - 1822993..1824135 (+) 1143 WP_227288576.1 phage major capsid protein -
  K5K97_RS09440 (K5K97_09435) - 1824277..1824534 (+) 258 WP_227288577.1 head-tail connector protein -
  K5K97_RS09445 (K5K97_09440) - 1824518..1824832 (+) 315 WP_227288578.1 phage head closure protein -
  K5K97_RS09450 (K5K97_09445) - 1824822..1825157 (+) 336 WP_227288579.1 hypothetical protein -
  K5K97_RS09455 (K5K97_09450) - 1825150..1825515 (+) 366 WP_227288580.1 HK97-gp10 family putative phage morphogenesis protein -
  K5K97_RS09460 (K5K97_09455) - 1825522..1826145 (+) 624 WP_227288581.1 major tail protein -
  K5K97_RS09465 (K5K97_09460) - 1826145..1826615 (+) 471 WP_227288582.1 hypothetical protein -
  K5K97_RS09470 (K5K97_09465) - 1826807..1829116 (+) 2310 WP_227288583.1 phage tail tape measure protein -
  K5K97_RS09475 (K5K97_09470) - 1829116..1829832 (+) 717 WP_227288584.1 phage tail protein -
  K5K97_RS09480 (K5K97_09475) - 1829829..1832495 (+) 2667 WP_227288585.1 phage tail tip lysozyme -
  K5K97_RS09485 (K5K97_09480) - 1832508..1834673 (+) 2166 WP_227288586.1 BppU family phage baseplate upper protein -
  K5K97_RS09490 (K5K97_09485) - 1834778..1835071 (+) 294 WP_227288587.1 hypothetical protein -
  K5K97_RS09495 (K5K97_09490) - 1835071..1835268 (+) 198 WP_227288588.1 phage holin -
  K5K97_RS14200 (K5K97_09495) - 1835322..1836158 (+) 837 WP_319796276.1 SH3 domain-containing protein -
  K5K97_RS09510 (K5K97_09500) - 1836241..1836519 (-) 279 WP_227288589.1 hypothetical protein -
  K5K97_RS09515 (K5K97_09505) - 1836986..1837234 (+) 249 WP_227288590.1 hypothetical protein -
  K5K97_RS09520 (K5K97_09510) - 1837352..1837579 (+) 228 WP_227288591.1 helix-turn-helix domain-containing protein -
  K5K97_RS09525 (K5K97_09515) - 1837560..1837910 (+) 351 WP_227288592.1 hypothetical protein -
  K5K97_RS09530 (K5K97_09520) - 1838022..1838897 (+) 876 WP_167806177.1 polysaccharide deacetylase family protein -
  K5K97_RS09535 (K5K97_09525) - 1839206..1840609 (+) 1404 WP_167799927.1 PTS sugar transporter subunit IIC -

Sequence


Protein


Download         Length: 155 a.a.        Molecular weight: 17423.25 Da        Isoelectric Point: 5.2337

>NTDB_id=596673 K5K97_RS09340 WP_227288560.1 1811582..1812049(+) (ssb) [Vagococcus fluvialis strain 25B2]
MINNVVLVGRLTRDPELKFTNNGSAVATFNLAVNRNFTNASGEREADFVNCVIWRKPAETLANYAKKGTLIGAVGRIQTR
NYENQEGRKVYVTEVVCDNFQLLESKKDNNQNNQQNNQSFHQDSMPGMDKRGFNNATDPFGQSSQIDISDDDLPF

Nucleotide


Download         Length: 468 bp        

>NTDB_id=596673 K5K97_RS09340 WP_227288560.1 1811582..1812049(+) (ssb) [Vagococcus fluvialis strain 25B2]
ATGATTAACAACGTCGTACTAGTTGGTCGCTTAACTCGTGACCCAGAACTTAAATTTACTAACAATGGTTCGGCAGTGGC
AACTTTCAATCTAGCTGTTAACCGTAATTTTACAAATGCAAGTGGTGAGCGTGAAGCTGATTTTGTTAACTGCGTTATTT
GGAGGAAGCCTGCAGAAACTTTAGCTAATTATGCTAAGAAAGGTACTTTGATTGGTGCAGTCGGACGTATTCAGACGCGT
AACTATGAAAATCAGGAAGGAAGAAAAGTTTACGTCACAGAAGTAGTATGCGATAACTTCCAGTTATTAGAGTCTAAAAA
AGATAACAATCAGAATAACCAACAAAATAACCAATCGTTCCATCAAGATAGTATGCCAGGAATGGATAAAAGAGGTTTTA
ACAATGCTACTGATCCATTCGGACAAAGTAGTCAGATTGATATTTCAGATGACGATTTGCCGTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.619

  ssbA Bacillus subtilis subsp. subtilis str. 168

51.892

100

0.619

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.377

68.387

0.413

  ssbB/cilA Streptococcus pneumoniae TIGR4

46.565

84.516

0.394

  ssbB/cilA Streptococcus pneumoniae Rx1

45.802

84.516

0.387

  ssbB/cilA Streptococcus pneumoniae D39

45.802

84.516

0.387

  ssbB/cilA Streptococcus pneumoniae R6

45.802

84.516

0.387

  ssbB/cilA Streptococcus mitis NCTC 12261

45.802

84.516

0.387

  ssbB/cilA Streptococcus mitis SK321

45.802

84.516

0.387

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.511

84.516

0.368