Detailed information NTG0593044

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: K1T74_RS02460
• Genome accession: NZ_CP080462
• Coordinates: 510553..512241 (-)
• Length: 562 a.a.
• NCBI ID: WP_220105996.1
• Uniprot ID: -
• Organism: Vibrio cholerae strain ICDC-VC702
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	512248..528416	510553..512241	flank	7

Gene organization within MGE regions

Location: 510553..528416

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K1T74_RS02460	pilB	510553..512241 (-)	1689	WP_220105996.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K1T74_RS02465	pilA	512248..512646 (-)	399	WP_142732788.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
K1T74_RS02470	nadC	512891..513781 (-)	891	WP_000665276.1	carboxylating nicotinate-nucleotide diphosphorylase	-
K1T74_RS02475	ampD	514077..514622 (+)	546	WP_088128396.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
K1T74_RS02480	fldB	514772..515290 (-)	519	WP_000690112.1	flavodoxin FldB	-
K1T74_RS02485	xerD	515428..516336 (+)	909	WP_001287472.1	site-specific tyrosine recombinase XerD	-
K1T74_RS02490	dsbC	516353..517105 (+)	753	WP_000115052.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
K1T74_RS02495	recJ	517164..518903 (+)	1740	WP_220105997.1	single-stranded-DNA-specific exonuclease RecJ	-
K1T74_RS02500	-	518948..520852 (-)	1905	WP_182293216.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
K1T74_RS02505	pdhR	521336..522106 (+)	771	WP_000331318.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
K1T74_RS02510	aceE	522156..524816 (+)	2661	WP_001281965.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
K1T74_RS02515	aceF	524835..526721 (+)	1887	WP_220105998.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
K1T74_RS02520	lpdA	526989..528416 (+)	1428	WP_000031534.1	dihydrolipoyl dehydrogenase	-

Sequence

Protein

• Length: 562 a.a.
• Molecular weight: 62056.08 Da
• Isoelectric Point: 5.8691

>NTDB_id=593044 K1T74_RS02460 WP_220105996.1 510553..512241(-) (pilB) [Vibrio cholerae strain ICDC-VC702]
MITNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQNQFAFQPDE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRATIHHIESLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide

• Length: 1689 bp

>NTDB_id=593044 K1T74_RS02460 WP_220105996.1 510553..512241(-) (pilB) [Vibrio cholerae strain ICDC-VC702]
ATGATCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGGACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTGACCGAGCAACTGA
GCCATATTTTCGGTTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTTGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTTCTGGCGGTCTCTGACCCAACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGGCTGCAAGTTGAACTGGCACTGGCCGATCACCGTGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCGGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTACTGCTCGATGCGGTGCGCAAAGGCGCATCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGTATCCGCC
TGCGCTGTGATGGCATTTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGTGTATCCACGCTTCCTACGTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCTGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTAATGACAGGCCCAACCGGCAGCGGCAAAACCGTTTCACTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAACCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCATTTCTACGTCAAGACCCGGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCATACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAATCTGGCGTCATCACTCAGTTTAATCATCGCCCAGCGTCTTGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCTCTATTGCAAAACCAATTTGCATTTCAGCCGGATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCACCATTCATCACATTGAGAGCTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTACAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (40-141)

• Type II/IV secretion system protein (T2SSE) (188-455)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio cholerae strain A1552	98.399	100	0.984
pilB	Vibrio parahaemolyticus RIMD 2210633	73.665	100	0.737
pilB	Vibrio campbellii strain DS40M4	73.31	100	0.733
pilB	Legionella pneumophila strain ERS1305867	52.838	90.925	0.48
pilB	Acinetobacter baylyi ADP1	50.385	92.527	0.466
pilB	Acinetobacter baumannii D1279779	50.385	92.527	0.466
pilF	Neisseria gonorrhoeae MS11	45.907	100	0.459
pilB/pilB1	Synechocystis sp. PCC 6803	36.833	100	0.393
pilF	Thermus thermophilus HB27	39.483	96.441	0.381
pilB	Haemophilus influenzae 86-028NP	39.658	93.772	0.372
pilB	Haemophilus influenzae Rd KW20	39.197	93.06	0.365