Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0H61_RS05105 Genome accession   NZ_CP080413
Coordinates   1137461..1138573 (+) Length   370 a.a.
NCBI ID   WP_220051659.1    Uniprot ID   -
Organism   Shewanella acanthi strain FJAT-51860     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1132461..1143573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H61_RS05070 (K0H61_05070) rdgB 1132502..1133119 (-) 618 WP_220051653.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0H61_RS05075 (K0H61_05075) - 1133232..1133666 (-) 435 WP_220051654.1 DUF4426 domain-containing protein -
  K0H61_RS05080 (K0H61_05080) yggU 1133751..1134041 (-) 291 WP_220051655.1 DUF167 family protein YggU -
  K0H61_RS05085 (K0H61_05085) - 1134041..1134589 (-) 549 WP_220051656.1 YggT family protein -
  K0H61_RS05090 (K0H61_05090) proC 1134630..1135448 (-) 819 WP_220051657.1 pyrroline-5-carboxylate reductase -
  K0H61_RS05095 (K0H61_05095) - 1135668..1136378 (-) 711 WP_220051658.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H61_RS05100 (K0H61_05100) pilT 1136414..1137451 (+) 1038 WP_011621982.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H61_RS05105 (K0H61_05105) pilU 1137461..1138573 (+) 1113 WP_220051659.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H61_RS05110 (K0H61_05110) - 1138707..1139273 (+) 567 WP_220051660.1 glutathione peroxidase -
  K0H61_RS05115 (K0H61_05115) hemH 1139311..1140324 (+) 1014 WP_220051661.1 ferrochelatase -
  K0H61_RS05120 (K0H61_05120) ruvX 1140397..1140822 (-) 426 WP_220051662.1 Holliday junction resolvase RuvX -
  K0H61_RS05125 (K0H61_05125) - 1140849..1141412 (-) 564 WP_220051663.1 YqgE/AlgH family protein -
  K0H61_RS05130 (K0H61_05130) yciH 1141491..1141820 (-) 330 WP_220051664.1 stress response translation initiation inhibitor YciH -
  K0H61_RS05135 (K0H61_05135) - 1141971..1142282 (-) 312 WP_220051665.1 hypothetical protein -
  K0H61_RS05140 (K0H61_05140) - 1142461..1143021 (+) 561 WP_220051666.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41153.23 Da        Isoelectric Point: 6.2359

>NTDB_id=592647 K0H61_RS05105 WP_220051659.1 1137461..1138573(+) (pilU) [Shewanella acanthi strain FJAT-51860]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKTEFHTTRECNFAFAAKELGR
FRVSAFWQRESSGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEVLINTPRVSSLIAKNELHLLKETMAKSNEQGMQTFDQA
LLNLYLENEISYADALHHADSPNDLRLMIKLQNKEPSSSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592647 K0H61_RS05105 WP_220051659.1 1137461..1138573(+) (pilU) [Shewanella acanthi strain FJAT-51860]
ATGGATGTCCGTCCGTTTTTAAAAGTGATGGTAGAGCGTAAAGCCTCGGATTTGTTTATTACCGCAGGTTTTCCGCCGAG
TGCAAAAATCGATGGTGAGCTTCGCCCTTTGGCCGAAAGCGCGTTTACGCCTGCCCAATCCCTCGATTTTGTTGAGTCTT
TGATGACTGAGGCGCAAAAGACCGAGTTCCATACGACACGTGAATGTAACTTTGCATTTGCGGCTAAGGAGTTAGGTCGT
TTCCGTGTCAGTGCGTTTTGGCAACGTGAATCTTCTGGCTGCGTGATGCGTCGAATTGAGACGAAAATCCCAGAAGTAGA
CGACTTAAAACTGCCGCCCATCCTAAAAGATCTGGTGATGAGTAAGCGTGGTCTTATCATCATGGTGGGGGGGACTGGTA
CCGGTAAATCGACGTCACTCGCGGCCTTGGTGGGTTATCGTAACGCCCATGCCCGTGGTCATATTCTGACTATCGAAGAT
CCTGTGGAGTTCGTCCACGACCATCGTAAGAGCATTATCACCCAGCGTGAAGTGGGTATTGATACCGATTCCTTTGATGC
AGCCCTTAAGAGTTCATTGCGTCAGGCGCCTGACGTGATCCTTATCGGTGAGATTCGTACTCAAGAAACCATGGAGTTTG
CTCTGTCGTTTGCCGAAACCGGTCACCTTTGTATGGCGACACTTCACGCCAACAACGCTAACCAAGCCTTAGACCGGATT
ATGCACTTAGTGCCAGAGAGTAAGCATCAGCAGTTGCTGTTCGACCTGTCATTAAACCTGCGTGGTATTGTTGCTCAACA
ACTCGTGCCTAAGGCCGATGGTACAGGGCGTCGCGCTGCAATCGAAGTGCTGATTAATACTCCGCGGGTGTCGAGTTTAA
TTGCTAAAAATGAGCTGCATTTGCTCAAAGAAACAATGGCTAAGTCGAATGAGCAAGGCATGCAGACCTTCGACCAAGCG
TTGCTTAATCTGTATCTTGAAAATGAGATCAGTTACGCCGATGCACTGCACCATGCTGACTCGCCTAACGACCTACGTTT
GATGATTAAGCTGCAAAATAAAGAGCCGAGCAGTTCAAGCTTTATGGAAGGGGTTACCCTAGATATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Acinetobacter baylyi ADP1

61.364

95.135

0.584
  pilU Vibrio cholerae strain A1552

57.027

100

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.26

86.216

0.373
  pilT Vibrio cholerae strain A1552

43.26

86.216

0.373
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365