Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0H60_RS06400 Genome accession   NZ_CP080412
Coordinates   1438775..1439812 (+) Length   345 a.a.
NCBI ID   WP_011716282.1    Uniprot ID   A0A2M7HST3
Organism   Shewanella mangrovisoli strain FJAT-51754     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1433775..1444812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H60_RS06370 (K0H60_06370) rdgB 1434788..1435405 (-) 618 WP_220057617.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0H60_RS06375 (K0H60_06375) - 1435566..1436000 (-) 435 WP_011621977.1 DUF4426 domain-containing protein -
  K0H60_RS06380 (K0H60_06380) yggU 1436069..1436359 (-) 291 WP_011621978.1 DUF167 family protein YggU -
  K0H60_RS06385 (K0H60_06385) - 1436359..1436907 (-) 549 WP_011716279.1 YggT family protein -
  K0H60_RS06390 (K0H60_06390) proC 1436983..1437801 (-) 819 WP_220057618.1 pyrroline-5-carboxylate reductase -
  K0H60_RS06395 (K0H60_06395) - 1438023..1438739 (-) 717 WP_220057619.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H60_RS06400 (K0H60_06400) pilT 1438775..1439812 (+) 1038 WP_011716282.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H60_RS06405 (K0H60_06405) pilU 1439822..1440934 (+) 1113 WP_011621983.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H60_RS06410 (K0H60_06410) - 1441110..1441643 (+) 534 WP_086904528.1 glutathione peroxidase -
  K0H60_RS06415 (K0H60_06415) hemH 1441713..1442696 (+) 984 WP_220057620.1 ferrochelatase -
  K0H60_RS06420 (K0H60_06420) ruvX 1442758..1443183 (-) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  K0H60_RS06425 (K0H60_06425) - 1443214..1443777 (-) 564 WP_011621987.1 YqgE/AlgH family protein -
  K0H60_RS06430 (K0H60_06430) yciH 1443846..1444175 (-) 330 WP_023266511.1 stress response translation initiation inhibitor YciH -
  K0H60_RS06435 (K0H60_06435) - 1444325..1444639 (-) 315 WP_011716287.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.02 Da        Isoelectric Point: 6.9097

>NTDB_id=592623 K0H60_RS06400 WP_011716282.1 1438775..1439812(+) (pilT) [Shewanella mangrovisoli strain FJAT-51754]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=592623 K0H60_RS06400 WP_011716282.1 1438775..1439812(+) (pilT) [Shewanella mangrovisoli strain FJAT-51754]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAGCATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCCCGCGGCGCGGCGGCGGTATTTCGTACCATTCCAAGTGAAATTTTGTCGCTCGA
GCAGTTAGGGGCGCCTGAGATTTTTAAAAAAATTTCCAGCTTTCCCCGCGGCTTAGTGCTTGTTACTGGGCCTACTGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAGAACCGCCACGACCATATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAGAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGCCATACCCACAGCTTTAACGC
GGCGCTACGTAGCGCACTGCGTGAAGACCCTGACGTTATTCTCGTCGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACGGCGGCTGAAACGGGTCACTTAGTTTTTGGTACCTTGCATACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTTTTCCCTGCTGGTGAAAAGGACATGGTGCGTACCATGTTATCTGAATCATTACAGGCGGTTATTTCGCAAAC
CCTGATTAAGAAAATCGGTGGTGGCCGTGTGGCTGCCCACGAAATCATGATGGGTACGCCCGCTATTCGTAACCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCAGCCATTCAAACGGGGATGGCCCATGGCATGCAAACGCTCGAACAGTGTCTG
CAAAACTTAGTCAACCGTGGCCTCATCACCCGTGAGGATGCCATGTCGAAGAGTTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2M7HST3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.592

99.42

0.791
  pilT Acinetobacter baumannii strain A118

79.592

99.42

0.791
  pilT Acinetobacter nosocomialis M2

79.592

99.42

0.791
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

80.547

95.362

0.768
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.369

97.391

0.403
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388