Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KY496_RS23225 Genome accession   NZ_CP080379
Coordinates   5254862..5256046 (+) Length   394 a.a.
NCBI ID   WP_219862638.1    Uniprot ID   -
Organism   Massilia sp. NP310     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5249862..5261046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KY496_RS23210 (KY496_23210) - 5251322..5251894 (+) 573 WP_219862636.1 PEP-CTERM sorting domain-containing protein -
  KY496_RS23215 (KY496_23215) - 5252118..5253788 (+) 1671 WP_219865161.1 mechanosensitive ion channel family protein -
  KY496_RS23220 (KY496_23220) - 5253766..5254707 (-) 942 WP_184235541.1 LysR substrate-binding domain-containing protein -
  KY496_RS23225 (KY496_23225) pilU 5254862..5256046 (+) 1185 WP_219862638.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KY496_RS23230 (KY496_23230) - 5256068..5256541 (+) 474 WP_005668097.1 adenylyltransferase/cytidyltransferase family protein -
  KY496_RS23235 (KY496_23235) - 5256550..5256915 (-) 366 WP_219862639.1 hypothetical protein -
  KY496_RS23240 (KY496_23240) - 5256944..5258338 (-) 1395 WP_229520347.1 FAD-binding protein -
  KY496_RS23245 (KY496_23245) - 5258489..5259667 (+) 1179 WP_219862641.1 GDSL-type esterase/lipase family protein -
  KY496_RS23250 (KY496_23250) - 5259731..5260228 (+) 498 WP_219862643.1 cupin domain-containing protein -
  KY496_RS23255 (KY496_23255) - 5260242..5260799 (+) 558 WP_219862645.1 dihydrofolate reductase family protein -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43735.07 Da        Isoelectric Point: 6.3725

>NTDB_id=592437 KY496_RS23225 WP_219862638.1 5254862..5256046(+) (pilU) [Massilia sp. NP310]
MSSTFGPAEAQAYIHKLLTVMYHQGGSDLFISADFPPSIKHQGAMKALSQQRLTGEVTRALALSIMNERQRAEFEAEMEC
NFAISLPGVCRFRVNVYVQQQSVGMVVRTIASEIPNFEKLDLPEVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
NSAGHIITVEDPVEYVHKNKNCLVTHREVGVDTLSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTPTIGEMILKGNFQNIKEIMHKS
RELGMCTFDQALFELYNKGLIAYDEAIRNADSANGLRLQIKLSGERREPGAAAARPAELSMAIEEEPDDEPPKP

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=592437 KY496_RS23225 WP_219862638.1 5254862..5256046(+) (pilU) [Massilia sp. NP310]
ATGTCCTCCACGTTCGGCCCGGCGGAAGCCCAGGCCTATATACACAAGCTCCTGACGGTGATGTACCACCAGGGCGGCTC
CGACCTCTTCATCTCGGCTGACTTTCCTCCAAGCATTAAACACCAGGGGGCGATGAAAGCGCTCAGCCAGCAGCGCCTGA
CCGGCGAAGTCACGCGCGCGCTGGCGCTGTCGATCATGAACGAGCGCCAGCGCGCCGAGTTCGAGGCCGAGATGGAGTGC
AATTTTGCGATTTCCCTGCCGGGCGTGTGCCGCTTCCGCGTCAACGTCTATGTGCAGCAGCAGAGCGTGGGCATGGTGGT
GCGCACGATCGCGTCCGAGATTCCCAACTTCGAAAAGCTCGACCTGCCCGAGGTGCTCAAGGACGTGGTGATGACCAAGC
GCGGCCTGGTGCTGGTGGTGGGCGGCACCGGTTCCGGCAAGTCGACCACGCTGGCGGCAATGATCGACTACCGCAACAGC
AACTCGGCCGGCCACATCATCACGGTGGAAGACCCGGTGGAATACGTCCACAAGAACAAGAATTGCCTGGTGACGCACCG
CGAAGTGGGCGTGGACACCCTGTCGTGGCATCACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACACCGAGACGATGGAACATGCGATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGGCACCCTGCAC
GCCAATAACGCCAACCAGACGATGGACCGGATCATCAACTTCTTCCCGGAGGAGCGGCGCAATCAGCTGTTGATGGATTT
GTCGTCGAACCTGCGCGCCATCGTCTCGCAGCGCCTGGTGCGCACCGAGGATGGCAAGGGCCGCAAGGCGGCCATCGAGA
TCCTGCTGAACACGCCGACCATCGGCGAGATGATCCTCAAAGGGAACTTCCAGAACATCAAGGAGATCATGCACAAGTCG
CGCGAACTGGGCATGTGCACCTTCGACCAGGCCTTGTTCGAGTTGTACAACAAGGGCCTGATCGCCTACGACGAGGCGAT
CCGCAATGCCGACTCGGCCAACGGCCTGCGCCTGCAGATCAAGCTGTCGGGTGAGCGACGAGAGCCGGGCGCCGCTGCCG
CGCGGCCGGCCGAGCTGTCGATGGCGATCGAGGAAGAACCCGACGACGAACCGCCCAAACCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.714

88.832

0.53
  pilU Acinetobacter baylyi ADP1

52.198

92.386

0.482
  pilU Vibrio cholerae strain A1552

50.287

88.325

0.444
  pilT Pseudomonas aeruginosa PAK

44.745

84.518

0.378
  pilT Legionella pneumophila strain ERS1305867

44.985

83.503

0.376
  pilT Legionella pneumophila strain Lp02

44.985

83.503

0.376
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

85.533

0.376
  pilT Pseudomonas stutzeri DSM 10701

43.917

85.533

0.376
  pilT Acinetobacter baylyi ADP1

43.598

83.249

0.363