Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   LPB400_RS03185 Genome accession   NZ_CP079818
Coordinates   626547..627788 (-) Length   413 a.a.
NCBI ID   WP_219089370.1    Uniprot ID   -
Organism   Neisseria perflava strain LPB0400     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 621547..632788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPB400_RS03155 (LPB400_03160) ispH 622238..623206 (-) 969 WP_219089364.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  LPB400_RS03160 (LPB400_03165) lspA 623220..623726 (-) 507 WP_219089366.1 signal peptidase II -
  LPB400_RS03165 (LPB400_03170) glyQ 623878..624777 (-) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  LPB400_RS03170 (LPB400_03175) yacG 624859..625041 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  LPB400_RS03175 (LPB400_03180) coaE 625038..625658 (-) 621 WP_219089368.1 dephospho-CoA kinase -
  LPB400_RS03180 (LPB400_03185) pilD 625658..626545 (-) 888 WP_225905497.1 A24 family peptidase Machinery gene
  LPB400_RS03185 (LPB400_03190) pilG 626547..627788 (-) 1242 WP_219089370.1 type II secretion system F family protein Machinery gene
  LPB400_RS03190 (LPB400_03195) pilF 627868..629547 (-) 1680 WP_070824176.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LPB400_RS03195 (LPB400_03200) - 629940..631001 (-) 1062 WP_219089372.1 nucleotidyltransferase domain-containing protein -
  LPB400_RS03200 (LPB400_03205) - 631016..631774 (-) 759 WP_219089374.1 nucleotidyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45934.06 Da        Isoelectric Point: 9.9459

>NTDB_id=589632 LPB400_RS03185 WP_219089370.1 626547..627788(-) (pilG) [Neisseria perflava strain LPB0400]
MAQAEQKRSLFGSRSKGKRFTFEGKNIETERLVRGEVVAKDEEEARKKLQRRGIRPLRISKVKTARKRRITQEDITVFTR
QLATMMKAGLPLMQAFEIVARGHSNPSMTEMLMQVRSDVEQGSALGKSFSKYPKYFDRFYCNLVSAGESGGVLESLLDKL
AVYKEKTQAIKKKVKTALTYPIAIIVVAIALIFIMMMFVLPAFKEVYANMGAELPGLTQLVMSLSDLFVDYGWIMIILLI
VSAFGLYKLHEKSPTFQKRIDALILRLPVFGTIVRKATIARWARTTSTLFAAGVPLVEVLDSVAGASGNILYEEATQDIR
AKVTQGLSLTSSMQSTDMFPNMVIQMAAIGEESGSLDDMLNKAAEFYEDEVDNSVSRLSHLMEPIIMVVLGSLIGILLIA
MYLPLFNLGNVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=589632 LPB400_RS03185 WP_219089370.1 626547..627788(-) (pilG) [Neisseria perflava strain LPB0400]
ATGGCTCAAGCAGAGCAAAAAAGAAGTCTGTTCGGCTCAAGAAGCAAAGGCAAGCGTTTTACATTTGAAGGAAAAAACAT
CGAGACCGAACGTCTTGTTCGTGGTGAAGTAGTTGCCAAAGACGAAGAAGAAGCACGCAAAAAACTCCAACGCAGAGGCA
TTCGCCCTTTGCGTATCAGCAAAGTCAAAACCGCACGCAAACGCCGTATTACCCAAGAAGACATTACTGTGTTCACCCGC
CAACTGGCAACAATGATGAAGGCAGGCTTACCGCTGATGCAGGCATTTGAAATCGTTGCACGCGGCCACTCCAATCCGAG
CATGACCGAAATGCTGATGCAGGTTCGCTCCGATGTCGAACAAGGTAGCGCATTGGGTAAATCGTTCTCTAAATATCCAA
AATATTTCGACCGTTTCTATTGCAATCTGGTCAGCGCAGGCGAGTCCGGCGGCGTACTGGAAAGCCTATTGGACAAACTG
GCTGTCTATAAAGAAAAAACCCAAGCCATTAAGAAAAAGGTTAAAACCGCGCTGACCTACCCGATTGCCATTATCGTGGT
GGCCATCGCGCTTATCTTCATCATGATGATGTTTGTACTGCCAGCCTTTAAAGAAGTTTATGCCAATATGGGTGCAGAGC
TGCCAGGCCTTACCCAACTCGTCATGAGCTTGTCTGATTTATTCGTCGATTACGGCTGGATTATGATCATCCTTTTGATT
GTTTCCGCCTTCGGCCTGTACAAACTCCATGAAAAATCGCCTACCTTCCAAAAACGAATCGATGCTCTGATTTTGAGACT
GCCTGTTTTTGGTACCATTGTCCGAAAAGCGACCATTGCCCGTTGGGCGCGTACGACTTCCACCCTCTTCGCAGCCGGCG
TTCCTTTGGTGGAAGTATTAGATTCAGTTGCAGGCGCATCCGGCAATATTCTCTATGAAGAAGCTACTCAAGACATCCGT
GCCAAGGTAACACAAGGTCTGTCGCTGACATCCAGTATGCAAAGCACGGATATGTTCCCCAATATGGTAATCCAAATGGC
GGCCATCGGCGAAGAGTCAGGCTCTTTGGATGATATGCTGAACAAAGCAGCCGAATTCTACGAAGATGAAGTAGACAACT
CCGTCTCCCGATTGTCTCACTTGATGGAGCCTATTATCATGGTGGTATTGGGTTCGCTCATCGGTATTTTGCTGATTGCC
ATGTATCTGCCGTTGTTTAACTTGGGTAATGTCGTAGGTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

72.03

97.821

0.705
  pilG Neisseria meningitidis 44/76-A

71.04

97.821

0.695
  pilC Legionella pneumophila strain ERS1305867

46.348

96.126

0.446
  pilC Pseudomonas stutzeri DSM 10701

46

96.852

0.446
  pilC Acinetobacter baumannii D1279779

46.231

96.368

0.446
  pilC Acinetobacter baylyi ADP1

45.729

96.368

0.441
  pilC Vibrio cholerae strain A1552

38.5

96.852

0.373
  pilC Thermus thermophilus HB27

38.363

94.673

0.363