Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LPB400_RS03190 Genome accession   NZ_CP079818
Coordinates   627868..629547 (-) Length   559 a.a.
NCBI ID   WP_070824176.1    Uniprot ID   A0A9X9HYH5
Organism   Neisseria perflava strain LPB0400     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 622868..634547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPB400_RS03160 (LPB400_03165) lspA 623220..623726 (-) 507 WP_219089366.1 signal peptidase II -
  LPB400_RS03165 (LPB400_03170) glyQ 623878..624777 (-) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  LPB400_RS03170 (LPB400_03175) yacG 624859..625041 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  LPB400_RS03175 (LPB400_03180) coaE 625038..625658 (-) 621 WP_219089368.1 dephospho-CoA kinase -
  LPB400_RS03180 (LPB400_03185) pilD 625658..626545 (-) 888 WP_225905497.1 A24 family peptidase Machinery gene
  LPB400_RS03185 (LPB400_03190) pilG 626547..627788 (-) 1242 WP_219089370.1 type II secretion system F family protein Machinery gene
  LPB400_RS03190 (LPB400_03195) pilF 627868..629547 (-) 1680 WP_070824176.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LPB400_RS03195 (LPB400_03200) - 629940..631001 (-) 1062 WP_219089372.1 nucleotidyltransferase domain-containing protein -
  LPB400_RS03200 (LPB400_03205) - 631016..631774 (-) 759 WP_219089374.1 nucleotidyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62255.51 Da        Isoelectric Point: 6.0846

>NTDB_id=589633 LPB400_RS03190 WP_070824176.1 627868..629547(-) (pilF) [Neisseria perflava strain LPB0400]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLRYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=589633 LPB400_RS03190 WP_070824176.1 627868..629547(-) (pilF) [Neisseria perflava strain LPB0400]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAAACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGGGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTGGTCGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCGCTACTACCCACGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCTACCCAAATCCA
AAACTTTCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTCGTGGTTCCAGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGTCAACGTTCGACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACACAGCAATCGCAATCG
CTTTATATCGACAATGAAGAAGCTGAAGACGGCCCTATCCCCCGCTTCATCCACAAAACTTTATCAGATGCCCTGAACGC
TGGCGCATCAGACATCCACTTTGAATTCTACGAACAAATGGCGCGCGTTCGTTTCCGTGTGGACGGACAATTGCGTGAAG
TCGTACAACCTCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
GACATTGTTTGGCGAAAAAGTGGTGATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAACTCGGCTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATCAACCGCCCTTACGGCATGGTATTGGTTACCGGTCCGACAGGTTCG
GGCAAAACCGTTTCGCTCTATACTTGCCTGAGTATTTTGAACACGGAAGATGTGAACATTTCGACGGCAGAAGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGCCTGACGTTTGCTGCCGCACTCCGATCCT
TCTTACGTCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCTACCTTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
TGCGCCTTTCAACATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTTGAACGCCCTCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAACTC
TACCGCCCGGTTGGTTGTGACAGCTGCCGCGGTAAAGGATTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
TGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGAGCCGGTCTCATGAAAGTCATGCAGGGCCTGACTTCGTTGGAAGAAGTGACTGCCCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.639

100

0.776
  pilB Acinetobacter baylyi ADP1

51.056

100

0.519
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.602

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.547

96.064

0.37