Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BRPE64_RS11520 Genome accession   NC_021287
Coordinates   2496722..2497912 (+) Length   396 a.a.
NCBI ID   WP_016346301.1    Uniprot ID   R4WIG6
Organism   Caballeronia insecticola     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491722..2502912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRPE64_RS11490 (BRPE64_ACDS23940) cgtA 2491939..2493033 (-) 1095 WP_016346296.1 Obg family GTPase CgtA -
  BRPE64_RS11495 (BRPE64_ACDS23950) rpmA 2493208..2493471 (-) 264 WP_044041586.1 50S ribosomal protein L27 -
  BRPE64_RS11500 (BRPE64_ACDS23960) rplU 2493507..2493818 (-) 312 WP_016346298.1 50S ribosomal protein L21 -
  BRPE64_RS11505 (BRPE64_ACDS23970) - 2494092..2495084 (+) 993 WP_044041588.1 polyprenyl synthetase family protein -
  BRPE64_RS11515 (BRPE64_ACDS23980) - 2495358..2496647 (+) 1290 WP_016346300.1 HlyC/CorC family transporter -
  BRPE64_RS11520 (BRPE64_ACDS23990) pilB 2496722..2497912 (+) 1191 WP_016346301.1 GspE/PulE family protein Machinery gene
  BRPE64_RS11525 (BRPE64_ACDS24000) - 2497909..2499135 (+) 1227 WP_016346302.1 type II secretion system F family protein -
  BRPE64_RS11530 (BRPE64_ACDS24010) - 2499167..2500087 (+) 921 WP_016346303.1 A24 family peptidase -
  BRPE64_RS11535 (BRPE64_ACDS24020) coaE 2500104..2500709 (+) 606 WP_016346304.1 dephospho-CoA kinase -
  BRPE64_RS11540 (BRPE64_ACDS24030) zapD 2500880..2501635 (+) 756 WP_016346305.1 cell division protein ZapD -
  BRPE64_RS11545 (BRPE64_ACDS24040) - 2501706..2501897 (+) 192 WP_016346306.1 DNA gyrase inhibitor YacG -
  BRPE64_RS11550 (BRPE64_ACDS24050) - 2501943..2502383 (-) 441 WP_016346307.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 42837.12 Da        Isoelectric Point: 7.3105

>NTDB_id=58864 BRPE64_RS11520 WP_016346301.1 2496722..2497912(+) (pilB) [Caballeronia insecticola]
MISPSRDAFDSFTRDPSSDHEAPAVSLLTDILREAAERGASDIHVEPAEHDWRIRMRVDGVLHASRRPPPHLRDAFVTRI
KVLARMDIAERRVPQDGRLRLKLPGGRAGDYRVNSLPTLFGEKLVLRRLDTLPPDLSLAALGLDDAQGRVVESAIRAPHG
LVLVTGPTGSGKTLSLYCFLQMLNAEARNVCSVEDPVEIQLAGINQVSVREKAGLTFAVALRAFMRQDPDIIMVGEIRDA
ETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVAPYNLASALRLVTAQRLVRKLCEHCRAPSALPFGDGFHPFEPR
GCPACHGIGYRGRVGVHQLMPVSDELRELIVTRAATHAIARAAQAQGMPALRDAALERVRAGVTSVAEAAEATESE

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=58864 BRPE64_RS11520 WP_016346301.1 2496722..2497912(+) (pilB) [Caballeronia insecticola]
ATGATTTCTCCCTCCCGCGACGCCTTCGACTCTTTCACGCGCGACCCTTCGTCCGATCACGAAGCGCCCGCCGTCTCGCT
CCTCACCGACATCCTGCGCGAAGCCGCCGAGCGCGGCGCGTCGGATATTCACGTCGAACCCGCCGAGCACGACTGGCGCA
TCCGCATGCGCGTCGATGGCGTGCTGCATGCGTCGAGGCGTCCGCCGCCGCATTTGCGCGATGCCTTCGTCACGCGCATC
AAGGTGCTGGCGCGCATGGACATTGCCGAGCGGCGGGTACCGCAGGACGGCAGGCTGCGTCTTAAGCTGCCGGGCGGACG
AGCGGGCGACTATCGCGTCAACTCGCTGCCGACGCTGTTCGGCGAGAAGCTCGTCCTGCGACGGCTCGACACGCTCCCGC
CCGATCTGTCGCTTGCCGCGCTCGGCCTCGACGACGCTCAGGGCCGCGTCGTCGAATCGGCGATACGCGCGCCGCATGGT
CTCGTTCTCGTGACGGGACCGACCGGAAGCGGCAAAACGCTGTCGCTCTACTGCTTCTTGCAGATGTTGAATGCCGAAGC
GCGCAACGTGTGCTCGGTGGAAGACCCCGTCGAGATCCAGCTCGCGGGCATCAATCAGGTGAGCGTGCGCGAGAAAGCCG
GCCTGACTTTCGCGGTGGCGCTGCGCGCGTTCATGCGCCAGGATCCGGACATCATCATGGTCGGAGAAATTCGCGATGCC
GAAACCGCGGACGTCGCCGTGAAGGCCGCGCAAACCGGCCATCTCGTGCTCTCGACGCTCCACACGAACGACGCCCCCGC
CGCGATTGCGCGACTCATCGATATTGGCGTGGCGCCGTATAACCTCGCGTCGGCGTTGCGTCTCGTCACGGCACAGCGGC
TTGTGCGCAAGCTGTGCGAGCACTGCCGCGCGCCATCGGCGCTGCCGTTCGGCGACGGCTTTCATCCGTTCGAGCCGCGC
GGCTGTCCGGCGTGTCATGGCATTGGCTATCGGGGGCGCGTCGGCGTGCATCAACTGATGCCGGTGTCGGACGAGTTGCG
CGAACTGATCGTCACACGCGCCGCCACACATGCCATTGCGCGCGCGGCGCAGGCGCAAGGCATGCCCGCGCTGCGCGATG
CGGCCTTGGAGCGCGTGCGGGCGGGCGTGACGAGCGTCGCGGAAGCGGCCGAAGCCACGGAGAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-297)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R4WIG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

50.251

100

0.505
  pilB Acinetobacter baumannii D1279779

50.255

98.99

0.497
  pilB Acinetobacter baylyi ADP1

49.223

97.475

0.48
  pilF Neisseria gonorrhoeae MS11

47.848

99.747

0.477
  pilB Vibrio campbellii strain DS40M4

48.421

95.96

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

48.158

95.96

0.462
  pilB Legionella pneumophila strain ERS1305867

46.667

98.485

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

44.472

100

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.878

99.495

0.417
  pilF Thermus thermophilus HB27

42.338

97.222

0.412
  pilB Haemophilus influenzae Rd KW20

42.667

94.697

0.404
  pilB Haemophilus influenzae 86-028NP

42.4

94.697

0.402
  pilB Glaesserella parasuis strain SC1401

41.689

92.677

0.386
  ctsE Campylobacter jejuni subsp. jejuni 81-176

38.874

94.192

0.366

Multiple sequence alignment