Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KEC60_RS01945 Genome accession   NZ_CP079237
Coordinates   395500..395892 (-) Length   130 a.a.
NCBI ID   WP_284649322.1    Uniprot ID   -
Organism   Photobacterium damselae strain Pdd1608     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390500..400892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEC60_RS01925 (KEC60_01925) coaE 390855..391448 (-) 594 WP_284649318.1 dephospho-CoA kinase -
  KEC60_RS01930 (KEC60_01930) pilD 391450..392328 (-) 879 WP_284649319.1 A24 family peptidase Machinery gene
  KEC60_RS01935 (KEC60_01935) pilC 392510..393736 (-) 1227 WP_284649320.1 type II secretion system F family protein Machinery gene
  KEC60_RS01940 (KEC60_01940) pilB 393802..395493 (-) 1692 WP_284649321.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KEC60_RS01945 (KEC60_01945) pilA 395500..395892 (-) 393 WP_284649322.1 pilin Machinery gene
  KEC60_RS01950 (KEC60_01950) ampD 396279..396857 (+) 579 WP_284649323.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KEC60_RS01955 (KEC60_01955) pdhR 397217..397990 (+) 774 WP_284649324.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  KEC60_RS01960 (KEC60_01960) aceE 398071..400731 (+) 2661 WP_068945542.1 pyruvate dehydrogenase (acetyl-transferring), homodimeric type -

Sequence

Protein


Download         Length: 130 a.a.        Molecular weight: 13729.92 Da        Isoelectric Point: 5.0937

>NTDB_id=588353 KEC60_RS01945 WP_284649322.1 395500..395892(-) (pilA) [Photobacterium damselae strain Pdd1608]
MKGQKGFTLIELMIVVAIIGVLSAIAIPAYKDYVKKSQMASAMATMKSLITPAELEIQENGSISGGISTLGMSAGANTLG
IITANSDNLVFKFVGGAMNDDTLTFTRTDTDGWSCDRGNKTEMPKIEGCK

Nucleotide


Download         Length: 393 bp        

>NTDB_id=588353 KEC60_RS01945 WP_284649322.1 395500..395892(-) (pilA) [Photobacterium damselae strain Pdd1608]
ATGAAAGGACAAAAGGGTTTTACCTTAATTGAATTGATGATCGTTGTAGCGATTATTGGTGTGTTATCAGCAATTGCGAT
TCCAGCTTACAAAGACTATGTTAAAAAAAGTCAAATGGCATCCGCAATGGCGACTATGAAATCATTAATTACTCCTGCAG
AACTCGAAATACAGGAAAATGGCTCAATAAGTGGTGGAATTTCAACACTAGGAATGTCAGCAGGGGCAAATACACTGGGA
ATCATTACAGCTAATAGTGACAACCTAGTCTTTAAATTTGTTGGGGGAGCGATGAATGATGACACGCTAACATTTACAAG
GACAGATACCGATGGTTGGTCATGTGATCGAGGTAACAAAACTGAAATGCCAAAGATTGAAGGTTGTAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

42.667

100

0.492
  pilA Vibrio parahaemolyticus RIMD 2210633

52.5

92.308

0.485
  pilA Vibrio cholerae strain A1552

37.5

100

0.415
  pilA Vibrio cholerae C6706

37.5

100

0.415
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.5

100

0.415
  pilA Acinetobacter baumannii strain A118

36.62

100

0.4
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.516

95.385

0.377
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.095

96.923

0.369