Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L38_RS00200 Genome accession   NZ_CP077394
Coordinates   37895..39004 (-) Length   369 a.a.
NCBI ID   WP_010636179.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1408     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 32895..44004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L38_RS00180 (I6L38_00180) brnQ 33352..34659 (-) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -
  I6L38_RS00185 (I6L38_00185) - 34851..35561 (-) 711 WP_043171430.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  I6L38_RS00190 (I6L38_00190) srmB 35690..36913 (+) 1224 WP_010636177.1 ATP-dependent RNA helicase SrmB -
  I6L38_RS00195 (I6L38_00195) yaaA 37090..37863 (-) 774 WP_043171429.1 peroxide stress protein YaaA -
  I6L38_RS00200 (I6L38_00200) pilU 37895..39004 (-) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  I6L38_RS00205 (I6L38_00205) pilT 39033..40067 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  I6L38_RS00210 (I6L38_00210) - 40107..40808 (+) 702 WP_010636180.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L38_RS00215 (I6L38_00215) proC 40869..41693 (+) 825 WP_010636181.1 pyrroline-5-carboxylate reductase -
  I6L38_RS00220 (I6L38_00220) - 41734..42285 (+) 552 WP_005305000.1 YggT family protein -
  I6L38_RS00225 (I6L38_00225) yggU 42285..42584 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  I6L38_RS00230 (I6L38_00230) - 42603..43022 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  I6L38_RS00235 (I6L38_00235) - 43086..43397 (-) 312 WP_005304992.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41081.22 Da        Isoelectric Point: 6.5672

>NTDB_id=581104 I6L38_RS00200 WP_010636179.1 37895..39004(-) (pilU) [Aeromonas sp. FDAARGOS 1408]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=581104 I6L38_RS00200 WP_010636179.1 37895..39004(-) (pilU) [Aeromonas sp. FDAARGOS 1408]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAATGGCCACCTGCAGTCGCTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCACTGGCGCTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTACCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATAGCCCTGGCCAAGCGCGGACTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCATCGTTCGATGT
GGCACTGAAGAGCTCGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCATGCCAACAACGCCAACCAGGCTCTGGATCGCATC
TTGCACCTGGTGCCCCAGGAGAAACACCGCCAGTTCCTGTTCGACCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAACA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAGCAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363