Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L46_RS19745 Genome accession   NZ_CP077301
Coordinates   4329022..4330131 (+) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1416     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4324022..4335131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L46_RS19715 (I6L46_19715) - 4324923..4325342 (-) 420 WP_005340339.1 DUF4426 domain-containing protein -
  I6L46_RS19720 (I6L46_19720) yggU 4325386..4325688 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  I6L46_RS19725 (I6L46_19725) - 4325688..4326239 (-) 552 WP_005341416.1 YggT family protein -
  I6L46_RS19730 (I6L46_19730) proC 4326251..4327075 (-) 825 WP_216976863.1 pyrroline-5-carboxylate reductase -
  I6L46_RS19735 (I6L46_19735) - 4327218..4327919 (-) 702 WP_100654584.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L46_RS19740 (I6L46_19740) pilT 4327959..4328993 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  I6L46_RS19745 (I6L46_19745) pilU 4329022..4330131 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  I6L46_RS19750 (I6L46_19750) yaaA 4330179..4330952 (+) 774 WP_064338818.1 peroxide stress protein YaaA -
  I6L46_RS19755 (I6L46_19755) - 4331147..4331572 (+) 426 WP_005340360.1 hypothetical protein -
  I6L46_RS19760 (I6L46_19760) - 4331932..4332423 (-) 492 WP_216976865.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  I6L46_RS19765 (I6L46_19765) arfB 4332465..4332881 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  I6L46_RS19770 (I6L46_19770) glnK 4333083..4333421 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  I6L46_RS19775 (I6L46_19775) - 4333680..4334687 (+) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=580668 I6L46_RS19745 WP_005340356.1 4329022..4330131(+) (pilU) [Aeromonas sp. FDAARGOS 1416]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=580668 I6L46_RS19745 WP_005340356.1 4329022..4330131(+) (pilU) [Aeromonas sp. FDAARGOS 1416]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGTGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCGCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGGCGATGATTGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTAAAAAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACTGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAACGGCTTGGCACCGGCACGCTGGACAATGTGACACTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36