Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L34_RS13815 Genome accession   NZ_CP077237
Coordinates   2968764..2969873 (+) Length   369 a.a.
NCBI ID   WP_058057996.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1404     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2963764..2974873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L34_RS13785 (I6L34_13785) - 2964665..2965084 (-) 420 WP_113740088.1 DUF4426 domain-containing protein -
  I6L34_RS13790 (I6L34_13790) yggU 2965128..2965430 (-) 303 WP_021230942.1 DUF167 family protein YggU -
  I6L34_RS13795 (I6L34_13795) - 2965430..2965981 (-) 552 WP_005341416.1 YggT family protein -
  I6L34_RS13800 (I6L34_13800) proC 2965993..2966817 (-) 825 WP_216954853.1 pyrroline-5-carboxylate reductase -
  I6L34_RS13805 (I6L34_13805) - 2966960..2967661 (-) 702 WP_216954854.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L34_RS13810 (I6L34_13810) pilT 2967701..2968735 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  I6L34_RS13815 (I6L34_13815) pilU 2968764..2969873 (+) 1110 WP_058057996.1 type IVa pilus ATPase TapU Machinery gene
  I6L34_RS13820 (I6L34_13820) yaaA 2969921..2970694 (+) 774 WP_058057969.1 peroxide stress protein YaaA -
  I6L34_RS13825 (I6L34_13825) - 2970889..2971314 (+) 426 WP_005340360.1 hypothetical protein -
  I6L34_RS13830 (I6L34_13830) - 2971674..2972165 (-) 492 WP_058057970.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  I6L34_RS13835 (I6L34_13835) arfB 2972198..2972614 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  I6L34_RS13840 (I6L34_13840) glnK 2972816..2973154 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  I6L34_RS13845 (I6L34_13845) - 2973413..2974420 (+) 1008 WP_152985455.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41053.08 Da        Isoelectric Point: 6.4327

>NTDB_id=580146 I6L34_RS13815 WP_058057996.1 2968764..2969873(+) (pilU) [Aeromonas sp. FDAARGOS 1404]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALANADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=580146 I6L34_RS13815 WP_058057996.1 2968764..2969873(+) (pilU) [Aeromonas sp. FDAARGOS 1404]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTTGTGTCGCTGGGGGGGGAGGCGCTCGACAAGAAGGGGGCACTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACTCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGATGT
GGCGCTAAAAAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACGGGCCATCTTTGTCTCGCTACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATT
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GTTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCCGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACATTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCAACGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain Lp02

41.667

87.805

0.366
  pilT Legionella pneumophila strain ERS1305867

41.667

87.805

0.366
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36