Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KSS96_RS03005 Genome accession   NZ_CP077079
Coordinates   671935..673263 (+) Length   442 a.a.
NCBI ID   WP_017526523.1    Uniprot ID   -
Organism   Pseudomonas asgharzadehiana strain SWRI132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 666935..678263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSS96_RS02985 (KSS96_02985) - 667013..669475 (-) 2463 WP_116078970.1 penicillin-binding protein 1A -
  KSS96_RS02990 (KSS96_02990) pilM 669693..670346 (+) 654 WP_017526525.1 type IV pilus biogenesis protein PilM -
  KSS96_RS02995 (KSS96_02995) - 670343..670897 (+) 555 WP_217855714.1 PilN domain-containing protein -
  KSS96_RS03000 (KSS96_03000) - 670894..671865 (+) 972 WP_217855716.1 pilus assembly protein PilP -
  KSS96_RS03005 (KSS96_03005) pilQ 671935..673263 (+) 1329 WP_017526523.1 type IV pilus secretin PilQ Machinery gene
  KSS96_RS03010 (KSS96_03010) aroK 673268..673786 (+) 519 WP_003171222.1 shikimate kinase AroK -
  KSS96_RS03015 (KSS96_03015) aroB 673902..675002 (+) 1101 WP_017526522.1 3-dehydroquinate synthase -
  KSS96_RS03020 (KSS96_03020) - 675013..676569 (+) 1557 WP_017526521.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 48199.31 Da        Isoelectric Point: 4.9501

>NTDB_id=578775 KSS96_RS03005 WP_017526523.1 671935..673263(+) (pilQ) [Pseudomonas asgharzadehiana strain SWRI132]
MVVAVPNRVDLIQLPPPVSAAVLDAYTGDKLNLNFQNIELRAVLQQIADVAGLNLVASDDVQGSITLRLKGVPWDQALDL
VLQTKGLDKRVKAGVLLVAPAEELAARELLTLESRKQMADLAPLRRELLQVNYAKAADLAKLFQSVNGLEGTTDERGSVA
VDDRTNNIIAYQTHERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGHWSAGGINKPLAEGAEPP
ENPPSSPFVDMGSLTAASGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATS
VSFKEASLSLEVTPQITPDDHVIMVVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVP
FLGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=578775 KSS96_RS03005 WP_017526523.1 671935..673263(+) (pilQ) [Pseudomonas asgharzadehiana strain SWRI132]
ATGGTTGTTGCTGTGCCCAATCGTGTGGATCTGATCCAACTACCACCTCCGGTGAGCGCCGCGGTATTGGATGCCTATAC
CGGTGACAAACTCAATCTCAACTTCCAGAACATCGAGTTGCGCGCCGTCTTGCAGCAAATTGCCGATGTGGCGGGCCTCA
ACCTGGTGGCCAGCGACGACGTGCAAGGCTCGATTACGCTGCGACTCAAGGGCGTGCCTTGGGATCAGGCCCTGGACCTG
GTGCTGCAAACCAAGGGGTTGGATAAACGGGTAAAAGCCGGCGTATTGCTGGTGGCGCCCGCTGAAGAGTTGGCTGCGCG
CGAGTTGCTCACCTTGGAGTCACGCAAGCAAATGGCTGACCTGGCGCCACTGCGTCGTGAGTTGCTGCAGGTCAATTACG
CCAAGGCGGCGGACCTGGCCAAGTTGTTTCAGTCGGTGAACGGGCTGGAAGGCACGACCGATGAGCGCGGCTCGGTGGCA
GTGGATGATCGCACGAACAATATCATCGCTTACCAGACCCACGAGCGGCTTGAAGAGCTGCGGCGGATCGTGGCGCAGTT
GGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGGATTGTCGAGGCGAATGTCGACTACGACAAAAGCCTGGGTGTGC
GCTGGGGTGGGCGGCTCAATCGTGGTCATTGGAGTGCCGGCGGTATCAATAAACCCTTGGCCGAGGGGGCGGAGCCGCCG
GAAAACCCACCCAGCTCGCCGTTTGTCGACATGGGGTCGCTCACCGCTGCTTCAGGCCTGGGTATTGCCTACATCACCGA
CAATTTGCTGCTCGACCTGGAACTGACGGCCATGGAGAAAACCGGCAACGGCGAGATTGTCTCGCAGCCCAAGGTGGTCA
CCTCCGACAAGGAGACCGCACGTATCCTGAAGGGCACCGAAATTCCCTATCAGGAATCCACCTCCCAGGGTGCAACCTCC
GTGTCATTCAAGGAGGCCTCGCTGTCCCTGGAGGTTACGCCGCAAATCACCCCCGACGACCATGTGATCATGGTGGTCAA
AGTCACCAAGGATGAGCCCGACTACCTGAACAAACTCAATGACGTACCGCCCATCAAGAAAAACGAGGTCAACGCCAAGG
TGCTGGTCAAGGATGGCGAGACCATCGTTATTGGCGGAGTTTTCTCCAATACCCAGAGCAAAGTGGTAGATAAAGTGCCA
TTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATT
CCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(54-100)

Bacterial type II and III secretion system protein (Secretin)

(277-433)

Bacterial type II/III secretion system short domain (Secretin_N)

(128-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.709

98.869

0.699
  comQ Acinetobacter baylyi ADP1

42.35

100

0.432
  pilQ Acinetobacter baumannii D1279779

41.037

100

0.43
  pilQ Acinetobacter baumannii strain A118

41.037

100

0.43
  pilQ Vibrio campbellii strain DS40M4

43.972

95.701

0.421
  pilQ Legionella pneumophila strain Lp02

44.391

94.796

0.421
  pilQ Legionella pneumophila strain ERS1305867

44.391

94.796

0.421
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.258

94.57

0.419
  pilQ Vibrio cholerae strain A1552

44.258

94.57

0.419
  pilQ Neisseria meningitidis M1080

37.209

97.285

0.362