Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KSS95_RS04635 Genome accession   NZ_CP077073
Coordinates   1005289..1006557 (+) Length   422 a.a.
NCBI ID   WP_217852058.1    Uniprot ID   -
Organism   Pseudomonas muyukensis strain COW39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1000289..1011557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSS95_RS04615 (KSS95_04615) - 1000360..1002813 (-) 2454 WP_217852051.1 penicillin-binding protein 1A -
  KSS95_RS04620 (KSS95_04620) pilM 1002983..1003822 (+) 840 WP_217852053.1 type IV pilus biogenesis protein PilM -
  KSS95_RS04625 (KSS95_04625) - 1003819..1004358 (+) 540 WP_217852055.1 PilN domain-containing protein -
  KSS95_RS04630 (KSS95_04630) - 1004355..1005365 (+) 1011 WP_217852056.1 pilus assembly protein PilP -
  KSS95_RS04635 (KSS95_04635) pilQ 1005289..1006557 (+) 1269 WP_217852058.1 type IV pilus secretin PilQ Machinery gene
  KSS95_RS04640 (KSS95_04640) aroK 1006562..1007080 (+) 519 WP_217852060.1 shikimate kinase AroK -
  KSS95_RS04645 (KSS95_04645) aroB 1007179..1008276 (+) 1098 WP_217852062.1 3-dehydroquinate synthase -
  KSS95_RS04650 (KSS95_04650) - 1008292..1009902 (+) 1611 WP_217852064.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45926.03 Da        Isoelectric Point: 5.9178

>NTDB_id=578637 KSS95_RS04635 WP_217852058.1 1005289..1006557(+) (pilQ) [Pseudomonas muyukensis strain COW39]
MKQGRWLGGLLFMLMGMRGALATEGEPLSLNFQDVEVRAVLQIMADYVGVNLVASDTVQGRITLRLEQVPWQQALDLVLR
SRGLGHRQEGNVLLVAPLAELAERSTGVRQEQALQAQLAPLQRALIPVFHAKAAELAELLMASLADDGILAGRGAVSVDA
RTNTLVVAQPARQLAELRKLVAQLDVPVRQVMIEARIVEASVDYEKTLGVRWGGPLYGQDSRLGSELFVDLGVERATSGL
GLGLLRSDILLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSISFREASLSLEVTPQITPDG
KVVMAVRVTKDEPDFINALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSHNKVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=578637 KSS95_RS04635 WP_217852058.1 1005289..1006557(+) (pilQ) [Pseudomonas muyukensis strain COW39]
ATGAAACAGGGGCGATGGCTGGGCGGGTTGCTGTTCATGCTGATGGGGATGCGCGGCGCCTTGGCGACAGAAGGTGAGCC
GCTGTCGCTGAACTTCCAGGACGTCGAAGTTCGCGCGGTCCTGCAGATCATGGCGGATTATGTGGGTGTCAACCTGGTGG
CCAGCGACACCGTGCAAGGGCGGATCACCTTGCGTCTGGAGCAGGTGCCTTGGCAGCAGGCCCTTGACCTGGTGCTGCGC
AGTCGGGGCCTTGGTCATCGTCAGGAGGGTAACGTGCTGCTGGTCGCGCCCTTGGCGGAACTGGCCGAGCGGTCTACGGG
CGTTCGCCAGGAGCAGGCCCTGCAAGCGCAGTTGGCGCCGCTGCAACGTGCATTGATCCCGGTGTTCCATGCCAAGGCGG
CGGAGCTGGCCGAGCTGCTGATGGCCAGCCTGGCCGACGACGGCATCCTCGCCGGGCGTGGCGCCGTCAGTGTGGATGCG
CGTACCAATACCTTGGTGGTCGCCCAGCCTGCGCGGCAGTTGGCGGAGTTGCGCAAGCTGGTGGCGCAGCTGGACGTGCC
GGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAGCGTCGATTATGAAAAGACCCTCGGCGTCCGTTGGGGCG
GGCCGCTGTATGGCCAGGACAGCCGCCTGGGCAGCGAGCTGTTCGTCGACCTTGGTGTCGAGCGCGCCACCTCGGGCCTG
GGGCTCGGCCTGCTGCGCAGCGACATTCTGCTCGACCTGGAGCTCAGTGCCATGGAAAAGAGCGGCAACGGCGAGATCAT
CTCCCAGCCCAAGGTGGTCACCGCCGACAAGGAAACCGCGCGGATCCTCAAGGGTACCGAGGTGCCCTACCAGGAAACCA
CCAGAAGCGGCGCCACCTCCATTTCCTTTCGCGAGGCCTCGCTGTCGCTGGAGGTGACCCCGCAGATCACCCCCGACGGC
AAGGTGGTGATGGCGGTACGGGTGACCAAGGACGAGCCGGACTTCATCAATGCCCTGAACAACGTGCCGCCGATTCGCAA
GAACGAGGTCAATGCCAAGGTGCGCGTGGCCGATGGCGAAACCATTGTGATTGGTGGCGTGTACTCGACGTCGCATAACA
AAGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGACGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.447

100

0.607
  comQ Acinetobacter baylyi ADP1

44.018

100

0.462
  pilQ Acinetobacter baumannii D1279779

42.232

100

0.457
  pilQ Acinetobacter baumannii strain A118

42.232

100

0.457
  pilQ Legionella pneumophila strain Lp02

43.269

98.578

0.427
  pilQ Legionella pneumophila strain ERS1305867

43.269

98.578

0.427
  pilQ Vibrio campbellii strain DS40M4

40.465

100

0.412
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.232

100

0.412
  pilQ Vibrio cholerae strain A1552

41.232

100

0.412
  pilQ Neisseria gonorrhoeae strain FA1090

37.019

98.578

0.365