Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   TOL_RS15625 Genome accession   NC_020888
Coordinates   3403381..3405480 (-) Length   699 a.a.
NCBI ID   WP_015488342.1    Uniprot ID   M5DVQ8
Organism   Thalassolituus oleivorans MIL-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3398381..3410480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL_RS15610 (TOL_3235) - 3400286..3401734 (-) 1449 WP_015488339.1 SPOR domain-containing protein -
  TOL_RS15615 (TOL_3236) aroB 3401752..3402843 (-) 1092 WP_015488340.1 3-dehydroquinate synthase -
  TOL_RS15620 (TOL_3237) aroK 3402858..3403376 (-) 519 WP_197019469.1 shikimate kinase AroK -
  TOL_RS15625 (TOL_3238) pilQ 3403381..3405480 (-) 2100 WP_015488342.1 type IV pilus secretin PilQ Machinery gene
  TOL_RS15630 (TOL_3239) - 3405483..3406022 (-) 540 WP_015488343.1 pilus assembly protein PilP -
  TOL_RS15635 (TOL_3240) - 3406024..3406662 (-) 639 WP_015488344.1 type 4a pilus biogenesis protein PilO -
  TOL_RS15640 (TOL_3241) - 3406664..3407218 (-) 555 WP_015488345.1 PilN domain-containing protein -
  TOL_RS15645 (TOL_3242) pilM 3407218..3408315 (-) 1098 WP_015488346.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75406.82 Da        Isoelectric Point: 4.7659

>NTDB_id=57642 TOL_RS15625 WP_015488342.1 3403381..3405480(-) (pilQ) [Thalassolituus oleivorans MIL-1]
MKIIKGLRQAVFAVPAMLLLMSADALAVTLKDIKFSTMAGDITEIALEFDGAPPEVSGYTIEKPARIAIDLPGVQSALQT
KRHEIGNGNARSATIVATKDRARLVVNLTQLAAYTTTANGNTLLVRIGERSEEKVFAGNTVTSASGASVSPASDMKVVNV
DFRRGELGEGQIQIMLNDARASANIRREGSKIIADLSGVRLPDVLSRRLDVTDFATPVKYVDARREDGVTRIVIEPTSDN
FEYLAYQTDKLLSINVSEPPKDQKEAQKKQFPFTGEKLSLNFQNIEVRAVLQLIADFTGLNLVASDSVQGAITLRLQNVP
WDQALDLVLKTKGLGQRQMGSVLLIAPAAEIAAREKVELEAVRQVEELAPLVTEYMQLKYAKASVLATLLTSEQGLLSER
GTAVVDERTNTLLMKDTSANLEKVREAIIMLDVPVRQVLIEARIVVANTSVGKELGVKWGGASFKDNGSNWTTAGGSQQT
LTEGSQILFDRATTGSSTQNIDLTNANVVDFGATNAAASTFAIGYQTADFLLDLELSAIETEGRAEIVSQPRVITADGQT
ASIESGTEIPYQQASSSGATNVAFKSAVLRLEVTPQITPDDRIIMDLLINQDSVGEQTSAGPSINTNAVETQVLVENGET
VVLGGIFRSEDVTTIRKTPFFGDLPLIGALFRYKSKTDTKSELLVFITPRLVKDTLSTR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=57642 TOL_RS15625 WP_015488342.1 3403381..3405480(-) (pilQ) [Thalassolituus oleivorans MIL-1]
ATGAAAATAATTAAGGGCTTACGTCAGGCTGTATTCGCGGTGCCGGCAATGTTATTGCTGATGTCTGCTGATGCCTTGGC
TGTCACACTGAAAGACATAAAATTTTCAACCATGGCGGGTGATATTACCGAGATCGCGTTGGAGTTTGATGGGGCGCCGC
CAGAAGTTAGTGGTTACACCATTGAGAAGCCTGCACGTATCGCAATTGACTTACCAGGTGTGCAGAGTGCTCTACAAACC
AAGCGTCATGAAATTGGTAACGGTAATGCGCGCAGTGCGACCATTGTGGCGACTAAAGATCGTGCGCGTCTCGTCGTGAA
TTTAACTCAGTTGGCGGCTTACACAACAACAGCCAACGGTAATACCTTACTTGTTCGAATCGGAGAGCGTTCGGAAGAGA
AAGTATTCGCTGGAAACACAGTTACTAGTGCTTCAGGCGCTTCAGTTAGTCCTGCTAGCGATATGAAAGTCGTTAACGTT
GATTTCCGTCGTGGAGAGCTAGGTGAGGGTCAAATTCAGATCATGCTGAACGATGCGCGAGCGTCTGCCAACATACGTCG
TGAAGGTTCAAAAATTATTGCAGATTTAAGTGGCGTGCGTTTACCTGACGTATTAAGCCGTCGACTTGATGTCACTGATT
TTGCCACGCCCGTTAAATATGTTGATGCGCGTCGTGAAGATGGCGTAACGCGTATTGTGATTGAGCCGACTTCGGATAAC
TTTGAGTATTTGGCCTATCAAACCGATAAATTGTTATCGATCAACGTATCTGAGCCACCAAAAGATCAAAAAGAAGCACA
GAAGAAGCAATTTCCATTTACCGGTGAAAAGCTATCTCTTAACTTCCAAAATATCGAAGTTCGCGCTGTGTTGCAGTTGA
TTGCGGACTTCACTGGTTTGAACTTAGTTGCCTCAGATTCAGTGCAGGGGGCGATTACGCTGCGTTTGCAGAATGTGCCT
TGGGATCAAGCATTGGATTTAGTGCTAAAAACCAAAGGTTTAGGGCAGCGTCAAATGGGCTCTGTGCTCTTGATTGCACC
AGCGGCTGAAATTGCTGCGCGTGAAAAAGTGGAGCTCGAAGCGGTTCGTCAAGTTGAAGAGCTGGCACCGTTAGTCACGG
AATACATGCAGCTCAAGTATGCCAAAGCATCTGTACTGGCTACATTGTTGACGTCGGAGCAAGGTTTGTTGTCAGAGCGC
GGTACTGCGGTAGTTGATGAGCGTACTAATACATTGCTAATGAAAGACACTTCTGCAAACTTAGAAAAAGTACGCGAAGC
TATCATTATGCTGGACGTTCCTGTTCGCCAAGTGCTGATTGAAGCTCGCATTGTGGTAGCAAACACCAGTGTTGGTAAAG
AACTCGGCGTGAAATGGGGTGGGGCTTCGTTTAAAGACAATGGCAGCAATTGGACTACTGCTGGTGGCAGTCAGCAGACC
TTAACGGAAGGCTCGCAGATCTTGTTTGACCGCGCGACCACTGGCAGTTCTACACAAAATATCGATTTGACCAACGCCAA
CGTAGTGGATTTCGGTGCTACAAACGCAGCAGCGTCAACGTTCGCGATTGGATATCAAACAGCAGACTTCTTGTTGGATC
TAGAGTTGTCGGCGATTGAAACCGAAGGTCGTGCTGAAATTGTATCTCAGCCGCGTGTGATTACGGCGGATGGTCAGACT
GCCTCTATTGAGTCAGGTACAGAAATTCCATACCAGCAAGCTAGCTCAAGTGGCGCGACTAACGTGGCCTTTAAGTCAGC
TGTTTTACGCTTGGAAGTGACTCCGCAAATTACTCCTGATGATCGAATTATCATGGATTTGCTCATTAATCAGGACTCTG
TTGGCGAGCAAACCTCAGCTGGTCCTAGTATCAATACCAATGCGGTTGAGACTCAAGTGTTAGTTGAGAATGGCGAAACA
GTAGTACTTGGTGGTATCTTCCGTTCTGAAGATGTGACCACCATCCGTAAAACACCGTTTTTTGGCGATTTACCTCTGAT
TGGTGCGTTGTTCCGCTATAAGAGTAAGACGGATACTAAGAGTGAGCTGCTTGTGTTCATTACCCCTCGTTTGGTGAAGG
ATACGCTCAGCACTCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-437)

Bacterial type II and III secretion system protein (Secretin)

(538-693)

AMIN domain (AMIN)

(41-127)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5DVQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.515

100

0.491
  pilQ Acinetobacter baumannii strain A118

41.918

100

0.438
  pilQ Acinetobacter baumannii D1279779

41.918

100

0.438
  comQ Acinetobacter baylyi ADP1

40.111

100

0.412
  pilQ Legionella pneumophila strain Lp02

39.97

94.134

0.376
  pilQ Legionella pneumophila strain ERS1305867

40.031

93.276

0.373
  pilQ Neisseria gonorrhoeae strain FA1090

34.631

100

0.369

Multiple sequence alignment