Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TOL_RS15645 Genome accession   NC_020888
Coordinates   3407218..3408315 (-) Length   365 a.a.
NCBI ID   WP_015488346.1    Uniprot ID   -
Organism   Thalassolituus oleivorans MIL-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3402218..3413315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL_RS15620 (TOL_3237) aroK 3402858..3403376 (-) 519 WP_197019469.1 shikimate kinase AroK -
  TOL_RS15625 (TOL_3238) pilQ 3403381..3405480 (-) 2100 WP_015488342.1 type IV pilus secretin PilQ Machinery gene
  TOL_RS15630 (TOL_3239) - 3405483..3406022 (-) 540 WP_015488343.1 pilus assembly protein PilP -
  TOL_RS15635 (TOL_3240) - 3406024..3406662 (-) 639 WP_015488344.1 type 4a pilus biogenesis protein PilO -
  TOL_RS15640 (TOL_3241) - 3406664..3407218 (-) 555 WP_015488345.1 PilN domain-containing protein -
  TOL_RS15645 (TOL_3242) pilM 3407218..3408315 (-) 1098 WP_015488346.1 pilus assembly protein PilM Machinery gene
  TOL_RS15650 (TOL_3243) - 3408448..3411042 (+) 2595 WP_015488347.1 penicillin-binding protein 1A -
  TOL_RS15655 (TOL_3244) - 3411105..3412508 (-) 1404 WP_015488348.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 39515.55 Da        Isoelectric Point: 4.2678

>NTDB_id=57643 TOL_RS15645 WP_015488346.1 3407218..3408315(-) (pilM) [Thalassolituus oleivorans MIL-1]
MQAREFDGDFVVLASLFNKKNKALLGVDISSTSVKVLELSRQGDRYQVEAYATEPLPLNAVVEQSISNDEAVGEAIRKAI
ARSRTSAKAAAIAVAGSAVITKTVHMSATLKDDEMDFQIRAEADQYIPYPLEEVALDWEIQGPSEGLADMVDVLLAACRS
ETVDRRKDAVEYAGISVERVDVEAFCTERAFALLGAQVVGEDVETVAVLDIGATMTTLSVLHEGKSIYTREQLFGGKQLT
EDIKRRYGLSDEEAQRAKIEGGVPDDYESEVLDPFRQSVVQQVSRSLQFFYSSSQFNDVDCIVLAGGTSAIAGLADRVQE
TLGIPTVVANPFVSMTLSQKVNANLLNSDAPSLMIAAGLALRSFD

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=57643 TOL_RS15645 WP_015488346.1 3407218..3408315(-) (pilM) [Thalassolituus oleivorans MIL-1]
ATGCAAGCTCGCGAATTTGATGGGGATTTTGTCGTGCTGGCTAGCCTGTTTAACAAGAAAAATAAGGCGCTTCTGGGCGT
GGACATAAGCTCAACGTCAGTCAAAGTGCTCGAGCTCAGCCGTCAAGGCGATCGCTATCAGGTGGAAGCCTATGCGACAG
AGCCTCTCCCGCTCAATGCGGTTGTAGAGCAGAGTATTAGCAATGACGAAGCGGTAGGTGAAGCAATTCGTAAAGCAATT
GCTCGTTCGCGCACGAGTGCAAAAGCAGCAGCCATTGCGGTTGCTGGCTCAGCGGTTATTACTAAAACCGTTCATATGAG
TGCGACTCTTAAAGACGATGAGATGGATTTTCAGATTCGCGCAGAAGCGGATCAGTATATTCCATACCCTCTCGAAGAGG
TCGCGCTTGATTGGGAAATTCAAGGCCCATCAGAAGGATTAGCCGATATGGTTGATGTCCTTTTGGCTGCTTGTCGCTCT
GAAACCGTTGATCGTCGTAAAGACGCAGTCGAATATGCCGGTATTTCGGTAGAGCGCGTCGACGTTGAAGCATTTTGCAC
AGAGCGTGCCTTTGCTCTCTTGGGGGCGCAGGTTGTTGGTGAAGATGTGGAGACCGTCGCAGTTCTTGATATTGGCGCGA
CCATGACCACGCTTAGTGTTCTTCATGAAGGGAAATCAATTTACACTCGCGAACAGTTGTTCGGGGGGAAGCAATTGACG
GAAGACATCAAAAGACGTTACGGCTTGTCGGATGAAGAAGCGCAGCGTGCAAAAATAGAAGGCGGTGTTCCTGATGATTA
TGAATCTGAAGTCTTAGATCCATTTCGCCAGTCGGTTGTGCAGCAAGTGAGTCGTTCATTGCAATTTTTCTATTCATCAA
GTCAATTTAATGATGTCGACTGTATTGTTTTAGCAGGTGGTACGTCAGCGATTGCTGGATTGGCAGATAGAGTTCAAGAA
ACTCTGGGTATCCCTACCGTGGTGGCAAATCCTTTCGTTAGCATGACGCTGTCGCAAAAGGTAAATGCGAATTTGTTAAA
TAGTGATGCACCTTCTCTAATGATTGCGGCAGGATTGGCATTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(26-364)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.714

95.89

0.515
  comM Acinetobacter nosocomialis M2

53.714

95.89

0.515
  comM Acinetobacter baylyi ADP1

50.286

95.89

0.482
  pilM Legionella pneumophila strain ERS1305867

43.099

97.26

0.419

Multiple sequence alignment