Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KN217_RS04240 Genome accession   NZ_CP076348
Coordinates   875527..876744 (+) Length   405 a.a.
NCBI ID   WP_213662719.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain DN36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 870527..881744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KN217_RS04225 (KN217_04210) cysN 870945..872843 (+) 1899 WP_301619906.1 sulfate adenylyltransferase subunit CysN -
  KN217_RS04230 (KN217_04215) - 873061..873501 (-) 441 WP_301619907.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KN217_RS04235 (KN217_04220) pilB 873822..875525 (+) 1704 WP_213662718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KN217_RS04240 (KN217_04225) pilC 875527..876744 (+) 1218 WP_213662719.1 type II secretion system F family protein Machinery gene
  KN217_RS04245 (KN217_04230) pilD 876748..877617 (+) 870 WP_301619908.1 A24 family peptidase Machinery gene
  KN217_RS04250 (KN217_04235) coaE 877722..878324 (+) 603 WP_301619909.1 dephospho-CoA kinase -
  KN217_RS04255 (KN217_04240) yacG 878324..878521 (+) 198 WP_301619910.1 DNA gyrase inhibitor YacG -
  KN217_RS04260 (KN217_04245) - 878568..879251 (-) 684 WP_155551575.1 energy-coupling factor ABC transporter permease -
  KN217_RS04265 (KN217_04250) rplM 879666..880094 (+) 429 WP_009867981.1 50S ribosomal protein L13 -
  KN217_RS04270 (KN217_04255) rpsI 880109..880501 (+) 393 WP_009867980.1 30S ribosomal protein S9 -
  KN217_RS04275 (KN217_04260) petA 880751..881344 (+) 594 WP_009867979.1 ubiquinol-cytochrome c reductase iron-sulfur subunit -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44144.86 Da        Isoelectric Point: 9.9609

>NTDB_id=574808 KN217_RS04240 WP_213662719.1 875527..876744(+) (pilC) [Stutzerimonas stutzeri strain DN36]
MAQKAIKTSVFTWEGTDRKGAKIKGELSGVSPALVKAQLRKQGVNPQKVRKKSVSLFGAGKKIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDELKQEVAAGNSFAASLRKKPQYFDDLYCNLVDSGEQSGSLETLLDRVATYKEKT
EALKAKIKKAMNYPIAVVVVAIIVSAILLIKVVPQFQEVFANFGAELPAFTLMVIGLSEALQEWWFVVLASLIAAAFAFK
QAHRRSEKFRNWFDRVLLKVPVVGDILYKSAVARFTRTLSTTFAAGVPLVDALDSVAGATGNVVFRNATMKVKNDVSTGM
QLNFSMRTTGTFPSMAVQMTAIGEESGSLDEMLGKVATFYEDEVDNMVDGLTSLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=574808 KN217_RS04240 WP_213662719.1 875527..876744(+) (pilC) [Stutzerimonas stutzeri strain DN36]
ATGGCGCAGAAAGCGATAAAAACCAGTGTCTTCACCTGGGAAGGGACCGACCGCAAGGGCGCAAAGATCAAGGGAGAGCT
GAGTGGGGTCAGCCCCGCACTGGTCAAGGCGCAGCTACGCAAGCAGGGTGTCAACCCGCAAAAGGTGCGTAAGAAGTCGG
TGTCATTGTTCGGTGCCGGCAAGAAGATCAAGCCTCTGGATATCGCGTTATTCACGCGGCAGATGGCGACCATGATGAAA
GCGGGGGTTCCGCTGCTGCAGTCGTTCGACATCATCGGCGAGGGTTTCGACAATCCCAACATGCGTAAGCTGGTGGACGA
ACTGAAGCAGGAGGTTGCCGCAGGCAATAGTTTTGCGGCTTCGCTGCGCAAGAAACCGCAATATTTTGACGATCTGTATT
GCAACCTGGTGGATTCCGGCGAGCAGTCCGGTTCGCTGGAGACGCTGCTCGATCGCGTGGCGACCTACAAGGAAAAGACC
GAGGCCCTGAAAGCCAAGATCAAGAAGGCGATGAACTATCCGATCGCGGTGGTGGTGGTGGCGATCATCGTTTCGGCGAT
CCTGCTGATCAAGGTGGTGCCGCAGTTTCAGGAGGTGTTTGCCAACTTCGGCGCTGAGCTGCCTGCGTTCACACTGATGG
TGATTGGGCTTTCGGAGGCTTTGCAGGAGTGGTGGTTCGTCGTTCTGGCTAGCCTGATTGCCGCAGCTTTTGCCTTCAAG
CAAGCGCACCGGCGCTCCGAGAAGTTTCGCAACTGGTTCGATCGCGTTCTGCTGAAGGTGCCGGTCGTGGGTGACATTCT
TTATAAATCCGCCGTTGCCCGCTTCACACGTACGCTATCAACCACCTTCGCCGCTGGCGTACCGCTCGTCGATGCTCTGG
ATTCGGTAGCGGGCGCAACCGGGAACGTGGTGTTTCGCAATGCGACGATGAAGGTCAAGAATGACGTCTCCACCGGTATG
CAGCTGAACTTCTCGATGCGCACGACCGGCACGTTCCCTTCGATGGCAGTGCAGATGACCGCGATCGGCGAAGAGTCCGG
CTCGCTCGACGAGATGTTGGGCAAGGTGGCCACCTTCTATGAGGATGAGGTGGACAACATGGTCGACGGCCTTACCAGTT
TGATGGAGCCAATGATCATGGCGGTTCTGGGCGTGCTGGTGGGCGGGTTGATCATTGCGATGTATCTGCCGATCTTCCAG
TTGGGGGCAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

91.358

100

0.914
  pilC Acinetobacter baumannii D1279779

60.494

100

0.605
  pilC Acinetobacter baylyi ADP1

60.099

100

0.602
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

44.776

99.259

0.444
  pilG Neisseria meningitidis 44/76-A

44.776

99.259

0.444
  pilC Vibrio cholerae strain A1552

41.919

97.778

0.41
  pilC Vibrio campbellii strain DS40M4

40.657

97.778

0.398