Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KN217_RS04235 Genome accession   NZ_CP076348
Coordinates   873822..875525 (+) Length   567 a.a.
NCBI ID   WP_213662718.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain DN36     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 868822..880525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KN217_RS04215 (KN217_04200) - 869091..869849 (+) 759 WP_301619904.1 Nif3-like dinuclear metal center hexameric protein -
  KN217_RS04220 (KN217_04205) cysD 870015..870932 (+) 918 WP_301619905.1 sulfate adenylyltransferase subunit CysD -
  KN217_RS04225 (KN217_04210) cysN 870945..872843 (+) 1899 WP_301619906.1 sulfate adenylyltransferase subunit CysN -
  KN217_RS04230 (KN217_04215) - 873061..873501 (-) 441 WP_301619907.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KN217_RS04235 (KN217_04220) pilB 873822..875525 (+) 1704 WP_213662718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KN217_RS04240 (KN217_04225) pilC 875527..876744 (+) 1218 WP_213662719.1 type II secretion system F family protein Machinery gene
  KN217_RS04245 (KN217_04230) pilD 876748..877617 (+) 870 WP_301619908.1 A24 family peptidase Machinery gene
  KN217_RS04250 (KN217_04235) coaE 877722..878324 (+) 603 WP_301619909.1 dephospho-CoA kinase -
  KN217_RS04255 (KN217_04240) yacG 878324..878521 (+) 198 WP_301619910.1 DNA gyrase inhibitor YacG -
  KN217_RS04260 (KN217_04245) - 878568..879251 (-) 684 WP_155551575.1 energy-coupling factor ABC transporter permease -
  KN217_RS04265 (KN217_04250) rplM 879666..880094 (+) 429 WP_009867981.1 50S ribosomal protein L13 -
  KN217_RS04270 (KN217_04255) rpsI 880109..880501 (+) 393 WP_009867980.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61929.99 Da        Isoelectric Point: 5.4602

>NTDB_id=574807 KN217_RS04235 WP_213662718.1 873822..875525(+) (pilB) [Stutzerimonas stutzeri strain DN36]
MSDNISLSGLARQMVLAGVLDEKTAQQAQVQALRSKASLVSYVVQNKLAKGRAVAELASEQFGVALLDLSAVDKETMPKE
LVSEKLARQHRLLPLARRGNKLYVAVSDPANHQAVTDVQFSTGLHIEAILVEDDKLGQALDKFYESDATGLEDLGDVDLD
GLDVETVTDDAKGDEGGGDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEAQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTIDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPGCKKEIDVPRDTLLDEGFP
ADRLGTFKIYAPVGCDNCKGGYKGRVGIYEVVKITPALQRIIMEDGNSIDIATQMRKDGFNDLRTSALAKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=574807 KN217_RS04235 WP_213662718.1 873822..875525(+) (pilB) [Stutzerimonas stutzeri strain DN36]
ATGAGCGACAATATTTCCCTTTCGGGCCTGGCCCGGCAGATGGTGCTGGCCGGCGTGCTGGATGAAAAGACCGCCCAGCA
GGCCCAGGTACAGGCGTTGCGCAGCAAGGCCTCGCTGGTCAGCTATGTGGTGCAGAACAAGCTGGCGAAAGGGCGTGCGG
TGGCCGAACTGGCGTCCGAGCAATTCGGTGTCGCGCTGCTGGATCTGTCGGCGGTGGACAAGGAAACCATGCCGAAGGAG
CTGGTAAGCGAGAAACTGGCGCGCCAGCATCGGCTGCTGCCGCTGGCACGGCGTGGCAACAAGCTCTATGTTGCGGTGTC
GGACCCAGCCAACCATCAGGCCGTTACCGATGTCCAGTTCAGCACCGGGCTGCATATCGAGGCCATCCTGGTCGAGGATG
ACAAGCTCGGCCAGGCGCTGGACAAGTTCTATGAAAGCGATGCGACCGGGCTGGAAGATCTGGGCGACGTCGACCTGGAT
GGCCTCGATGTCGAAACCGTCACCGATGACGCCAAGGGCGATGAGGGGGGCGGCGACGCCGACGATGCACCGGTGGTGCG
CTTCGTCAACAAGATGCTGCTCGATGCGATTCGCGGTGGTTCGTCCGACCTGCATTTCGAACCCTATGAGAAGGCCTACC
GTGTTCGGTTGCGTACCGATGGCATCCTGCACGAGGTGGCGCGACCGCCGATCCAACTCGCGCCGCGCATCTCGGCACGC
CTCAAAGTAATGGCTGGGCTGGATATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGCATCAAGATGAAGATATCCAAAAC
CAAGGCCATCGACTTTCGTGTCAACACGCTGCCGACGCTGTGGGGGGAGAAGATCGTAATGCGGATTCTCGATCCATCCA
GTGCGCAGATGGGCATCGATGCGTTGGGTTATGAGGAGGCGCAGAAGGAGCTGTACATGAATGCCTTGCGCCAGCCGCAG
GGCATGATCCTGGTGACCGGGCCGACCGGCTCCGGCAAGACGGTTTCGCTGTATACCGGCCTGAACATCCTCAACACTAT
AGATGTGAATATCTCGACGGCCGAAGACCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAACCCTCGTC
AGGGCATGGACTTCGCCCAGGCGCTGCGTGCGTTCCTGCGCCAGGACCCGGACATCATCATGGTTGGCGAGATTCGCGAC
CTGGAAACCGCTGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCGACGCTGCACACCAACAGCGCTGC
TGAGACGCTGACGCGCCTGCGCAACATGGGCGTGCCGGCGTTCAACATTGCTACGTCGGTGAACCTGATCATCGCCCAGC
GCTTGGCGCGCAAGCTCTGTCCGGGCTGCAAGAAAGAAATCGACGTGCCGCGCGATACTCTGCTGGACGAGGGCTTCCCT
GCCGATCGACTCGGTACGTTCAAAATATACGCCCCGGTGGGTTGCGATAACTGCAAGGGGGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCTGCATTGCAGCGCATTATCATGGAAGACGGCAATTCAATCGATATAGCCACCC
AGATGCGTAAAGACGGTTTCAATGATCTGCGAACTTCGGCACTTGCGAAGGCCATGCAGGGCGTTACCAGTCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.752

99.471

0.594
  pilB Acinetobacter baylyi ADP1

59.503

99.295

0.591
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

49.824

100

0.499
  pilB Vibrio cholerae strain A1552

51.287

95.944

0.492
  pilF Neisseria gonorrhoeae MS11

49.027

99.647

0.489
  pilB Vibrio campbellii strain DS40M4

48.161

100

0.485
  pilB/pilB1 Synechocystis sp. PCC 6803

36.688

100

0.399