Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KN217_RS01445 Genome accession   NZ_CP076348
Coordinates   310626..311771 (+) Length   381 a.a.
NCBI ID   WP_008568506.1    Uniprot ID   A0A8E2QBM6
Organism   Stutzerimonas stutzeri strain DN36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 305626..316771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KN217_RS01425 (KN217_01415) - 307345..307935 (-) 591 WP_008568510.1 YggT family protein -
  KN217_RS01430 (KN217_01420) proC 307945..308769 (-) 825 WP_301619617.1 pyrroline-5-carboxylate reductase -
  KN217_RS01435 (KN217_01425) - 308780..309469 (-) 690 WP_213662449.1 YggS family pyridoxal phosphate-dependent enzyme -
  KN217_RS01440 (KN217_01430) pilT 309536..310570 (+) 1035 WP_008568507.1 type IV pilus twitching motility protein PilT Machinery gene
  KN217_RS01445 (KN217_01435) pilU 310626..311771 (+) 1146 WP_008568506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KN217_RS01450 (KN217_01440) - 311888..312283 (+) 396 WP_301619618.1 TM2 domain-containing protein -
  KN217_RS01455 (KN217_01445) - 312367..313638 (-) 1272 WP_301619619.1 dihydroorotase -
  KN217_RS01460 (KN217_01450) - 313638..314654 (-) 1017 WP_008568501.1 aspartate carbamoyltransferase catalytic subunit -
  KN217_RS01465 (KN217_01455) pyrR 314664..315176 (-) 513 WP_008568500.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  KN217_RS01470 (KN217_01460) ruvX 315199..315627 (-) 429 WP_301619620.1 Holliday junction resolvase RuvX -
  KN217_RS01475 (KN217_01465) - 315624..316193 (-) 570 WP_213662454.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42402.62 Da        Isoelectric Point: 6.5059

>NTDB_id=574799 KN217_RS01445 WP_008568506.1 310626..311771(+) (pilU) [Stutzerimonas stutzeri strain DN36]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKINGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEDLKLPDVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKNLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALAHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=574799 KN217_RS01445 WP_008568506.1 310626..311771(+) (pilU) [Stutzerimonas stutzeri strain DN36]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGCTCCGATCTGTTCATCACGGCCGGTGTACCGCCGTC
GATGAAGATCAACGGCAAGATCATGCCGGTGAGCAAAACGCCGATGTCGCCGGAGATGACCCGCGAAACGGTGCACGGCG
TGATGAACGAACAGCAGCGTCGCGAGTTCACCGAAAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGGCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAAACCAACATCCCGACCATCGA
AGACCTCAAGCTGCCCGATGTGCTGAAGAAGCTGGCGATGACCAAGCGCGGTCTGGTGCTGTTCGTCGGCGCCACGGGTA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCGAGCGGCCACATCATTTCCATCGAGGAC
CCGATCGAGTTCATCCACCAGCATCAGAGCTGCATCGTCACTCAGCGCGAGGTCGGCATCGACACCGAATCGTTCGATGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAGACGATGGACTACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAGCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCGGATGGCAAGGGTCGCCGTGCGGTCATCGAGGTGCTGATCAATACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGTGAGGTGCACGAGCTGAAGAATCTGATGAAGCGCTCCACCGAACTGGGTATGCAGACCTTCGACCAGGCA
CTGTACAACCTCTACGTCCAGGGCGAGATCACCTACGAAGACGCACTGGCTCATGCCGATTCGGCCAACGACCTGCGTCT
GCTGATCAAGCTGGGTTCGGAAACCGACGGCGAGCATCTGACCAGTGTGTCGCAGGGCCTGAGCCTGGAAGTCAGCGACG
ACGATCCGGGGCGTAGCTTCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

95.801

100

0.958
  pilU Acinetobacter baylyi ADP1

66.387

93.701

0.622
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391
  pilT Vibrio cholerae strain A1552

41.124

88.714

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.124

88.714

0.365
  pilT Legionella pneumophila strain Lp02

41.194

87.927

0.362
  pilT Legionella pneumophila strain ERS1305867

41.194

87.927

0.362