Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KN217_RS01440 Genome accession   NZ_CP076348
Coordinates   309536..310570 (+) Length   344 a.a.
NCBI ID   WP_008568507.1    Uniprot ID   A0A8E2QBM5
Organism   Stutzerimonas stutzeri strain DN36     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 304536..315570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KN217_RS01420 (KN217_01410) - 305261..307228 (-) 1968 WP_301619616.1 dynamin-like GTPase family protein -
  KN217_RS01425 (KN217_01415) - 307345..307935 (-) 591 WP_008568510.1 YggT family protein -
  KN217_RS01430 (KN217_01420) proC 307945..308769 (-) 825 WP_301619617.1 pyrroline-5-carboxylate reductase -
  KN217_RS01435 (KN217_01425) - 308780..309469 (-) 690 WP_213662449.1 YggS family pyridoxal phosphate-dependent enzyme -
  KN217_RS01440 (KN217_01430) pilT 309536..310570 (+) 1035 WP_008568507.1 type IV pilus twitching motility protein PilT Machinery gene
  KN217_RS01445 (KN217_01435) pilU 310626..311771 (+) 1146 WP_008568506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KN217_RS01450 (KN217_01440) - 311888..312283 (+) 396 WP_301619618.1 TM2 domain-containing protein -
  KN217_RS01455 (KN217_01445) - 312367..313638 (-) 1272 WP_301619619.1 dihydroorotase -
  KN217_RS01460 (KN217_01450) - 313638..314654 (-) 1017 WP_008568501.1 aspartate carbamoyltransferase catalytic subunit -
  KN217_RS01465 (KN217_01455) pyrR 314664..315176 (-) 513 WP_008568500.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37900.65 Da        Isoelectric Point: 6.8359

>NTDB_id=574798 KN217_RS01440 WP_008568507.1 309536..310570(+) (pilT) [Stutzerimonas stutzeri strain DN36]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGALGMQTLDACL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=574798 KN217_RS01440 WP_008568507.1 309536..310570(+) (pilT) [Stutzerimonas stutzeri strain DN36]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGCGCGTCCGACCTGCACCTTTCCTCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGGCGGATCAACCTGCCGGCGATGGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCCGGCGTCGCGCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGGACCATTCCCTCCAAGGTGCTGACGATGGA
AGACCTCGGCATGGGCGAGGTCTTCCGCAAGATCACCGACGTACCGCGCGGCCTGGTGCTGGTCACTGGCCCGACCGGCT
CGGGCAAATCGACCACGCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACCACCACATCCTTACCATCGAGGAC
CCGATCGAATTCGTTCACGAATCGAAGAAGTGCTTGGTCAACCAGCGCGAAGTGCACCGCGACACGCTGGGCTTCGCCGA
AGCGCTGCGTTCGGCGCTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACGCTGCACACCACCTCGGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCTGCCGAGGAAAAGTCGATGGTGCGCTCGATGCTGTCCGAGTCACTGCAGGCGGTGGTTTCCCAGAC
CCTGCTGAAAAAGGTCGGCGGCGGACGAGTGGCGGCGCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGATAAGGTCGCGCAGATGTACTCGTCGATCCAGACCGGTGGCGCGCTGGGCATGCAGACCCTCGATGCGTGCCTC
AAGGGCTTGCTCGCCAAGGGGCTGATCAGCCGCGACAGCGCCAAGGAAAAGGCCAAGCAGCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

98.547

100

0.985
  pilT Pseudomonas aeruginosa PAK

93.023

100

0.93
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.413

98.547

0.398
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387