Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KM539_RS15575 Genome accession   NZ_CP076250
Coordinates   3588094..3589353 (+) Length   419 a.a.
NCBI ID   WP_053839878.1    Uniprot ID   A0A0K2ZIP2
Organism   Xanthomonas translucens pv. poae strain LMG 728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3583094..3594353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM539_RS15550 (KM539_15515) - 3584171..3584467 (+) 297 WP_053833736.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KM539_RS15555 (KM539_15520) - 3584464..3584763 (+) 300 WP_237653908.1 addiction module antidote protein -
  KM539_RS15560 (KM539_15525) pilB 3584791..3586527 (-) 1737 WP_053839880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KM539_RS15565 (KM539_15530) - 3586715..3587020 (+) 306 WP_237653910.1 hypothetical protein -
  KM539_RS15570 (KM539_15535) - 3587254..3587712 (-) 459 WP_053839879.1 pilin -
  KM539_RS15575 (KM539_15540) pilC 3588094..3589353 (+) 1260 WP_053839878.1 type II secretion system F family protein Machinery gene
  KM539_RS15580 (KM539_15545) - 3589360..3590223 (+) 864 WP_053839877.1 prepilin peptidase -
  KM539_RS15585 (KM539_15550) coaE 3590237..3590857 (+) 621 WP_053839876.1 dephospho-CoA kinase -
  KM539_RS15590 (KM539_15555) - 3590980..3592314 (-) 1335 WP_053833730.1 sensor histidine kinase -
  KM539_RS15595 (KM539_15560) - 3592307..3592984 (-) 678 WP_003466405.1 response regulator transcription factor -
  KM539_RS15600 (KM539_15565) - 3593083..3593379 (-) 297 WP_237653911.1 hypothetical protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45548.73 Da        Isoelectric Point: 10.1825

>NTDB_id=574032 KM539_RS15575 WP_053839878.1 3588094..3589353(+) (pilC) [Xanthomonas translucens pv. poae strain LMG 728]
MSATRSAVSKEPVARNTSQQVPFVWEGTDKRGIKMKGEQTAKNANLLRAELRRQGITPSVVKPKPKPLFGAAGSKISAKD
IAFFSRQMATMMKSGVPIVGSLEIIGSGHKNPRMKKMVGQVRTDIEGGSSLYEAISKHPVQFDELYRNLVKAGEGAGVLE
TVLETVATYKENTEALKGKIKKALFYPAAVMGVALLVSSILLVWVVPQFEDVFKGFGAELPAFTQMIVAASRFMVAYWWL
LLIVLVSSIVGFVFAYKRSPSMQHGMDRLILKVPIIGQIMHNSSIARFARTLAVTFRAGVPLVEALDIVAGATGNSVYEK
VVLRMRDDVSVGYPVNVAMKQANLFPHMVIQMTAIGEEAGALDAMLFKVAEYFEQEVNNAVDALSSLLEPLIMVFIGTIV
GGMVVGMYLPIFKLASVVG

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=574032 KM539_RS15575 WP_053839878.1 3588094..3589353(+) (pilC) [Xanthomonas translucens pv. poae strain LMG 728]
ATGTCCGCAACTCGTAGCGCAGTATCCAAAGAACCCGTCGCACGCAATACCAGCCAGCAGGTCCCGTTCGTCTGGGAAGG
GACGGACAAGCGCGGCATCAAGATGAAGGGCGAGCAGACCGCCAAGAACGCCAACCTGCTGCGCGCCGAACTGCGCCGCC
AGGGCATCACGCCGTCGGTGGTCAAGCCGAAACCCAAGCCCTTGTTCGGTGCCGCCGGCAGCAAGATCAGCGCGAAGGAC
ATTGCCTTCTTCAGCCGCCAGATGGCGACCATGATGAAGTCGGGCGTCCCCATCGTGGGATCGCTGGAGATCATCGGCAG
CGGGCACAAGAACCCGCGCATGAAGAAGATGGTGGGCCAGGTCCGCACGGACATCGAAGGCGGCTCGTCGCTCTATGAAG
CGATCAGCAAGCATCCGGTCCAGTTCGATGAGCTCTACCGCAACCTGGTCAAGGCCGGCGAAGGCGCCGGCGTCCTCGAA
ACCGTGCTGGAGACGGTAGCCACCTACAAGGAAAACACCGAGGCGCTGAAGGGCAAGATCAAGAAAGCCTTGTTCTATCC
GGCCGCGGTCATGGGTGTGGCGCTCCTGGTCAGCTCGATCTTGCTGGTGTGGGTGGTCCCGCAATTCGAGGATGTCTTCA
AAGGGTTTGGCGCGGAGTTACCCGCATTCACTCAGATGATCGTCGCCGCTTCACGCTTTATGGTGGCTTATTGGTGGCTC
CTGCTGATCGTGCTGGTTAGCAGCATCGTCGGCTTCGTCTTCGCCTACAAGCGCTCGCCGTCGATGCAGCACGGCATGGA
CCGACTGATCCTGAAGGTGCCGATCATCGGCCAGATCATGCACAACAGCTCGATTGCTCGCTTCGCACGGACCCTCGCGG
TGACTTTCCGTGCGGGCGTCCCGTTGGTGGAGGCATTGGACATCGTCGCCGGCGCCACCGGCAACAGCGTCTACGAGAAA
GTTGTGCTGCGCATGCGCGACGACGTCTCGGTGGGCTACCCGGTCAATGTGGCGATGAAGCAGGCCAACCTGTTTCCGCA
CATGGTCATCCAGATGACCGCCATCGGCGAAGAAGCCGGTGCGCTGGATGCGATGCTGTTCAAGGTGGCCGAGTACTTCG
AGCAGGAAGTGAACAATGCGGTCGATGCGTTGAGCAGCCTGCTAGAACCGCTGATCATGGTGTTCATTGGTACCATCGTC
GGCGGCATGGTCGTCGGCATGTATCTGCCCATCTTCAAGCTGGCCTCCGTCGTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZIP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.03

94.511

0.501
  pilC Legionella pneumophila strain ERS1305867

51.733

96.42

0.499
  pilC Acinetobacter baylyi ADP1

50.735

97.375

0.494
  pilC Acinetobacter baumannii D1279779

50.245

97.375

0.489
  pilG Neisseria gonorrhoeae MS11

42.965

94.988

0.408
  pilC Vibrio cholerae strain A1552

42.222

96.659

0.408
  pilG Neisseria meningitidis 44/76-A

42.462

94.988

0.403
  pilC Vibrio campbellii strain DS40M4

40.302

94.749

0.382
  pilC Thermus thermophilus HB27

38.5

95.465

0.368