Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KM539_RS15560 Genome accession   NZ_CP076250
Coordinates   3584791..3586527 (-) Length   578 a.a.
NCBI ID   WP_053839880.1    Uniprot ID   A0A0K2ZGJ9
Organism   Xanthomonas translucens pv. poae strain LMG 728     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3579791..3591527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM539_RS15540 (KM539_15505) - 3580924..3582543 (+) 1620 WP_053839882.1 sensor histidine kinase -
  KM539_RS15545 (KM539_15510) pilR 3582618..3584006 (+) 1389 WP_053839881.1 sigma-54-dependent transcriptional regulator Regulator
  KM539_RS15550 (KM539_15515) - 3584171..3584467 (+) 297 WP_053833736.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KM539_RS15555 (KM539_15520) - 3584464..3584763 (+) 300 WP_237653908.1 addiction module antidote protein -
  KM539_RS15560 (KM539_15525) pilB 3584791..3586527 (-) 1737 WP_053839880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KM539_RS15565 (KM539_15530) - 3586715..3587020 (+) 306 WP_237653910.1 hypothetical protein -
  KM539_RS15570 (KM539_15535) - 3587254..3587712 (-) 459 WP_053839879.1 pilin -
  KM539_RS15575 (KM539_15540) pilC 3588094..3589353 (+) 1260 WP_053839878.1 type II secretion system F family protein Machinery gene
  KM539_RS15580 (KM539_15545) - 3589360..3590223 (+) 864 WP_053839877.1 prepilin peptidase -
  KM539_RS15585 (KM539_15550) coaE 3590237..3590857 (+) 621 WP_053839876.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62934.24 Da        Isoelectric Point: 6.2661

>NTDB_id=574031 KM539_RS15560 WP_053839880.1 3584791..3586527(-) (pilB) [Xanthomonas translucens pv. poae strain LMG 728]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELVHKHQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGVETGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=574031 KM539_RS15560 WP_053839880.1 3584791..3586527(-) (pilB) [Xanthomonas translucens pv. poae strain LMG 728]
ATGAACGCTGTGACATCCGCCAATCTTGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGGTGCACAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGCCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGTCGAGACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAGATCCGTGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAATTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZGJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.482

98.443

0.536
  pilB Acinetobacter baylyi ADP1

54.577

98.27

0.536
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilB Vibrio cholerae strain A1552

49.047

99.827

0.49
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.82

97.924

0.458
  pilB Vibrio campbellii strain DS40M4

46.221

98.443

0.455
  pilF Thermus thermophilus HB27

40.385

89.965

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

90.311

0.362