Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J5O00_RS13925 Genome accession   NZ_CP074415
Coordinates   2934949..2935368 (+) Length   139 a.a.
NCBI ID   WP_069514826.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 64     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2929949..2940368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5O00_RS13910 pdhR 2931981..2932748 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  J5O00_RS13915 ampD 2933154..2933705 (-) 552 WP_005479703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J5O00_RS13920 nadC 2933798..2934685 (+) 888 WP_025523107.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J5O00_RS13925 pilA 2934949..2935368 (+) 420 WP_069514826.1 pilin Machinery gene
  J5O00_RS13930 pilB 2935378..2937063 (+) 1686 WP_219079189.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J5O00_RS13935 pilC 2937087..2938310 (+) 1224 WP_029804706.1 type II secretion system F family protein Machinery gene
  J5O00_RS13940 pilD 2938375..2939244 (+) 870 WP_025501249.1 A24 family peptidase Machinery gene
  J5O00_RS13945 coaE 2939245..2939859 (+) 615 WP_005480887.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14238.22 Da        Isoelectric Point: 4.3396

>NTDB_id=564780 J5O00_RS13925 WP_069514826.1 2934949..2935368(+) (pilA) [Vibrio parahaemolyticus strain 64]
MKHSKQKKQQGFTLIELMIVVAIIGVLAAAAIPAYQNYVTRSEVTSGLATVKALITPAELHYQENGIAAAATLAQLGVDV
AANDLGAIDSALADGSPTLTFTFDADSSIDGGILTFTRDAATGWSCLPTAPAIAVDGCS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=564780 J5O00_RS13925 WP_069514826.1 2934949..2935368(+) (pilA) [Vibrio parahaemolyticus strain 64]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTAGTGGCGATTATCGGTGTATT
GGCTGCAGCTGCTATCCCTGCTTATCAAAATTATGTGACTCGCTCTGAAGTTACATCAGGGTTAGCAACCGTTAAAGCTC
TGATCACACCGGCTGAGCTGCATTACCAAGAAAATGGTATCGCGGCGGCTGCAACATTAGCCCAATTGGGAGTTGATGTT
GCAGCTAATGACTTAGGAGCTATTGATTCTGCCCTTGCAGATGGTAGTCCAACTCTGACATTTACCTTCGATGCAGACAG
TTCTATCGACGGTGGGATACTAACGTTCACAAGAGACGCTGCAACTGGTTGGTCATGTTTACCTACTGCACCAGCAATTG
CAGTCGATGGTTGCAGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

99.281

100

0.993
  pilA Pseudomonas aeruginosa PAK

47.761

96.403

0.46
  pilA Vibrio cholerae strain A1552

41.892

100

0.446
  pilA Vibrio cholerae C6706

41.892

100

0.446
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.892

100

0.446
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.552

96.403

0.381
  pilA Acinetobacter baumannii strain A118

40.157

91.367

0.367