Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KHW15_RS03775 Genome accession   NZ_CP074410
Coordinates   856572..857789 (-) Length   405 a.a.
NCBI ID   WP_024663008.1    Uniprot ID   A0A6B2AVT8
Organism   Pseudomonas syringae strain U643     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 851572..862789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW15_RS03745 (KHW15_03745) - 851944..852411 (+) 468 WP_032661062.1 hypothetical protein -
  KHW15_RS03750 (KHW15_03750) - 852702..854174 (+) 1473 WP_032661065.1 tetratricopeptide repeat protein -
  KHW15_RS03755 (KHW15_03755) - 854281..854739 (+) 459 WP_010415567.1 hypothetical protein -
  KHW15_RS03760 (KHW15_03760) yacG 854872..855081 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  KHW15_RS03765 (KHW15_03765) coaE 855078..855701 (-) 624 WP_032661069.1 dephospho-CoA kinase -
  KHW15_RS03770 (KHW15_03770) pilD 855698..856570 (-) 873 WP_032661071.1 A24 family peptidase Machinery gene
  KHW15_RS03775 (KHW15_03775) pilC 856572..857789 (-) 1218 WP_024663008.1 type II secretion system F family protein Machinery gene
  KHW15_RS03780 (KHW15_03780) pilB 857792..859486 (-) 1695 WP_024663009.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHW15_RS03785 (KHW15_03785) - 859714..860136 (+) 423 WP_024663010.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KHW15_RS03790 (KHW15_03790) - 860231..860776 (-) 546 WP_004415097.1 DUF1523 family protein -
  KHW15_RS03795 (KHW15_03795) - 860896..861894 (+) 999 WP_213569201.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43955.86 Da        Isoelectric Point: 10.1953

>NTDB_id=564681 KHW15_RS03775 WP_024663008.1 856572..857789(-) (pilC) [Pseudomonas syringae strain U643]
MASKAVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIGEGAENPNMRALVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAIIVSGILLIKVVPQFQSVFASFGADLPAFTLMVIGLSDVVQRWWLAIVGVFFASFFLLK
RAYKQSQKFRDNLDRLLLKLPIIGPLIFKSSVARYARTLATTFSAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEDEVDNMVDSLTSLMEPMIMAILGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=564681 KHW15_RS03775 WP_024663008.1 856572..857789(-) (pilC) [Pseudomonas syringae strain U643]
ATGGCCAGCAAGGCAGTCAAAGTCATCGTTTACACGTGGGAAGGTGTAGACAAGAAAGGTACTAAAACCAGTGGCGAGTT
GAGCGGCCATAACCTGGCACTCGTAAAGGCCCAACTACGCAAGCAAGGCATCAATCCGACCAAGGTGCGCAAGAAGTCTG
TATCGATATTCGGCAAAGGCAAGAAAATCAAGCCGCTGGACATTGCGTTCTTCTCTCGGCAGATGGCCACGATGATGAAA
GCCGGCGTGCCATTGCTGCAGTCTTTTGACATCATCGGCGAGGGCGCTGAAAACCCTAACATGCGTGCACTGGTGGGCTC
TCTCAAACAGGAGGTGTCGGCCGGCAACAGTTTTGCAACAGCTTTGCGACAGAAACCGGAATATTTCGATGACTTGTTTT
GCAATCTAGTGGATGCTGGCGAGCAAGCTGGCGCCCTTGAGAGCCTACTAGATAGGGTGGCAAGCTATAAGGAAAAAACC
GAGAAACTTAAAGCAAAAATCAAAAAGGCCATGACATATCCGGCTGCTGTATTGATAGTAGCAATTATTGTATCAGGCAT
TTTGCTTATCAAAGTGGTGCCGCAGTTTCAGTCAGTTTTTGCTAGTTTCGGGGCGGACTTACCCGCATTTACCTTGATGG
TTATCGGGCTTTCGGACGTAGTGCAGAGATGGTGGCTAGCAATAGTCGGAGTATTTTTCGCAAGCTTTTTTTTGCTTAAA
AGAGCTTATAAGCAATCTCAAAAATTTCGCGACAATCTGGATCGCCTTTTACTGAAATTACCCATTATAGGGCCATTGAT
TTTTAAGTCATCGGTTGCCCGCTACGCGCGCACCCTTGCAACTACCTTCTCCGCTGGTGTCCCACTGGTAGAGGCGTTAG
ATTCGGTCGCAGGGGCTACCGGCAATGTGGTATTCAAAAATGCAGTCAATAAGGTCAAACAGGACGTCTCCACGGGAATG
CAACTGAATTTCTCAATGCGCTCAACAGGGGTATTCCCCAGTCTTGCAATTCAAATGACCGCAATCGGCGAAGAATCAGG
GGCTCTGGATGCAATGCTGGACAAGGTTGCAACATATTACGAGGATGAAGTCGACAATATGGTCGATAGCCTGACCAGTC
TGATGGAGCCGATGATCATGGCGATACTGGGTGTCATCGTTGGCGGCCTTGTCATTGCGATGTACCTCCCCATTTTCAAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B2AVT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Legionella pneumophila strain ERS1305867

51.88

98.519

0.511
  pilG Neisseria meningitidis 44/76-A

43.325

98.025

0.425
  pilG Neisseria gonorrhoeae MS11

43.325

98.025

0.425
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

42.569

98.025

0.417
  pilC Thermus thermophilus HB27

39.9

99.012

0.395