Detailed information NTG0564680

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: KHW15_RS03770
• Genome accession: NZ_CP074410
• Coordinates: 855698..856570 (-)
• Length: 290 a.a.
• NCBI ID: WP_032661071.1
• Uniprot ID: A0AB35JQR3
• Organism: Pseudomonas syringae strain U643
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 850698..861570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KHW15_RS03740 (KHW15_03740)	-	851189..851818 (-)	630	WP_032661060.1	DUF1780 domain-containing protein	-
KHW15_RS03745 (KHW15_03745)	-	851944..852411 (+)	468	WP_032661062.1	hypothetical protein	-
KHW15_RS03750 (KHW15_03750)	-	852702..854174 (+)	1473	WP_032661065.1	tetratricopeptide repeat protein	-
KHW15_RS03755 (KHW15_03755)	-	854281..854739 (+)	459	WP_010415567.1	hypothetical protein	-
KHW15_RS03760 (KHW15_03760)	yacG	854872..855081 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
KHW15_RS03765 (KHW15_03765)	coaE	855078..855701 (-)	624	WP_032661069.1	dephospho-CoA kinase	-
KHW15_RS03770 (KHW15_03770)	pilD	855698..856570 (-)	873	WP_032661071.1	A24 family peptidase	Machinery gene
KHW15_RS03775 (KHW15_03775)	pilC	856572..857789 (-)	1218	WP_024663008.1	type II secretion system F family protein	Machinery gene
KHW15_RS03780 (KHW15_03780)	pilB	857792..859486 (-)	1695	WP_024663009.1	type IV-A pilus assembly ATPase PilB	Machinery gene
KHW15_RS03785 (KHW15_03785)	-	859714..860136 (+)	423	WP_024663010.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
KHW15_RS03790 (KHW15_03790)	-	860231..860776 (-)	546	WP_004415097.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32057.25 Da
• Isoelectric Point: 8.3694

>NTDB_id=564680 KHW15_RS03770 WP_032661071.1 855698..856570(-) (pilD) [Pseudomonas syringae strain U643]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAMLSAYVAWHFGYGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=564680 KHW15_RS03770 WP_032661071.1 855698..856570(-) (pilD) [Pseudomonas syringae strain U643]
ATGCCCCTCCTCGACCTACTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATCCTTGGCCTGATCATCGGAAG
CTTCCTCAATGTGGTCGTCTACCGCCTCCCGATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAACCCGTCTTCAATCTGAATCGTCCCCGTTCCAGCTGCCCGCATTGTGCACACAAAATCCGC
CCGTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGCGGTAAATGTTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAATGTTGTCGGCCTACGTAGCCTGGCATTTCGGCTATGGCTGGCAGGCGGCGG
CGATGCTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTCACCTCGCTGAATGATGCGCTATGGGGGGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGCGGTTGGCAGATTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.004	97.586	0.517
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472