Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KEF49_RS12250 Genome accession   NZ_CP073635
Coordinates   2439381..2439995 (-) Length   204 a.a.
NCBI ID   WP_212098514.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2434381..2444995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS12220 (KEF49_12220) rpsT 2434681..2434947 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  KEF49_RS12225 (KEF49_12225) holA 2434965..2436006 (-) 1042 Protein_2385 DNA polymerase III subunit delta -
  KEF49_RS12230 (KEF49_12230) - 2436046..2436200 (-) 155 Protein_2386 hypothetical protein -
  KEF49_RS12235 (KEF49_12235) - 2436241..2436375 (+) 135 WP_003152870.1 YqzM family protein -
  KEF49_RS12240 (KEF49_12240) comEC 2436394..2438745 (-) 2352 WP_212098512.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KEF49_RS12245 (KEF49_12245) - 2438746..2439315 (-) 570 WP_065981916.1 ComE operon protein 2 -
  KEF49_RS12250 (KEF49_12250) comEA 2439381..2439995 (-) 615 WP_212098514.1 helix-hairpin-helix domain-containing protein Machinery gene
  KEF49_RS12255 (KEF49_12255) comER 2440055..2440876 (+) 822 WP_065981918.1 late competence protein ComER -
  KEF49_RS12260 (KEF49_12260) - 2440945..2441682 (-) 738 WP_014470703.1 class I SAM-dependent methyltransferase -
  KEF49_RS12265 (KEF49_12265) rsfS 2441679..2442035 (-) 357 WP_115997664.1 ribosome silencing factor -
  KEF49_RS12270 (KEF49_12270) yqeK 2442052..2442612 (-) 561 WP_032856887.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KEF49_RS12275 (KEF49_12275) - 2442602..2443171 (-) 570 WP_065981920.1 nicotinate-nucleotide adenylyltransferase -
  KEF49_RS12280 (KEF49_12280) yhbY 2443182..2443472 (-) 291 WP_014470707.1 ribosome assembly RNA-binding protein YhbY -
  KEF49_RS12285 (KEF49_12285) aroE 2443466..2444308 (-) 843 WP_115997665.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22138.41 Da        Isoelectric Point: 6.9665

>NTDB_id=559603 KEF49_RS12250 WP_212098514.1 2439381..2439995(-) (comEA) [Bacillus amyloliquefaciens strain Ba13]
MTKVMMTERQRQYLKILGVCLAAGAVLICILLFRPEKEEPVKQTSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEKENPPKTAAGGKENASININTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=559603 KEF49_RS12250 WP_212098514.1 2439381..2439995(-) (comEA) [Bacillus amyloliquefaciens strain Ba13]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
GATCTGCATTTTGCTGTTTCGTCCCGAAAAAGAAGAGCCTGTCAAACAGACGTCCGTTCTCGGCCAAACAGCCTCAGCAC
CCGTAACGGACAAAAAAGACACCGCAGACGATAAAATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGAACGGTCGTTTATATCCCGAGTGAAGGCGAAAAGGAGAATCCCCCGAAAACCG
CGGCAGGTGGAAAGGAGAATGCTTCTATCAATATCAATACGGCATCTCTGGATGAACTTCAGGCGATTTCCGGAGTGGGA
CAGAAAAAAGCGGAAGCGATTATTGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.949

95.588

0.554
  comEA/celA/cilE Streptococcus mitis SK321

35.945

100

0.382
  comEA Staphylococcus aureus MW2

34.842

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae R6

35.484

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae D39

35.484

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.484

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.484

100

0.377
  comEA/celA/cilE Streptococcus mitis NCTC 12261

35.023

100

0.373
  comEA Staphylococcus aureus N315

33.937

100

0.368
  comEA Latilactobacillus sakei subsp. sakei 23K

32.051

100

0.368