Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KCG51_RS09445 Genome accession   NZ_CP073119
Coordinates   1998262..1998759 (+) Length   165 a.a.
NCBI ID   WP_254342474.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1993262..2003759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG51_RS09430 (KCG51_09460) - 1993718..1994158 (+) 441 WP_254342473.1 TIGR01244 family sulfur transferase -
  KCG51_RS09435 (KCG51_09465) - 1994595..1994891 (+) 297 WP_003684318.1 helix-turn-helix transcriptional regulator -
  KCG51_RS09440 (KCG51_09470) glnD 1994911..1997468 (+) 2558 Protein_1825 [protein-PII] uridylyltransferase -
  KCG51_RS09445 (KCG51_09475) pilE 1998262..1998759 (+) 498 WP_254342474.1 pilin Machinery gene
  KCG51_RS09450 (KCG51_09480) - 1998860..1999318 (+) 459 WP_254342475.1 pilin -
  KCG51_RS09455 (KCG51_09485) - 1999530..2001055 (-) 1526 Protein_1828 DHA2 family efflux MFS transporter permease subunit -
  KCG51_RS09460 (KCG51_09490) - 2001157..2002347 (-) 1191 WP_254342476.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 17188.71 Da        Isoelectric Point: 8.9022

>NTDB_id=558176 KCG51_RS09445 WP_254342474.1 1998262..1998759(+) (pilE) [Neisseria subflava strain HP0069]
MKAIQKGFTLIELMIVIAIIGILAVIALPAYQDYTARAQVSEAFALAEGQKAAVVEYYADKGAYPSNNAAAGVATADKIT
GKYVAKVDIGENGVITATMKKDNVNAALSEKTLTLTPKASVDATSAANNPGSFTWECGGTIDTKYRPAACRPDTATATAT
PKPKK

Nucleotide


Download         Length: 498 bp        

>NTDB_id=558176 KCG51_RS09445 WP_254342474.1 1998262..1998759(+) (pilE) [Neisseria subflava strain HP0069]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAATTGATGATCGTCATCGCGATTATCGGTATCTTGGCAGTTATCGC
CCTGCCTGCATACCAAGACTACACTGCACGCGCCCAAGTTTCTGAAGCATTTGCCTTAGCAGAAGGCCAAAAAGCTGCTG
TGGTTGAATACTATGCTGACAAAGGTGCTTACCCTAGCAACAATGCGGCTGCAGGCGTTGCGACTGCTGACAAAATTACT
GGCAAGTATGTTGCAAAAGTAGATATCGGTGAAAATGGTGTAATTACAGCCACCATGAAAAAAGATAATGTAAATGCTGC
ACTCTCTGAAAAAACATTAACTTTGACACCTAAGGCATCAGTTGATGCAACTTCTGCTGCTAATAATCCAGGTTCATTTA
CTTGGGAATGTGGTGGCACTATTGATACAAAATACCGCCCTGCCGCTTGCCGTCCTGATACTGCTACTGCTACTGCTACA
CCAAAACCAAAAAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

50.857

100

0.539
  pilE Neisseria gonorrhoeae MS11

50.888

100

0.521
  pilA2 Legionella pneumophila str. Paris

51.007

90.303

0.461
  pilA2 Legionella pneumophila strain ERS1305867

50.336

90.303

0.455
  pilA/pilA1 Eikenella corrodens VA1

43.976

100

0.442
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.979

100

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

39.521

100

0.4
  comP Acinetobacter baylyi ADP1

41.026

94.545

0.388
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.176

92.727

0.382