Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   KCG52_RS04465 Genome accession   NZ_CP073114
Coordinates   953534..954775 (+) Length   413 a.a.
NCBI ID   WP_070615539.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 948534..959775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG52_RS04450 (KCG52_04430) - 949547..950305 (+) 759 WP_254345267.1 nucleotidyltransferase domain-containing protein -
  KCG52_RS04455 (KCG52_04435) - 950320..951381 (+) 1062 WP_254345269.1 nucleotidyltransferase domain-containing protein -
  KCG52_RS04460 (KCG52_04440) pilF 951775..953454 (+) 1680 WP_254345271.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCG52_RS04465 (KCG52_04445) pilG 953534..954775 (+) 1242 WP_070615539.1 type II secretion system F family protein Machinery gene
  KCG52_RS04470 (KCG52_04450) pilD 954777..955664 (+) 888 WP_254322300.1 A24 family peptidase Machinery gene
  KCG52_RS04475 (KCG52_04455) coaE 955664..956284 (+) 621 WP_254345272.1 dephospho-CoA kinase -
  KCG52_RS04480 (KCG52_04460) yacG 956281..956463 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  KCG52_RS04485 (KCG52_04465) glyQ 956545..957444 (+) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  KCG52_RS04490 (KCG52_04470) lspA 957593..958099 (+) 507 WP_188212068.1 signal peptidase II -
  KCG52_RS04495 (KCG52_04475) ispH 958113..959081 (+) 969 WP_254345276.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45939.04 Da        Isoelectric Point: 9.8675

>NTDB_id=557993 KCG52_RS04465 WP_070615539.1 953534..954775(+) (pilG) [Neisseria subflava strain HP0048]
MAQAEQKRSLFGSRSKGKRFTFEGKNIETERLVRGEVVAKDEEEARKKLQRRGIRPLRISKVKTARKRRITQEDITVFTR
QLATMMKAGLPLMQAFEIVARGHSNPSMTEMLMQVRSDVEQGSALGKSFSKYPKYFDRFYCNLVSAGESGGVLESLLDKL
AVYKEKTQAIKKKVKTALTYPIAIIVVAIALIFIMMMFVLPAFKEVYANMGAELPDLTQLVMNLSDLFVDYGWIMIILLI
ASAFGLYKLHEKSPTFQKRIDALILRLPIFGTIVRKATIARWARTTSTLFAAGVPLVEVLDSVAGASGNILYEEATQDIR
AKVTQGLSLTSSMQSTDMFPNMVIQMAAIGEESGSLDDMLNKAAEFYEDEVDNSVSRLSALMEPIIMVVLGSLIGILLIA
MYLPLFNLGNVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=557993 KCG52_RS04465 WP_070615539.1 953534..954775(+) (pilG) [Neisseria subflava strain HP0048]
ATGGCTCAAGCAGAGCAAAAAAGAAGTCTGTTCGGTTCAAGAAGCAAAGGTAAGCGTTTTACATTTGAAGGAAAAAACAT
CGAGACCGAACGTCTTGTCCGCGGCGAAGTAGTTGCCAAAGACGAAGAAGAGGCACGCAAAAAACTCCAGCGCAGAGGCA
TTCGTCCTTTGCGCATCAGCAAAGTCAAAACCGCACGCAAACGCCGTATTACTCAAGAAGACATCACGGTGTTTACGCGC
CAACTGGCAACGATGATGAAGGCAGGCTTGCCGCTGATGCAGGCTTTTGAAATCGTTGCTCGCGGCCACTCCAATCCGAG
CATGACCGAAATGCTGATGCAGGTTCGCTCCGATGTCGAACAAGGTAGCGCATTGGGCAAATCGTTCTCCAAATATCCAA
AATATTTCGACCGTTTCTATTGCAACCTGGTCAGCGCAGGCGAATCCGGCGGCGTACTGGAAAGCTTATTGGACAAACTG
GCTGTTTATAAAGAAAAAACCCAAGCCATTAAGAAAAAGGTTAAAACCGCGCTGACCTACCCGATTGCCATTATCGTGGT
AGCCATCGCGCTTATCTTCATCATGATGATGTTTGTACTGCCTGCCTTTAAAGAAGTTTATGCCAATATGGGTGCAGAGC
TGCCAGACCTTACCCAACTCGTCATGAACTTGTCTGATTTATTTGTCGATTACGGCTGGATTATGATCATCCTTTTGATT
GCTTCCGCCTTCGGCCTGTACAAACTCCATGAGAAATCGCCCACCTTCCAAAAGCGAATCGATGCTTTGATTTTGAGGCT
GCCTATCTTCGGAACCATCGTCCGTAAAGCGACCATCGCCCGTTGGGCGCGTACAACTTCCACCCTCTTCGCGGCCGGCG
TTCCTTTGGTGGAAGTATTAGATTCAGTTGCAGGCGCATCCGGCAATATTCTCTATGAAGAAGCCACCCAAGACATCCGC
GCCAAGGTAACCCAAGGTCTGTCACTAACATCCAGTATGCAAAGTACTGATATGTTCCCTAACATGGTGATTCAAATGGC
GGCCATCGGCGAAGAGTCTGGCTCCTTGGATGATATGCTGAACAAAGCGGCTGAATTCTACGAAGATGAAGTAGACAACT
CCGTCTCCCGATTGTCTGCCTTGATGGAACCTATCATCATGGTGGTATTGGGTTCGCTCATCGGTATTTTGCTGATTGCC
ATGTATCTGCCGTTGTTCAACTTGGGTAATGTCGTAGGTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

72.525

97.821

0.709
  pilG Neisseria meningitidis 44/76-A

71.535

97.821

0.7
  pilC Acinetobacter baumannii D1279779

46.985

96.368

0.453
  pilC Acinetobacter baylyi ADP1

46.482

96.368

0.448
  pilC Pseudomonas stutzeri DSM 10701

46

96.852

0.446
  pilC Legionella pneumophila strain ERS1305867

46.096

96.126

0.443
  pilC Vibrio cholerae strain A1552

39

96.852

0.378
  pilC Thermus thermophilus HB27

38.619

94.673

0.366