Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KCG52_RS04460 Genome accession   NZ_CP073114
Coordinates   951775..953454 (+) Length   559 a.a.
NCBI ID   WP_254345271.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 946775..958454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG52_RS04450 (KCG52_04430) - 949547..950305 (+) 759 WP_254345267.1 nucleotidyltransferase domain-containing protein -
  KCG52_RS04455 (KCG52_04435) - 950320..951381 (+) 1062 WP_254345269.1 nucleotidyltransferase domain-containing protein -
  KCG52_RS04460 (KCG52_04440) pilF 951775..953454 (+) 1680 WP_254345271.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCG52_RS04465 (KCG52_04445) pilG 953534..954775 (+) 1242 WP_070615539.1 type II secretion system F family protein Machinery gene
  KCG52_RS04470 (KCG52_04450) pilD 954777..955664 (+) 888 WP_254322300.1 A24 family peptidase Machinery gene
  KCG52_RS04475 (KCG52_04455) coaE 955664..956284 (+) 621 WP_254345272.1 dephospho-CoA kinase -
  KCG52_RS04480 (KCG52_04460) yacG 956281..956463 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  KCG52_RS04485 (KCG52_04465) glyQ 956545..957444 (+) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  KCG52_RS04490 (KCG52_04470) lspA 957593..958099 (+) 507 WP_188212068.1 signal peptidase II -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62310.54 Da        Isoelectric Point: 6.0388

>NTDB_id=557992 KCG52_RS04460 WP_254345271.1 951775..953454(+) (pilF) [Neisseria subflava strain HP0048]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPDLFSDGIITPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCVPIFKRGRKVYFAVSDPTQIQNFQKIAFSSGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINEEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFATALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQNWKL
YRPVGCDSCRGKGFKGRIGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=557992 KCG52_RS04460 WP_254345271.1 951775..953454(+) (pilF) [Neisseria subflava strain HP0048]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAAACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
GCAATCGGGCAAAGAAATCCTGCCCGACTTGTTTTCAGACGGCATCATCACCCCTAAAGCCTTGGGCGAATTAGTTGCGC
GCGTATTCAGCTATCCTCTTTTGGATTTACACTATTACCCGCGCAATAATGTAGTCAGCGACATCCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCGTTCCAATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACTCAAATCCA
AAACTTCCAAAAAATCGCTTTTTCTTCAGGTTTAAGTGTTGACTTAGTGGTGGTTCCCGATGACCAACTCAGCTCCTTGC
TAGAATGGTTGGGGCAGCGTTCGACCACCATTCTGAAAGAAATCAACGAAGAACATGAAGCAACGCAGCAATCTCAGTCG
CTTTATATCGATAATGAAGAGGCTGAAGACGGCCCTATCCCCCGCTTCATCCACAAAACCTTATCCGACGCCCTAAACGC
AGGCGCATCAGACATCCACTTTGAATTCTACGAGCAAATGGCGCGCGTCCGTTTCCGCGTGGACGGACAGTTGCGCGAAG
TCGTACAGCCGCCTGTCGCCGTACGCGGTCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCTGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCACAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
GACGCTGTTTGGCGAAAAAGTGGTAATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATCAACCGCCCTTACGGTATGGTGTTGGTTACCGGCCCGACAGGCTCG
GGTAAAACCGTTTCGCTCTATACCTGCCTAAGTATTTTGAACACGGAAGATGTGAACATTTCAACAGCAGAGGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGCCTGACGTTTGCCACCGCACTCCGATCCT
TCTTGCGTCAAGATCCTGATATTATTATGGTCGGTGAGATCCGTGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCTACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
GGCGCCTTTCAATATTGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTGGAGCGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAAATTGGAAGCTC
TACCGCCCGGTTGGTTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCATCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACCTGCGTCGCGCCGGCCTCATGAAAGTCATGCAGGGCCTGACTTCGTTGGAAGAAGTAACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

76.923

100

0.769
  pilB Acinetobacter baylyi ADP1

50.35

100

0.515
  pilB Acinetobacter baumannii D1279779

49.912

100

0.504
  pilB Legionella pneumophila strain ERS1305867

47.35

100

0.479
  pilB Vibrio cholerae strain A1552

46.454

100

0.469
  pilB Vibrio campbellii strain DS40M4

44.092

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

46.717

95.349

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.447

96.78

0.372