Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LZ759_RS10050 Genome accession   NZ_CP090510
Coordinates   2252347..2252793 (+) Length   148 a.a.
NCBI ID   WP_071869805.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain LA-Y3C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2247347..2257793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ759_RS10030 (LZ759_10030) - 2247595..2248932 (+) 1338 WP_027700147.1 HAMP domain-containing sensor histidine kinase -
  LZ759_RS10035 (LZ759_10035) coaE 2249199..2249822 (-) 624 WP_027700146.1 dephospho-CoA kinase -
  LZ759_RS10040 (LZ759_10040) pilD 2249834..2250697 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  LZ759_RS10045 (LZ759_10045) pilC 2250704..2251906 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  LZ759_RS10050 (LZ759_10050) pilA 2252347..2252793 (+) 447 WP_071869805.1 pilin Machinery gene
  LZ759_RS10055 (LZ759_10055) - 2253038..2253265 (-) 228 WP_012338102.1 hypothetical protein -
  LZ759_RS10060 (LZ759_10060) pilA/pilAI 2254098..2254544 (+) 447 WP_071869804.1 pilin Machinery gene
  LZ759_RS10065 (LZ759_10065) pilB 2255384..2257117 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15372.58 Da        Isoelectric Point: 8.4988

>NTDB_id=554562 LZ759_RS10050 WP_071869805.1 2252347..2252793(+) (pilA) [Xylella fastidiosa subsp. multiplex strain LA-Y3C]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKATSNASDIGLRTDTTRCGI
TVKVEAAGTANITCKVKGNSQVSDKTIAWDRTPDNSAGTNGVNNGGVWTCSTTVTSDALRPSGCIATK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=554562 LZ759_RS10050 WP_071869805.1 2252347..2252793(+) (pilA) [Xylella fastidiosa subsp. multiplex strain LA-Y3C]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGGCTACATCCAATGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGAATT
ACCGTTAAAGTCGAGGCCGCTGGCACCGCCAACATCACATGCAAAGTTAAGGGCAACTCTCAAGTCAGTGATAAAACCAT
CGCTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCAACCACCG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATTGCCACTAAATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40

100

0.419
  pilA Vibrio cholerae strain A1552

40

100

0.419
  pilA Vibrio cholerae C6706

40

100

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.497

99.324

0.412
  pilA Pseudomonas aeruginosa PAK

37.736

100

0.405
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.816

99.324

0.405
  pilA Acinetobacter baumannii strain A118

38

100

0.385
  pilA Vibrio parahaemolyticus RIMD 2210633

36.486

100

0.365