Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   J4N37_RS13135 Genome accession   NZ_CP071855
Coordinates   2771627..2772850 (+) Length   407 a.a.
NCBI ID   WP_045393852.1    Uniprot ID   A0AAW6ZYM1
Organism   Vibrio sp. SCSIO 43153     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2766627..2777850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N37_RS13115 (J4N37_12985) ampD 2767709..2768260 (-) 552 WP_045393860.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J4N37_RS13120 (J4N37_12990) nadC 2768353..2769240 (+) 888 WP_045393857.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J4N37_RS13125 (J4N37_12995) - 2769468..2769926 (+) 459 WP_045393855.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  J4N37_RS13130 (J4N37_13000) pilB 2769926..2771611 (+) 1686 WP_010450185.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4N37_RS13135 (J4N37_13005) pilC 2771627..2772850 (+) 1224 WP_045393852.1 type II secretion system F family protein Machinery gene
  J4N37_RS13140 (J4N37_13010) pilD 2772921..2773790 (+) 870 WP_045393848.1 A24 family peptidase Machinery gene
  J4N37_RS13145 (J4N37_13015) coaE 2773791..2774405 (+) 615 WP_038885728.1 dephospho-CoA kinase -
  J4N37_RS13150 (J4N37_13020) zapD 2774434..2775174 (+) 741 WP_045393845.1 cell division protein ZapD -
  J4N37_RS13155 (J4N37_13025) yacG 2775231..2775431 (+) 201 WP_252041145.1 DNA gyrase inhibitor YacG -
  J4N37_RS13160 (J4N37_13030) rplS 2775802..2776155 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  J4N37_RS13165 (J4N37_13035) trmD 2776197..2776940 (-) 744 WP_005425529.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  J4N37_RS13170 (J4N37_13040) rimM 2776968..2777516 (-) 549 WP_005534055.1 ribosome maturation factor RimM -
  J4N37_RS13175 (J4N37_13045) rpsP 2777544..2777792 (-) 249 WP_005438039.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45172.38 Da        Isoelectric Point: 10.4164

>NTDB_id=548417 J4N37_RS13135 WP_045393852.1 2771627..2772850(+) (pilC) [Vibrio sp. SCSIO 43153]
MKVSTPQLKNFRWKGINSSGKQTSGHILALNEVEVRERLDAQHIKVKKLKRSNASVLSKLSNRVKARDITIFTRQITTML
TTGVPIVQALKLVSENHRKAEMKSILLNISKAVEAGTPMSKAMRTSSQHFDPLYTDLIATGEQSGNLDKVFERLATYREK
SEQLRAKVIKALIYPAMVILVALIVSFVMLTKVIPEFQKMFAGFGANLPWFTQQILALSDWVQNWGGIMVLGIVGVIISC
KLISNRSPSFHLFLARTSLKIPVLGPVFSKAAIAKFSRTLATSFSSGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIANIYEFEVDNTVDNLSKILEPLIILFLGVVVGSMVAAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=548417 J4N37_RS13135 WP_045393852.1 2771627..2772850(+) (pilC) [Vibrio sp. SCSIO 43153]
ATGAAAGTATCAACACCCCAACTTAAGAACTTCCGCTGGAAAGGCATCAATAGCTCAGGAAAACAAACATCAGGCCATAT
ACTCGCTCTGAATGAGGTCGAGGTTCGCGAAAGACTCGATGCTCAACACATCAAAGTTAAAAAACTGAAAAGAAGCAATG
CTTCTGTCTTATCAAAATTAAGTAACAGAGTTAAAGCCAGAGACATCACGATTTTCACTCGCCAGATTACAACTATGCTG
ACAACCGGTGTTCCTATTGTCCAAGCGCTCAAGTTGGTGTCAGAAAATCACCGCAAAGCGGAAATGAAATCCATACTGTT
GAACATTAGTAAAGCAGTAGAGGCAGGAACCCCCATGTCAAAAGCAATGAGAACTTCAAGCCAGCACTTCGACCCGCTGT
ATACAGATCTCATTGCCACTGGAGAACAATCCGGTAATTTAGATAAAGTTTTTGAACGCCTCGCTACCTACCGGGAAAAA
AGCGAGCAGCTTCGAGCTAAAGTTATCAAGGCTCTGATTTATCCCGCCATGGTGATCTTAGTAGCGCTAATTGTATCTTT
TGTCATGCTTACCAAAGTAATACCTGAGTTCCAAAAAATGTTTGCTGGCTTTGGCGCCAATTTACCTTGGTTTACCCAAC
AGATCCTAGCCCTATCAGATTGGGTTCAAAATTGGGGAGGGATTATGGTTTTAGGAATTGTGGGCGTTATTATTTCATGC
AAGCTAATCAGCAATCGTTCTCCTTCTTTCCATCTATTTTTAGCGCGAACGAGCTTGAAAATCCCAGTCCTAGGACCAGT
TTTTTCAAAAGCGGCAATAGCTAAATTTAGTAGAACACTAGCCACTAGTTTTAGTTCCGGCATTCCGATACTCACGAGTT
TAAAAACCACCTCAAAAACCTCTGGCAACCTACACTATCAACTCGCAATTGAAGAGGTCTATCGTGATACTGCTGCAGGG
ATGCCAATGTACATTGCGATGAGAAACTGCAATGTATTTCCAGAGTTAGTATTGCAAATGGTTATGATTGGCGAAGAATC
CGGAAGGCTTGACGATATGCTCAATAAAATAGCCAATATTTATGAGTTCGAAGTCGACAACACCGTAGATAACTTAAGCA
AAATCTTAGAGCCTTTAATCATCTTATTTTTAGGGGTGGTTGTCGGCAGTATGGTTGCTGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

80.344

100

0.803
  pilC Vibrio cholerae strain A1552

71

98.28

0.698
  pilC Acinetobacter baumannii D1279779

42.25

98.28

0.415
  pilC Acinetobacter baylyi ADP1

41.058

97.543

0.4
  pilC Legionella pneumophila strain ERS1305867

38.424

99.754

0.383
  pilC Pseudomonas stutzeri DSM 10701

39.394

97.297

0.383
  pilG Neisseria meningitidis 44/76-A

38.596

98.034

0.378
  pilG Neisseria gonorrhoeae MS11

38.346

98.034

0.376